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HPLC Analysis of Adenosine Monophosphate (AMP) and Adenosine Triphosphate (ATP) on SeQuant ZIC-HILIC
Separation of AMP (adenosine monophosphate); ATP (adenosine triphosphate)
Determination of Formaldehyde and Acetaldehyde in Air Using DNPH Cartridges and Automated On-Line Desorption Followed by HPLC
Carbonyl compounds react within the DNPH cartridge to form immobilized hydrazones, which can be subjected to automated desorption and HPLC analysis with UV detection.
Cannabinoid Certified Reference Materials (CRMs) for an Improved Testing Accuracy and Traceability
We offer a portfolio of cannabinoid CRMs such as THC and THCA, available as single component solutions or mixes, certified in accordance with ISO 17034 and ISO/IEC 17025 for accurate cannabinoid profiling and potency testing.
Chiral and Achiral LC-MS Analysis of Warfarin™ in Plasma Samples
Clinical laboratories are beginning to reap the benefits of LC-MS in terms of its sensitivity, specificity, throughput, and potential to reduce cost per sample. The purpose of this study was to demonstrate the LC-MS analysis of Warfarin in plasma samples
SupraSolv® GC Solvent Specifications
Our GC solvents have been specially developed and tested for ECD, FID and MS detection or for headspace gas chromatography to ensure accurate, reliable and reproducible analytical results.
Supel™-Select Polymeric SPE
Supel-Select HLB SPE - a hydrophilic modified styrene based polymer developed for the solid phase extraction of a broad range of compounds from aqueous samples.
Ion-Suppression & Phospholipid Contamination
We are presenting an article focusing on ion-suppression and phospholipid contamination and some of their major causes and difficulties.
Rapid Determination of Protein Binding Affinity Using Solid Phase Microextraction
Accurate protein binding affinities were measured using bioSPME more rapidly than membrane dialysis (RED) techniques. Binding affinities correlated to the reference values.
Bioanalysis with SPME
Biocompatible SPME devices employ C18 bonded porous silica sorbent particles, in a binder which resists fouling by biological matrix components
Determination of Ethyl Glucuronide and Ethyl Sulfate in Urine using LC-MS/MS
This study is aimed at developing and validating an LC-ESI-ion trap-MS/MS method for identification and quantification of ethyl glucuronide (EtG) and ethyl sulfate (EtS) as ethanol biomarkers for urine analysis.
HPLC Products: The Best Choice for Any LC Instrument
Discover our comprehensive portfolio of High-performance liquid chromatography (HPLC) columns and consumables for your various and specialized U/HPLC based applications― 100% compatible with HPLC, UHPLC, or UPLC® systems from any vendor.
Ion Chromatography & Cation Suppression Straightforward Cation Analysis at Trace Levels
Ammonium determination in acidic solutions with Metrosep C Supp 2 column using the acidic binary IC eluent nitric acid/rubidium nitrate concentrate and the sodium bicarbonate/sodium carbonate suppressor regenerant.
Separation of Organic Acids with 100% aqueous mobile phase using an Ascentis® Express AQ-C18 HPLC Column
A new RP-HPLC method is proposed for the determination of organic acids in 100% aqueous mobile phase using an Ascentis® Express AQ-C18 column
Improving the Chromatographic Separation of DMB-Labeled Sialic Acids for the Comparison of Biosimilars to Reference Materials
A significantly improved HPLC-fluorescence method for DMB-NANA and -NGNA, and application of this method to compare 2 candidate biosimilar therapeutic proteins to their respective RMs.
HPLC Troubleshooting Guide
Identify and correct common HPLC problems to restore column performance. Get help with peak problems (peak splitting, peak tailing, peak fronting, etc.), retention time, purge noise, and loss of resolution with example chromatograms.
Derivatization and Separation of Aliphatic Amines
Derivatization of Aliphatic Amines - Results from the Supelco Ascentis Express column show higher peak resolution, and improved peak shapes than typical chromatograms.
HybridSPE-Phospholipid Technology
This Sigma-Aldrich article discusses how the HybridSPE-Phospholipid Technology works and how the phospholipids are removed.
Benefits of Ascentis Express Phenyl-Hexyl over Biphenyl for the Separation of Pain Management Opiates
Although both biphenyl and phenyl-hexyl phases can resolve these compounds, the former exhibits excellent peak shape and substantially less silanol-derived ion exchange activity.
UHPLC analysis of Benzoyl peroxide and degradation products on Purospher® STAR RP-18e Hibar® HR
UHPLC analysis of Benzoyl peroxide and degradation products on Purospher®STAR RP-18e Hibar® HR - Conditions & Description
HPLC Separation of Fluorinated Pharmaceutical Compounds on Supelco Ascentis Columns
Fluorinated compounds possess distinct characteristics that confer desirable physico-chemical properties compared to their non-fl uorinated analogs. Their abundance in pharmaceuticals, chemicals, consumer products and as environmental pollutants dictates the need for a reliable analytical method. Ascentis HPLC columns, by virtue
UHPLC Analysis of Vitamin D2 & D3 Metabolites and Epimers on Supel™ Carbon LC Columns
Ultra high-performance liquid chromatographic (UHPLC) separation of Vitamin D Metabolites― 3-epi-25-hydroxyvitamin D2 and 25-hydroxyvitamin D2, using Supel™ Carbon LC column with baseline separation, excellent peak shape, and sensitivity.
UHPLC Analysis of Paraquat and Diquat on Supel™ Carbon LC
Ultra-high performance liquid chromatographic (UHPLC) separation of polar herbicides, paraquat and diquat using SupelTM Carbon LC column with baseline resolution, excellent peak shape, and good lot-to-lot reproducibility.
Improving the Current USP Method for the Analysis of Lansoprazole Drug Substance Using HPLC Columns Based on Fused-Core® Particle Technology
Compendial methods from the USP (United States Pharmacopeia) are widely used in pharmaceutical drug product and raw materials testing. However, not all methods in the USP use modern technologies. In chromatographic methods, it is not uncommon that older brands of
Evaluation of Different Enzymes on Hydrolysis Efficiencies of Glucuronide Drug Metabolites in Urine
β-glucuronidase (GUS) enzymes are utilized to hydrolyze glucuronide (gluc) drug metabolites to the parent drug, facilitating analysis by LC-MS/MS.
Improving HILIC Mode Chromatography by Choosing the Proper Injection and Wash Solvent
Improving HILIC Mode Chromatography by Choosing the Proper Injection and Wash Solvent
Ascentis® Express Biphenyl UHPLC and HPLC Columns
The bonded, densely endcapped, dimethyl-biphenyl stationary phase of Ascentis® Express 90 Å Biphenyl, based on fused-core particles, provides a stable, reversed phase packing with enhanced π-π and mild steric interactions due to the two sequential phenyl groups bonded to the
Ultra-High Performance Liquid Chromatography (UHPLC) with the Ascentis® Express Phenyl-Hexyl Column
Ultra-High Performance Liquid Chromatography (UHPLC) with the Ascentis® Express Phenyl-Hexyl Column
Chromatographic Selectivity
Harnessing the Power of Chromatographic Selectivity
Developing HPLC Methods
We recommend every HPLC method developer have these four columns in their arsenal; Ascentis C18, Ascentis RP-Amide, Ascentis Phenyl and Discovery HS F5.
HPLC Analysis of Xanthines on Purospher® STAR RP-18 endcapped
Xanthine is a purine base found in most human body tissues and fluids as well as in other organisms. Methylated xanthines (methylxanthines), which include caffeine, paraxanthine, theobromine, and theophylline, commonly used for their effects as mild stiµlants and as bronchodilators
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