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Immunoprecipitation Kit (Protein A) Protocol & Troubleshooting
Immunoprecipitation Kit (Protein A) Protocol & Troubleshooting
Imprint® Chromatin Immunoprecipitation Kit (CHP1) Protocol
The Sigma Imprint Chromatin Immunoprecipitation Kit uses a plate based system to allow rapid ChIP assays in a high throughput format
Immunoprecipitation Procedure
To determine the molecular weights of protein antigens, to study protein/protein interactions, to determine specific enzymatic activity, to monitor protein post-translational modifications and to determine the presence and quantity of proteins.
Dextran Sulfate
Dextran sulfates are supplied as the sodium salt forms, making them soluble and stable in water. Dextran sulfate contains approximately 17% sulfur which is equivalent to approximately 2.3 sulfate groups per glucosyl residue.
Purification from Unclarified Cell Lysate using HisTrap™ FF Crude
This page shows how to purify histidine-tagged recombinant proteins from unclarified cell lysate using HisTrap FF crude from Cytiva.
Antibody Purification using Protein A, Protein G, or Protein L Agarose
Antibody Purification using Protein A, Protein G, or Protein L Agarose protocol is designed as a quick purification method for antibodies from mammalian sera, ascites, and cell culture supernatants. It should be noted that if the starting material is serum
Protocols for Membrane Scaffold Proteins and Nanodisc Formation
The following material related to Nanodisc Technology is adapted from on-line content of the research group of Professor Stephen Sligar of the University of Illinois at Urbana-Champaign, with the kind permission of Professor Sligar.
Immunoprecipitation Kit (Protein G) Protocol & Troubleshooting
Immunoprecipitation Kit (Protein G) Protocol & Troubleshooting
Sera-Mag™ SpeedBeads Magnetic Protein A/G-Particles Protocol
Sera-Mag SpeedBeads Protein A/G Magnetic Particles provide a fast and convenient method for both manual and automated magnetic isolation of proteins using affinity binding. The particles can be used for isolating antibodies from serum, cell culture supernatant or ascites, and
Performing a Purification of IgG Antibodies with Protein G HP SpinTrap™/Ab SpinTrap™
This page shows how to separate IgG antibodies by affinity chromatography using Protein G HP SpinTrap™/Ab SpinTrap™ from Cytiva.
Performing a Purification of IgG Antibodies with MabSelect Sure
This page shows how to separate IgG antibodies by affinity chromatography using MabSelect Sure from Cytiva.
Dextran
Dextran is a polymer of anhydroglucose. It is composed of approximately 95% alpha-D-(1-6) linkages. The remaining a(1-3) linkages account for the branching of dextran. Conflicting data on the branch lengths implies that the average branch length is less than three
Performing a Separation with Cytiva Products Based on GST Fusion Proteins
How to perform a separation with GST fusion proteins which are affinity chromatography tags used in GST MicroSpin Purifcation Module, GSTrap FF, GSTPrep FF 16/10, Glutathione Sepharose 4 Fast Flow and Glutathione Sepharose 4B products from Cytiva.