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How to Run Milliplex
Key Steps in the MILLIPLEX® Assay Protocol for the Analysis of Soluble Biomarkers
Media Sterilization – Plant Tissue Culture Protocol
Plant tissue culture media are generally sterilized by autoclaving at 121 °C and 1.05 kg/cm2 (15-20 psi). The time required for sterilization depends upon the volume of medium in the vessel.
A Simple Strategy for Protein Enrichment Using Ultrafiltration
The human plasma proteome is a vast resource for biomarker discovery and investigation.
Antibody Drug Conjugate Mimic Enables LC-MS Method Development Without Risk
Here we show how LC and MS methods may be optimized using a non-toxic surrogate of the ADC, an “ADC-mimic”, that behaves very similarly to the Cys-linked ADC Adcetris (Seattle Genetics).
Enzymatic Method for Determining Urea (Phenylalanine Assay)
Assay protocol for the colorimetric detection of urea in biological samples using the urea assay kit.
Extracellular Vesicle Protocol
Best practices for standardizing and reducing the Extracellular Vesicle Preparation workflow, and eliminating contamination of EV preparations with dead-cell-derived vesicles.
Simple dialysis of Small Volume Samples using V Series Membranes
This article is a protocol for Simple dialysis of small volumes using V Series membranes
ELISA Procedures
ELISA protocols providing detailed instructions on performing indirect ELISA and Capture ELISA . Learn more about recommended products and techniques for performing both sandwich ELISA and indirect ELISA experiments.
Enzymatic Assay of 3α-Hydroxysteroid Dehydrogenase with Androsterone (EC 1.1.1.50)
Enzymatic Assay of 3α-Hydroxysteroid Dehydrogenase with Androsterone (EC 1.1.1.50)
Production of 15N-13C Dual-labelled Proteins
Dr. Alan Robertson and Dr. Ben Davis at Vernalis, Ltd., UK, a provider of drug discovery and development services, have refined a novel protocol for the duallabelling of proteins using EnPresso® B Defined Nitrogen-free.
Enzymatic Assay of Cellulase
To standardize an enzymatic assay procedure of cellulase.
Preparing the Plexcore® PV 1000 Ink System
Preparing the Plexcore® PV 1000 Ink System
Enzymatic Assay of Superoxide Dismutase
Enzymatic Assay of Superoxide Dismutase
Yeast Drop Out
Yeast Drop Out Bulletin. The selection of plasmids in yeast is based on the use of auxotrophic mutant strains, which cannot grow without a specific medium component (an amino acid, purine or pyrimidine). Transformation with a plasmid containing the mutated
Integration of Sigma® TransPlex® WTA and the Complete WTA2 Kits with the NimbleGen™ Expression Microarray Workflow
Amplification products generated by the TransPlex® WTA and Complete WTA2 kits are suitable for microarray target for expression analyses, and can be readily integrated into existing NimbleGen workflows.
LPL Activity Assay Protocol
Lipoprotein lipase (LPL) hydrolyzes triglycerides associated with VLDL.
Coupling to Microparticles
Biochemicals Coupling to Microparticles. Besides Proteins, DNA, lectins and many other species have been coupled to microparticles.
Enzymatic Assay of Ficin (EC 3.4.22.3)
This procedure may be used for all Ficin products.
Assay Procedure for Xanthine Oxidase Microbial
The appearance of uric acid is measured at 293nm by spectrophotometry.
Enzymatic Assay of Endoproteinase Glu-C (EC 3.4.21.19)
This procedure may be used for determination of Endoproteinase Glu-C enzymatic activity using N-t-Boc-L-glutamic acid α-phenyl ester as the substrate.
LCAT Activity Assay Kit
The LCAT Activity Assay Kit is a fluorometric assay useful for measuring phospholipase activity of LCAT.
Enzymatic Assay of Plasmin with D-Val-Leu-Lys-p-Nitroanilide Dihydrochloride
Enzymatic Assay of Plasmin with D-Val-Leu-Lys-p-Nitroanilide Dihydrochloride
Immunoprecipitation Kit (Protein A) Protocol & Troubleshooting
Immunoprecipitation Kit (Protein A) Protocol & Troubleshooting
Imprint® Chromatin Immunoprecipitation Kit (CHP1) Protocol
The Sigma Imprint Chromatin Immunoprecipitation Kit uses a plate based system to allow rapid ChIP assays in a high throughput format
Liberase™ DH Research Grade Protocol & Troubleshooting
Your current tissue dissociation protocol, and the extent to which you have been successful with traditional collagenase are good guides for selecting the appropriate Liberase Enzyme Blend.
Ion Channel Flux Assays and Protocols
A review and resources for ion channel flux assays and protocols, along with liposome formation and proteoliposome preparation protocols
Immunoprecipitation Procedure
To determine the molecular weights of protein antigens, to study protein/protein interactions, to determine specific enzymatic activity, to monitor protein post-translational modifications and to determine the presence and quantity of proteins.
Use of the Protease Fluorescent Detection Kit to Determine Protease Activity
The Protease Fluorescent Detection Kit provides ready-to-use reagents for detecting the presence of protease activity. This simple assay to detect protease activity uses casein labeled with fluorescein isothiocyanate (FITC) as the substrate.
Assay Procedure for Cholesterol Oxidase
Protocol for Assay Procedure for Cholesterol Oxidase
Dextran Sulfate
Dextran sulfates are supplied as the sodium salt forms, making them soluble and stable in water. Dextran sulfate contains approximately 17% sulfur which is equivalent to approximately 2.3 sulfate groups per glucosyl residue.
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