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Applications:Peptide synthesis
Biotinylation Reagents
Biotin-labelled peptides have many important applications in immunology and histochemistry, such as affinity purification and FRET-based flow cytometry, solid-phase immunoassays, and receptor localization, that exploit the high affinity of streptavidin and avidin for biotin.
Boc Resin Cleavage and Deprotection
The most popular reagent for cleavage of peptides from Boc-based resins is anhydrous HF. Of all the cleavage procedures HF appears to be the most versatile and least harmful to a wide variety of peptides synthesized on Boc-based resins.
Selecting Orthogonal Building Blocks
Novabiochem® product range has one of the largest collections of orthogonally and quasi-orthogonally protected tri-functional amino acids. These derivatives are useful tools for the synthesis of cyclic and branched peptides and peptides carrying side-chain modifications.
Purer Fmocs Means Purer Peptides
Purer Fmocs Means Purer Peptides
Heterocycle and Cyclic Peptide Formation with N-(isocyamino)triphenylphosphorane (Pinc)
Isocyanides are widely used reagents in organic synthesis, with applications ranging from materials science to drug discovery.
Peptide Coupling Reagents Selection Guide
Novabiochem® offers a large number of coupling reagents for in situ activation. In situ activating reagents are easy to use, fast reacting – even with sterically hindered amino acids, and their use is generally free of side reactions.
Overcoming Aggregation in Solid-phase Peptide Synthesis
The ease of assembly of a given peptide sequence is hard to predict, which makes peptide synthesis challenging. Review methods and reagents for avoiding aggregation in solid-phase peptide synthesis.
Solvent Systems for Thin-layer Chromatography of Novabiochem Products
A guide to create solvent systems used for the thin-layer chromatography assay of Novabiochem products.
Base Resins for Peptide Synthesis
The Novabiochem® product line has one of the most extensive ranges of polymer-supports for solid phase peptide synthesis. They range from high-loaded, lows welling for the large-scale production of relatively short peptides to high-swelling, low-loaded for the synthesis of long
Peptide Synthesis
Peptide synthesis involves the formation of a peptide bond between two amino acids to create a peptide composed of a chain of multiple amino acids.
Protocols for the Fmoc SPPS of Cysteine-containing Peptides
Overcome challenges in synthesis and disulfide bond formation with protocols for Fmoc solid-phase peptide synthesis of peptides with cysteine and methionine.
Linkers for Fmoc SPPS
Novabiochem® has one of the most extensive ranges of linkers and derivatized resins for Fmoc solid phase peptide synthesis. These resins have varied properties with special protocols for loading and cleaving.
Unnatural Amino Acids
Unnatural amino acids, the non-proteinogenic amino acids that either occur naturally or are chemically synthesized, are becoming more and more important as tools for modern drug discovery research.
Solving Aspartimide Formation in Fmoc SPPS with Fmoc-Asp(OBno)-OH
Aspartimide formation 1,2 is caused by repeated exposure of aspartic acid-containing sequences to bases like piperidine and can result ultimately in the generation of 9 different by-products.
Chemoselective Purification Tags
Our long peptide purification utilizes a combination of chemoselective purification tags and standard RP-HPLC. The method is especially effective at removing impurities that are closely eluting or hidden under the isolated product peak
Peptide Labeling
Chromogenic and fluorogenic derivatives are invaluable tools for biochemistry, having numerous applications in enzymology, protein chemistry, immunology and histochemistry.
Building Blocks for Introducing Post-translational Modified Amino Acids
We provide an overview of our available reagents, together with recommendations and details of their use for synthesis of peptides containing post-translationally modified amino acids.
Peptide Resin Loading Protocols
Review methods and resins for attaching amino acids and peptides, including Merrifield, trityl-based, and hydroxymethyl-functionalized resins. Resin-immobilized peptides can be used for various downstream applications.
Fmoc Resin Cleavage and Deprotection
Fmoc resin cleavage and deprotection follows the difficult task of detaching the peptide from the resin support and removing all the side-chain protecting groups of the amino acid residues to yield the desired peptide.
Proline Derivatives and Analogs
Proline analogues are promising candidates for tuning the biological, pharmaceutical, or physicochemical properties of naturally occuring, as well as de novo designed, linear, and, cyclic peptides.