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Protein and nucleic acid interactions
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Showing 1-14 of 14 results
Duolink® PLA Multicolor Detection Protocol
Duolink® PLA Multicolor Detection Protocol
Counterstaining after the Duolink In Situ Protocol
We recommend applying the counterstaining protocol after the completion of the Amplification step in section 7.3, step 5 of the Duolink In Situ Fluorescence User Manual.
Duolink® PLA Probemaker Guide
Guide to create custom PLA probes for immunofluorescent or brightfield detection.
Purification or Removal of DNA-Binding Proteins
This page shows how to purify or remove DNA-binding proteins with Heparin Sepharose High Performance, Heparin Sepharose 6 Fast Flow, Capto Heparin from Cytiva.
Creating PLA® Probes
Duolink® kits use in situ PLA® technology for accurate quantification of individual proteins, interactions, and modifications in cells and tissue.
Synthetic Peptide Handling & Storage Protocol
Solvent choice is crucial for synthetic peptide handling, with guidelines provided for proper dissolution.
Northern & Southern Blot Protocols
Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.
Using Duolink™ in Multiwell Plates
Duolink protocol modifications for staining cells in multiwell plates; adapt washing, imaging, and scanning for multiwell format.
Chromatin Isolation by RNA Purification (ChIRP) Protocol
Chromatin Isolation by RNA purification (ChIRP) protocol for isolating RNA-bound genome regions.
Using Duolink® in Protein Interaction Studies
The video follows the simple and straightforward procedure that allows you to detect, quantify and obtain cell localization of protein interactions and their modifications in a single experiment.
Protocol for Anti Ago-RNA Immunoprecipitation from Mammalian Cells Using the RIP Kit
Procedure and protocol for Anti Ago-RNA Immunoprecipitation from mammalian cells using the RIP kit
Duolink® In Situ Short Instructions - Fluorescence
This page details the Duolink® In Situ Short Protocol for fluorescence detection
RNA Immunoprecipitation qPCR (RIP- qPCR) Protocol
RNA immunoprecipitation (RIP) can identify specific RNA molecules of many types associated with specific nuclear or cytoplasmic binding proteins.
Duolink® PLA Brightfield Protocol
Duolink® PLA reagents enable brightfield detection and quantification of proteins and interactions in tissue samples.