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Desalting and Buffer Exchange for Affinity Chromatography of Antibodies
Desalting at laboratory scale is a well-proven, simple, and fast method that will rapidly remove low molecular weight contaminants at the same time as transferring the sample into the desired buffer in a single step.
Maintenance of Multimodal Chromatography Media and Storage Conditions
This page describes the maintenance of media and storage conditions for multimodal chromatography using Cytiva products.
Column Packing and Preparation for Hydrophobic Interaction and Reversed Phased Chromatography
This page discusses column packing and preparation techniques for reverse phase chromatography.
Manual Purification of Histidine-Tagged Proteins using His GraviTrap™ TALON® Columns
His GraviTrap™ TALON® columns are prepacked with 1 ml of TALON® Superflow medium. Each column provides simple manual purification of up to 15 mg of histidine-tagged proteins.
Automated IgG Purification Using PureProteom
PureProteome™ Protein A and G Magnetic beads provide a rapid and reproducible means to purify immunoglobulins (IgG) using the KingFisher Duo particle processor.
Performing a Purification of NADP+-dependent dehydrogenases and other enzymes with affinity for NADP+ Using 2’5’ ADP Sepharose 4B and Red Sepharose CL-6B
This page shows how to purify NADP+-dependent dehydrogenases and other enzymes with affinity for NADP+ by affinity chromatography using 2’5’ ADP Sepharose 4B and Red Sepharose CL-6B from Cytiva.
RPC Purification Options and Scale up with SOURCE™ Media
The different stages of working with SOURCE™ (Cytiva) for high-resolution separation are discussed, from purification to polishing and scaling up
Anti-FLAG® M2 Magnetic Beads
Comparison of elution techniques for small-scale protein purification of FLAG® tag proteins using anti-FLAG® M2 magnetic beads.
Practical Considerations for IEX Separation
This page covers detailed aspects of each step in an IEX separation to improve resolution and overall performance.
Principles and Standard Conditions for Different Purification Techniques
This page provides information about the principles and standard conditions for different purification techniques for the purification of multiprotein complexes with from Cytiva.
Cleavage and Purification of GST-Tagged Protein Bound to Glutathione Sepharose in Batch Mode
This page shows how to cleave and purify GST-tagged proteins bound to Glutathione Sepharose in batch mode from Cytiva. Glutathione Sepharose High Performance, Glutathione Sepharose 4 Fast Flow, and Glutathione Sepharose 4B can all be used for cleavage and purification
Crystallization of Biomolecules
Many soluble proteins, membrane proteins, nucleic acids and nucleoprotein complexes have been obtained in a crystalline form suitable for crystallographic investigation.
Ion Exchange Chromatography Troubleshooting
This page covers practical problems that may lead to a non-ideal IEX separation and their solutions.
Troubleshooting Detection Methods for GST-tagged Proteins
This page describes troubleshooting strategies in the detection of GST-tagged proteins.
Chelators
BioUltra Chelators. A chelate is a water-soluble complex between a metal ion and a complexing agent. It usually does not dissociate easily in solution, but forms an inert complex.
Centrifugation-free Protein Extraction and Purification from Total Cultures of Baculovirus-infected Insect Cells
Centrifugation-free Protein Extraction and Purification from Total Cultures of Baculovirus-infected Insect Cells
Purifcation or Removal of Serine Proteases, e.g. Thrombin and Trypsin, and Zymogens
This page shows how to remove serine proteases, e.g. thrombin and trypsin, and zymogens from recombinant products using HiTrap Benzamidine FF and Benzamidine Sepharose 4 Fast Flow from Cytiva.
Characteristics of Dextrin Sepharose® High Performance Products
This page shows the characteristics of Dextrin Sepharose High Performance products from Cytiva.
Troubleshooting Methods for Purification of MBP-Tagged Recombinant Proteins
Troubleshooting of methods for purification of MBP-tagged recombinant proteins using Cytiva products.
Performing a Purification and On-column Refolding of an Insoluble Histidine-tagged Protein
This page shows how to perform a purification and on-column refolding of an insoluble his-tagged protein from an E. coli culture with HisTrap™ FF columns and ÄKTAprime from Cytiva.
The Potential of Dendritic Polyester Scaffolds as Biocompatible Drug Delivery Agents
Professor Michael Malkoch provides an overview of the unique properties of dendritic molecules and their application in biomedical fields. He highlights bis-MPA dendritic scaffolds as a promising biodegradable and biocompatible platform for drug delivery applications
General Considerations for Purification of GST-tagged Proteins
This page describes general considerations for purification of GST-tagged proteins using Cytiva products.
Organelle Isolation
The isolation of subcellular fractions by centrifugation is a commonly used technique and is widely applicable across multiple cell and tissue types. Because organelles differ in their size, shape, and density, centrifugation can be easily employed to separate and purify
Purification or Removal of Serine Proteases, such as Thrombin and Trypsin, and Zymogens
This page shows how to Purify or remove serine proteases with a Benzamidine Sepharose 4 Fast Flow, Benzamidine Sepharose 4 Fast Flow from Cytiva.
Performing a Separation with HiTrap® Protein G HP
Purify monoclonal or polyclonal IgG from serum, cell culture supernatant or ascitic fluid using the HiTrap Protein G HP from Cytiva, an affinity-exclusion chromatography product containing Sepharose-immobilized Protein G.
Capto Adhere Anion Exchanger for MAbs & Bind/Elute
Capto adhere from Cytiva is a multimodal strong anion exchanger for BioProcess applications.
Performing a Separation with IgG Sepharose 6 Fast Flow
Perform a separation with IgG Sepharose 6 Fast Flow from Cytiva, an Affinity Chromatography product for purification of recombinant fusion proteins containing a protein A tail.
Cleavage and Purification of GST-Tagged Protein Bound to GSTrap
This page shows how to cleave and purify GST-tagged proteins bound to GSTrap from Cytiva.
Troubleshooting Purification Methods
This page shows troubleshooting methods for purification of GST-tagged proteins using Cytiva products.
Manual and Automated Purification
This page describes manual and automated purification of histidine-tagged recombinant proteins using Cytiva products
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