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Studies of Dimerization and Activation of Receptors in the EGFR-Family Using Duolink® Assays
Our aim was to investigate the dimerization and activation pattern of two receptors, EGFR (ErbB1) and HER2 (ErbB2), in the EGFR-family using breast cancer cell lines and selected colon tissues as model systems.
Handling Storage
Handling & Storage of Custom Peptides
Duolink® Proximity Ligation Assay Instructional Video
This video will show you step-by-step how to perform a Duolink® PLA experiment with cells on a slide that have already been fixed and permeabilized by the user.
How to Optimize the Duolink® Proximity Ligation Assay
Things to consider for preparation, setup and execution of the Duolink® assay protocol
Detection of Protein-protein Interactions by in situ PLA
Proteins are complex biological molecules essential for cellular structure and functions. The majority of proteins commonly interact with various molecules, including other proteins, in order to exert their functions.
Absolute Quantification of Serum Proteome
Plasma proteomics holds great promise for the future of biomarker discovery, as well as <i>in vitro</i> diagnostics. Although plasma is readily accessible for analysis, the study of the plasma proteome is fundamentally limited by its vast dynamic range (10...
Duolink® Proximity Ligation Assay (PLA) Multicolor Kits for Sensitive Multiplex Detection
Duolink® PLA Multicolor technology and the quantification of protein-protein interactions in fixed tissues and cells.
Quantification of Infliximab in Human Serum by LC-MS/MS Using A Full-Length Stable Isotope Labeled Internal Standard
Infliximab, a chimeric monoclonal antibody, is used to treat rheumatoid arthritis, psoriatic arthritis, Crohn’s disease, and other autoimmune diseases.
How to Optimize Duolink® PLA for Flow Cytometry Detection
Considerations for proper experimental design, preparation and execution of the Duolink® PLA for flow cytometry protocol.
Troubleshooting 2-D DIGE Results
Troubleshooting 2-D DIGE Results
Single Molecule Detection with Atto 647N NTA
Significant improvement of single molecule tracking by using superior fluorescent NTA-Atto 647N conjugate
Duolink® PLA Flow Cytometry Kits
Traditional flow cytometry has been limited in the ability to detect protein-protein interactions and low abundant proteins events — until now. We have combined Duolink® Proximity Ligation Assay (PLA) with flow cytometry in a convenient kit, making the analysis of...
Peptide Sample Amount Determination
Determining the amount of desired product in a peptide sample.
Duolink® PLA Technology for Neuroscience Applications
Duolink® proximity ligation assay used to study neuron interactions furthering neuroscience research.
Duolink® PLA – Protein Detection Technology
Duolink based on Proximity Ligation Assay (in situ PLA) technology when combined with qualified antibodies outperforms traditional immunoassays, such as co-IP, through a simple and sensitive in situ detection of endogenous proteins. This technology enables you to visualize protein interactions...
Mass Spectrometry
What is Mass Spectrometry or MS? What is it used for and how does it work? Read this detailed article with diagrams and example graphs to understand how to use mass spec in your research.
AQUA™ Peptides for Absolute Quantitation
AQUA Protein - Ordering Page
Quantification of Host Cell Proteins
Residual presence of host cell proteins (HCPs) in recombinant therapeutic products has considerable clinical safety risks associated with a potential immunological response in patients.
Peptide Modifications: N-Terminal, Internal, and C-Terminal
Peptide Modifications: N-Terminal, Internal, and C-Terminal
Multiplex Quantification of Infliximab and Adalimumab in Human Serum by LC-MS/MS Using Full-Length Stable Isotope Labeled Internal Standards
Infliximab and Adalimumab monoclonal antibodies (mAbs) are used widely to treat rheumatoid arthritis, psoriatic arthritis, and many autoimmune diseases by binding to tumor necrosis factor-alpha (TNFα) to reduce the inflammatory response.
Direct Detect® Infrared Spectrometer for Total Protein Quantitation
Learn how total protein quantitation with an infrared spectrometer may be a more accurate and universal alternative to Bradford, BCA, or UV-based methods.
Duolink® References
Find Duolink references based on the type of method used, post translational modification detected, and research focus.
Troubleshooting Guide for Duolink® PLA for Flow Cytometry Detection
General tips and tricks for proper experiment execution, aid in identifying potential problems, and provide solutions to ensure a successful Duolink® PLA experiment for flow cytometry.
SMC™ Technology
Originally developed by Singulex®, Inc., Single Molecule Counting (SMC™) technology precisely quantifies molecules at previously undetectable levels.

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