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Antibody Purification using Protein A, Protein G, or Protein L Agarose
Antibody Purification using Protein A, Protein G, or Protein L Agarose protocol is designed as a quick purification method for antibodies from mammalian sera, ascites, and cell culture supernatants. It should be noted that if the starting material is serum...
Affinity Purification with Ultrafree®-MC Centrifugal Filters
Affinity interaction chromatography is an effective method for protein purification that can achieve up to 95% purity in one step
Purification or Removal of Biotin and Biotinylated Biomolecules with Magnetic Beads
This page shows how to Purify or remove biotin and biotinylated biomolecules with magnetic beads.
Performing a Purification of Antibody Fragments with Capto™ L Products
This page shows how to purify antibody fragments by affinity chromatography using Capto L products from Cytiva.
Gel Filtration Markers Kit for Protein Molecular Weights 12,000-200,000 Da
Gel filtration chromatography is an established method for determining the size and molecular mass of proteins.
Capto Core 700
Capto Core 700 from Cytiva is designed for intermediate purification and polishing of viruses and other large biomolecules in flowthrough mode.
General Instructions for Affinity Purification Using HiTrap® Columns
General instructions for affinity purification using HiTrap columns from Cytiva.
Performing a Separation with HiTrap® IgM Purifcation HP
How to perform a separation with the HiTrap® IgM Purifcation HP from Cytiva which is an Affinity Chromatography product optimized for purification of monoclonal IgM from hybridoma cell culture.
Removal of Albumin Using Blue Sepharose® Chromatography Media
Remove albumin from affinity chromatography samples using HiTrap™ Blue HP or Blue Sepharose® 6 Fast Flow from Cytiva.
Column Packing and Preparation Protocol for Affinity Chromatography
This page shows how to perform column packing and preparation for affinity chromatography when using Tricorn™ or XK columns available from Cytiva.
Removal of Thrombin and Factor Xa Using HiTrap® Benzamidine FF (High Sub)
To protect the fusion protein from proteolytic degradation prior to enzymatic cleavage with PreScission Protease, thrombin, or Factor Xa, it may be necessary to remove proteases from the sample. Additionally, following enzymatic cleavage, it may be necessary to remove thrombin...
HIC Purification Options and Scale up with SOURCE™ Media
This page discusses how to do a purification with SOURCE™ media from Cytiva.
Cell Disruption and Membrane Preparation
This page shows how to perform a cell disruption und prepare membranes with products from Cytiva.
Refolding of proteins using gel filtration
This page shows how to refold proteins using gel filtration and products from Cytiva.
Selectivity and the Properties of a HIC Medium
Selectivity of HIC medium from Cytiva is based on properties such as hydrophobicity, porosity and particle size.
Sepharose® High Performance: Purification with High Resolution
Here we discuss purification options when working with Sepharose® High Performance and we present some examples of media screening, capture and purification.
Purification of Histidine-Tagged Proteins Secreted into Eukaryotic Cell Culture Supernatants Using HisTrap™ Excel
This page shows how to purify histidine-tagged proteins secreted into eukaryotic cell culture supernatants using HisTrap Excel from Cytiva.
Purification of Histidine-Tagged Proteins using TALON® Superflow
This page shows how to purify histidine-tagged proteins using TALON Superflow from Cytiva.
Performing a Separation of DNA binding proteins with Cytiva Products Based on Heparin
This page shows how to use heparin in the separation of DNA binding proteins used in HiTrap Heparin HP, HiPrep 16/10 Heparin FF and Heparin Sepharose 6 Fast Flow products from Cytiva.
Performing a Purification of Glycoproteins and Polysaccharides with Agarose Wheat Germ Lectin
This page shows how to purify glycoproteins and polysaccharides by affinity chromatography using Agarose Wheat Germ Lectin.
Performing a Purification of NAD+-dependent dehydrogenases and ATP-dependent kinases with 5’ AMP Sepharose® 4B, HiTrap® Blue HP and Blue Sepharose® 6 Fast Flow
This page shows how to purify NAD+-dependent dehydrogenases and ATP-dependent kinases by affinity chromatography using 5’ AMP Sepharose® 4B, HiTrap® Blue HP and Blue Sepharose® 6 Fast Flow products from Cytiva.
Column Packing and Preparation for Size Exclusion Chromatography
Column Packing and Preparation for Size Exclusion Chromatography
Capto Adhere ImpRes
Capto adhere ImpRes is a cost-effective and flexible chromatography medium from Cytiva, designed for high-resolution polishing of MAbs.
Purification of Histidine-Tagged Recombinant Proteins Using Ni Sepharose® High Performance
Ni Sepharose High Performance consists of highly cross-linked 6% agarose beads (34 µm) to which a chelating group has been immobilized and subsequently charged with Ni2+ ions.
Minipreps using His SpinTrap™ and His SpinTrap™ Kit
This page describes a small-scale purification (minipreps) of histidine-tagged recombinant proteins using His SpinTrap and His SpinTrap Kit from Cytiva.
Purification from Unclarified Cell Lysate using HisTrap™ FF Crude
This page shows how to purify histidine-tagged recombinant proteins from unclarified cell lysate using HisTrap FF crude from Cytiva.
Magnetic Bead-Based Purification/Screening Using His Mag Sepharose® Ni
This page shows magnetic bead-based purification and screening using His Mag Sepharose Ni from Cytiva.
Removal of GST Tag by Enzymatic Cleavage
This page how to remove GST tags by enzymatic cleavage using Cytiva products.
Fractional Precipitation Protocol for Affinity Chromatography Samples
This page shows describes fractional precipitation for removing gross impurities from affinity chromatography samples with small volumes.
Purification using StrepTactin Sepharose® High Performance
This page shows how to purify Strep-tag II recombinant proteins using StrepTactin Sepharose® High Performance from Cytiva.

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