Applications
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Ion Exchange Chromatography Troubleshooting
This page covers practical problems that may lead to a non-ideal IEX separation and their solutions.
Chelators
BioUltra Chelators. A chelate is a water-soluble complex between a metal ion and a complexing agent. It usually does not dissociate easily in solution, but forms an inert complex.
Crystallization
The elucidation of a macromolecular structure to the atomic level by X-ray or neutron diffraction analysis requires the molecule to be available in the form of relatively large single crystals. Many soluble proteins, membrane proteins, nucleic acids and nucleoprotein complexes...
Automated IgG Purification Using PureProteom
PureProteome™ Protein A and G Magnetic beads provide a rapid and reproducible means to purify immunoglobulins (IgG) using the KingFisher Duo particle processor.
RPC Purification Options and Scale up with SOURCE™ Media
The different stages of working with SOURCE™ (Cytiva) for high-resolution separation are discussed, from purification to polishing and scaling up
Centrifugation-free Protein Extraction and Purification from Total Cultures of Baculovirus-infected Insect Cells
Centrifugation-free Protein Extraction and Purification from Total Cultures of Baculovirus-infected Insect Cells
Anti-FLAG® M2 Magnetic Beads
Comparison of elution techniques for small-scale protein purification of FLAG® tag proteins using anti-FLAG® M2 magnetic beads.
Protein Labeling and Purification with Amicon® Pro Centrifugal Filters
Commercially available protein labeling kits provide researchers with the flexibility to customize their own immunoassay detection panels.
Manual and Automated Purification
This page describes manual and automated purification of histidine-tagged recombinant proteins using Cytiva products
Yeast Cell Lysis Reagents
Our yeast cell lysis reagents are versatile, phosphate-free, and non-denaturing. CelLytic Y Cell Lysis reagent provides an effective method for yeast cell lysis and protein solubilization. The CelLytic Y Yeast Enzymatic Lysis Kit provides a convenient method for...
The Potential of Dendritic Polyester Scaffolds as Biocompatible Drug Delivery Agents
Professor Michael Malkoch provides an overview of the unique properties of dendritic molecules and their application in biomedical fields. He highlights bis-MPA dendritic scaffolds as a promising biodegradable and biocompatible platform for drug delivery applications
Organelle Isolation
The isolation of subcellular fractions by centrifugation is a commonly used technique and is widely applicable across multiple cell and tissue types. Because organelles differ in their size, shape, and density, centrifugation can be easily employed to separate and purify...
Capto MMC
Capto MMC is a multimodal cation exchanger with the properties of a weak cation exchanger. In addition to electrostatic interactions, the ligand structure provides for additional interaction modes such as hydrophobic interaction, hydrogen bonding, and thiophilic interaction.
Maintenance of Multimodal Chromatography Media and Storage Conditions
This page describes the maintenance of media and storage conditions for multimodal chromatography using Cytiva products.
Desalting and Buffer Exchange for Affinity Chromatography of Antibodies
Desalting at laboratory scale is a well-proven, simple, and fast method that will rapidly remove low molecular weight contaminants at the same time as transferring the sample into the desired buffer in a single step.
Purification or Removal of Serine Proteases, such as Thrombin and Trypsin, and Zymogens
This page shows how to Purify or remove serine proteases with a Benzamidine Sepharose 4 Fast Flow, Benzamidine Sepharose 4 Fast Flow from Cytiva.
Cleavage and Purification of GST-Tagged Protein Bound to Glutathione Sepharose in Batch Mode
This page shows how to cleave and purify GST-tagged proteins bound to Glutathione Sepharose in batch mode from Cytiva. Glutathione Sepharose High Performance, Glutathione Sepharose 4 Fast Flow, and Glutathione Sepharose 4B can all be used for cleavage and purification...
Cleavage and Purification of GST-Tagged Protein Bound to GSTrap
This page shows how to cleave and purify GST-tagged proteins bound to GSTrap from Cytiva.
Column Packing and Preparation for Hydrophobic Interaction and Reversed Phased Chromatography
This page discusses column packing and preparation techniques for reverse phase chromatography.
HIC as a Capture Step
This page shows Hydrophobic Interaction Chromatography (HIC) in a purification strategy.
Performing a Purity and Homogeneity Check
This page shows how to perform a purification and homogeneity check of membrane proteins with products from Cytiva.
Troubleshooting Detection Methods for GST-tagged Proteins
This page describes troubleshooting strategies in the detection of GST-tagged proteins.
Immobilized Metal Chelate Affinity Chromatography (IMAC)
This page shows how to separate proteins and peptides with affinity for metal ions by immobilized metal chelate affinity chromatography using HiTrap Chelating HP, Chelating Sepharose Fast Flow,His MicroSpin Purification Module or HisTrap Kit from Cytiva.
Performing a Separation with IgG Sepharose 6 Fast Flow
Perform a separation with IgG Sepharose 6 Fast Flow from Cytiva, an Affinity Chromatography product for purification of recombinant fusion proteins containing a protein A tail.
Performing a Separation with HiTrap® Protein G HP
Purify monoclonal or polyclonal IgG from serum, cell culture supernatant or ascitic fluid using the HiTrap Protein G HP from Cytiva, an affinity-exclusion chromatography product containing Sepharose-immobilized Protein G.
Performing a Purification of NADP+-dependent dehydrogenases and other enzymes with affinity for NADP+ Using 2’5’ ADP Sepharose 4B and Red Sepharose CL-6B
This page shows how to purify NADP+-dependent dehydrogenases and other enzymes with affinity for NADP+ by affinity chromatography using 2’5’ ADP Sepharose 4B and Red Sepharose CL-6B from Cytiva.
Capto Adhere
Capto adhere from Cytiva is a multimodal strong anion exchanger for BioProcess applications.
Performing High-Throughput Screening Using His MultiTrap™ HP and His MultiTrap™ FF 96-Well filter Plates
This page shows high-throughput screening using His MultiTrap™ HP and His MultiTrap™ FF 96-well filter plates from Cytiva.
Performing a Purification of IgG Antibodies with Protein G GraviTrap™
This page shows how to separate IgG antibodies by affinity chromatography using Protein G GraviTrap from Cytiva.
Manual Purification of Histidine-Tagged Proteins using His GraviTrap™ TALON® Columns
His GraviTrap™ TALON® columns are prepacked with 1 ml of TALON® Superflow medium. Each column provides simple manual purification of up to 15 mg of histidine-tagged proteins.

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