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Dynamic Live Cell Imaging of Caspase Mediated Apoptosis and Cellular Hypoxia in Lymphocytes and Cancer Cells Using the CellASIC® ONIX2 Microfluidic Platform
Application note on how the CellASIC® ONIX2 microfluidic system can be used to analyze caspase-3 mediated apoptosis/cell death and cellular hypoxia in live immune and cancer cell lines.
Materials for Thin Film Solid Oxide Fuel Cells (TF-SOFCs)
This technical spotlight focuses on the thin film solid oxide fuel cells (TF-SOFC). TF-SOFCs refer to solid oxide fuel cells with electrolyte thin films. This review highlights technical advantages of TF-SOFC and introduces deposition methods of electrolyte thin films to
Recent Developments in Silicon Anode Materials for High Performance Lithium-Ion Batteries
Recent demand for electric and hybrid vehicles, coupled with a reduction in prices, has caused lithium-ion batteries (LIBs) to become an increasingly popular form of rechargeable battery technology.
Advancements in Quantum Dot Solar Cells
Professor Sharma and colleagues review the synthesis and applications of this novel material. This includes a discussion of the unique properties of quantum dots and their suitability for solar cell applications, along with common synthesis techniques used to develop these
Vibrio Detection
Vibrios are motile, curved or comma-shaped bacilli and have a single polar flagella with sheet proteins. They are often found in open water, freshwater and saltwater.
Surface-enhanced Solar Energy Conversion Systems Using Gold and Silver Nanoparticles
Sustainable, environment-friendly, and clean energy sources with sufficiently high production efficiency for practical application are highly desirable to meet the energy challenge of the 21st century due to the world′s increasing energy demand.
Olivine-Type Cathode Materials for Lithium-Ion Batteries
Lithium-ion batteries (LIBs) have been widely adopted as the most promising portable energy source in electronic devices because of their high working voltage, high energy density, and good cyclic performance.
Detection, identification, differentiation and cultivation of Yersinia species
Detection, identification, differentiation and cultivation of Yersinia species
Drug transport assays in a 96-well system: reproducibility and correlation to human absorption
Application note on Drug transport assays in a 96-well system using Millicell-96 System from Millipore.
Inhibition of Nucleic Acid Synthesis by Antibiotics
Quinolones are a key group of antibiotics that interfere with DNA synthesis by inhibiting topoisomerase, most frequently topoisomerase II (DNA gyrase), an enzyme involved in DNA replication.
Actin
Although actin is one of the most conserved eukaryotic proteins, it is expressed as six isoforms characterized by electrophoresis and amino acid sequence analysis. Four of the six isoforms represent differentiation markers of muscle tissues. The other two are found
Handling and Storage Guidelines for Peptides and Proteins
Handling and Storage Guidelines for Peptides and Proteins
Nanodisc Technology: A Revolutionary System for Study of Membrane Proteins
Read our article about how the Nanodisc system allows for structural studies of membrane proteins.
Folic Acid Metabolism in Cancer
This issue of Biofiles reviews some of our newest and most innovative technologies and their specific applications toward cancer research. In preparing this issue of Biofiles, one is reminded how complex the disease of cancer is, and how difficult it
Interleukin Family (IL)
The Interleukins comprise a disparate group of cytokines and growth factors that are produced by and released from leukocytes. Interleukin-1β (1L-1β) is released primarily from stimulated macrophages and monocytes and plays a key role in inflammatory and immune responses and
DNA Damage and Repair
DNA damage and repair mechanism is vital for maintaining DNA integrity. Damage to cellular DNA is involved in mutagenesis, the development of cancer among others.
Troponin
Troponin together with tropomyosin, regulate the binding of myosin to actin. Troponin is a trimeric protein composed of Troponin subunits I, C and T. Troponin C binds calcium ions, Troponin T binds to tropomyosin and troponin I binds
Cholesterol Biosynthesis Regulation
The amount of cholesterol that is synthesized in the liver is tightly regulated by dietary cholesterol levels. LDL receptors regulate the cellular transport of lipid rich low density lipoprotein (LDL) particles.
Robust Differentiation of Human iPSCs into Lineage-Specific Neurons and Glial Cells Utilizing Dual-SMAD Inhibition
Human iPSC neural differentiation media and protocols used to generate neural stem cells, neurons and glial cell types.
Combining Permeability and Solubility Analyses in a Single Workflow
This article details the benefits of combining solubility and a non-cell based permeability assay (Parallel Artificial Membrane Permeation Assay; PAMPA) into a single workflow in which a portion of the filtrate from the MultiScreen® Solubility filter plate is added to
Phase I Drug Metabolism
Phase I biotransformation reactions introduce or expose functional groups on the drug with the goal of increasing the polarity of the compound. Although Phase I drug metabolism occurs in most tissues, the primary and first pass site of metabolism occurs
Properties of Mononuclear Cells Isolated by the Ficoll-Paque Method
The effects of the mononuclear cell separation method described by Bøyum procedure are noted below, since research situations may arise in which they are significant.
Vimentin
Vimentin is an intermediate filamentous cytosketetal protein found in cells of mesenchymal origin. Vimentin typically exists as a coiled-coil dimer that interacts with another dimers to form a tetrameric complex that forms cytoskeletal protein sheets
Membrane Integrity Test for Lipid-PAMPA Artificial Membranes
This is a protocol for a Membrane Integrity test for Lipid-PAMPA Artificial Membranes
Cell Counting Using a Hemocytometer
Cell counting protocol using a hemocytometer and trypan blue for measuring cell viability.
Biotinylation Reagents
Biotin-labelled peptides have many important applications in immunology and histochemistry, such as affinity purification and FRET-based flow cytometry, solid-phase immunoassays, and receptor localization, that exploit the high affinity of streptavidin and avidin for biotin.
Transferrin in Cell Culture
How transferrin and other cell culture components affect the performance of serum-free, protein-free cell culture systems used for biomanufacturing heterologous proteins including monoclonal antibodies. The page introduces the in vitro chemistry and biochemistry of transferrin.
Linolenic Acid in Cell Culture
Importance and uses of linolenic acid in serum-free eukaryotic, including hybridoma and Chinese Hamster Ovary (CHO) cell, cultures
Poor Adherent Cell Attachment Troubleshooting
Cells that are cultured under suboptimal growth conditions attach poorly. When the culture surface provided is appropriate, the most common cause of failure of cells to attach to a substrate is environmental stress placed on the cells.
Hepatocyte Growth Factor (HGF)
Hepatocyte growth factor (HGF) is a potent mitogen for hepatocytes and a variety of other cells, including endothelial and epithelial cells, melanocytes, and keratinocytes.
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