Search Within
Applications
Content Type
PCR
Applied Filters:
facet applications:PCR
facet content type:Technical Article
Showing 1-30 of 59 results
ThermaStop™ PCR Additive
PCR additive added to master mix that stops nonspecific bands after cool down.
Long and Accurate PCR
Long and accurate PCR applications address the needs for longer read lengths, greater fidelity and higher yields than that which can be achieved with Taq DNA polymerase.
A highly efficient method to concentrate DNA for forensic STR genotyping using DNAstable®
Forensic laboratories routinely use STR genotyping for identity testing of biological samples. However, forensic samples often contain low copy numbers of target DNA, making it difficult to obtain complete STR profiles.
Optimizing Crude Sample Plant PCR
Overcoming potent inhibitors and sample diversity in direct plant PCR.
ThermaGenix Product Overview
How to stop nonspecific bands and off target primers in PCR.
FastStart™ Taq DNA Polymerase, 5 U/μL Protocol & Troubleshooting
The choice of the PCR enzyme in combination with an appropriate buffer can profoundly affect PCR outcome.
PCR Assay Optimization and Validation
PCR assay guide navigates you through primer validation and other assay optimization factors to ensure high sensitivity and specificity for optimum DNA/ RNA quantification.
Complete Solutions for PCR Assay Development
Fit-for-use products offer the quality, consistency & documentation necessary for every step of your IVD development and manufacturing process.
Oligonucleotide Quality Control & Quality Assurance
The foundation of Sigma's manufacturing process is a robust QMS, which drives compliance to ISO 9001:2008 certification.
Custom LAMP Primers in Partnership with New England Biolabs
Primers manufactured using proprietary purification adjustments to perform optimally in loop-mediated isothermal amplification assays.
Working with PCR
General Considerations; Factors to Consider in RT-PCR; Prevention of Carryover Contamination; Troubleshooting
KAPA Express Extract Kit FAQs
Extract DNA from crude samples for PCR applications, including qPCR, using the KAPA Express Extract Kit. See frequently asked questions regarding sample type, optimization and storage.
KAPA2G Robust PCR Kits FAQs
Review some of the most asked questions regarding the KAPA2G Robust PCR Kits, which consists of KAPA2G Robust DNA Polymerase to address inconsistent amplification and KAPA2G Robust ReadyMix.
Mycoplasma PCR ELISA Protocol
Mycoplasmas are potential contaminants in mammalian cell culture manufacturing. All products produced in cell culture to be tested for the presence of Mycoplasma
GC-RICH PCR System Troubleshooting
GC-RICH Amplification of Polymerase Chain Reaction (PCR) System Troubleshooting.
Extract-N-Amp™ Blood PCR Kit Protocol
The Extract-N-Amp Blood PCR Kits contain all the reagents needed to rapidly extract and amplify human genomic DNA from whole blood, whole blood dried on a blood card, and cultured mammalian cells.
RT-PCR Troubleshooting
PCR troubleshooting guide for reliable data in molecular assays.
PCR Master Mix
PCR master mix simplifies PCR/RT-PCR with components like DNA polymerase, dNTPs, MgCl2, and buffer, available commercially or DIY.
PCR Technologies Guide
Basic PCR/qPCR/dPCR protocols for assay quality control and specific studies.
FastStart™ Taq DNA Polymerase Protocol & Troubleshooting
PCR success relies on enzyme, buffer choice, template purity, primer concentration, and nucleotide quality.
Cloning Troubleshooting
Molecular cloning inserts genes into plasmid vectors for protein expression in different studies.
Introduction & Historical Timelines
Explore PCR's history, from discovery to Nobel Prize. Discover real-time PCR (qPCR) and digital PCR developments.
Water for PCR Techniques
Learn how water impurities can affect PCR and related methodologies. High purity water purified using ultrafiltration is suitable for PCR and related techniques.
PCR Troubleshooting Tips
PCR troubleshooting protocols require exploring all potential error sources to ensure reliable results, addressing each problem independently.
Extract-N-Amp™ Plant PCR Kits
Extract-N-Amp kits extract and amplify genomic DNA, optimized for plant tissue without inhibition concerns.
Whole Genome Amplification for Single Cell Biology
Whole genome amplification overcomes restrictions for single-cell genomic analyses with non-specific amplification.
WTA2 Amplicon Modification for NGS
Transplex Whole Transcriptome Amplification (WTA2) precisely amplifies RNA maintaining transcript levels in test and reference samples.
Locked Nucleic Acid & Minor Groove Binder / Eclipse Dark Quencher
Explore Locked Nucleic Acid and MGB. These oligo modifications offer stability and specificity in molecular analysis, making them ideal for research and diagnostic applications.
Quantitative PCR and Digital PCR Detection Methods
Fluorescent dyes or probes are included in PCR mixes to monitor the change in NA amplicon concentration as the reaction proceeds.
Nuclease-Free Water at Your Fingertips
An ultrafiltration cartridge can be placed at the outlet of a water purification system to deliver nuclease-free ultrapure water.
Page 1 of 2