Showing 1-30 of 81 results for "G8877"
Samuel T Kissinger et al.
Cell reports, 31(1), 107486-107486 (2020-04-09)
Fragile X syndrome (FX), the most common inherited form of autism and intellectual disability, is a condition associated with visual perceptual learning deficits. We recently discovered that perceptual experience can encode visual familiarity via persistent low-frequency oscillations in the mouse...
Georg Hafner et al.
Cell reports, 28(13), 3450-3461 (2019-09-26)
Parvalbumin (PV)-expressing GABAergic neurons are the largest class of inhibitory neocortical cells. We visualize brain-wide, monosynaptic inputs to PV neurons in mouse barrel cortex. We develop intersectional rabies virus tracing to specifically target GABAergic PV cells and exclude a small...
A L Menon et al.
Journal of bacteriology, 176(2), 291-295 (1994-01-01)
H2 oxidation in Azotobacter vinelandii is catalyzed by a membrane-bound, alpha beta dimeric [NiFe] hydrogenase. Maturation of the enzyme involves cleavage of a putative N-terminal signal sequence in the beta subunit and removal of 15 amino acids from the C...
Lei Zhang et al.
Nature communications, 11(1), 5371-5371 (2020-10-25)
Autoinducer-2 (AI-2) is a quorum sensing signal that mediates communication within and between many bacterial species. However, its known receptors (LuxP and LsrB families) are not found in all the bacteria capable of responding to this signaling molecule. Here, we...
Kuang Shen et al.
Cell, 179(6), 1319-1329 (2019-11-11)
mTORC1 controls anabolic and catabolic processes in response to nutrients through the Rag GTPase heterodimer, which is regulated by multiple upstream protein complexes. One such regulator, FLCN-FNIP2, is a GTPase activating protein (GAP) for RagC/D, but despite its important role...
S P Gilbert et al.
Biochemistry, 32(17), 4677-4684 (1993-05-04)
The Drosophila kinesin heavy-chain gene was truncated to obtain the N-terminal 401 amino acid motor domain (designated K401) containing both the microtubule and ATP binding sites. The plasmid construct with the truncated kinesin gene was used to transform Escherichia coli....
Ines Leca et al.
PLoS genetics, 16(11), e1009104-e1009104 (2020-11-03)
Microtubules play a critical role in multiple aspects of neurodevelopment, including the generation, migration and differentiation of neurons. A recurrent mutation (R402H) in the α-tubulin gene TUBA1A is known to cause lissencephaly with cerebellar and striatal phenotypes. Previous work has...
J E Geiger et al.
Neuroscience, 162(4), 1023-1038 (2009-05-12)
Ca(2+) influx through voltage-gated Ca(2+) channels is a fundamental signaling event in neurons; however, non-traditional routes, such as non-selective cation channels, also permit Ca(2+) entry. The present study examines the Ca(2+) permeability of a cation channel that drives an afterdischarge...
Marta Pabis et al.
The EMBO journal, 39(19), e105087-e105087 (2020-09-10)
The chemical modification of tRNA bases by sulfur is crucial to tune translation and to optimize protein synthesis. In eukaryotes, the ubiquitin-related modifier 1 (Urm1) pathway is responsible for the synthesis of 2-thiolated wobble uridine (U34 ). During the key...
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Jonathan A Winger et al.
Biochemistry, 44(10), 4083-4090 (2005-03-09)
The catalytic domains (alpha(cat) and beta(cat)) of alpha1beta1 soluble guanylate cyclase (sGC) were expressed in Escherichia coli and purified to homogeneity. alpha(cat), beta(cat), and the alpha(cat)beta(cat) heterodimeric complex were characterized by analytical gel filtration and circular dichroism spectroscopy, and activity...
Yang Du et al.
Cell, 177(5), 1232-1242 (2019-05-14)
The activation of G proteins by G protein-coupled receptors (GPCRs) underlies the majority of transmembrane signaling by hormones and neurotransmitters. Recent structures of GPCR-G protein complexes obtained by crystallography and cryoelectron microscopy (cryo-EM) reveal similar interactions between GPCRs and the...
Sang Hun Lee et al.
Neuron, 108(4), 676-690 (2020-09-07)
Amyloid precursor protein (APP) is associated with both familial and sporadic forms of Alzheimer's disease. Despite its importance, the role of APP family in neuronal function and survival remains unclear because of perinatal lethality exhibited by knockout mice lacking all...
Noemi Holderith et al.
Cell reports, 32(4), 107968-107968 (2020-07-30)
Elucidating the molecular mechanisms underlying the functional diversity of synapses requires a high-resolution, sensitive, diffusion-free, quantitative localization method that allows the determination of many proteins in functionally characterized individual synapses. Array tomography permits the quantitative analysis of single synapses but...
Andrea F Jones et al.
Cell reports, 31(10), 107729-107729 (2020-06-11)
The medial prefrontal cortex (mPFC) modulates a range of behaviors, including responses to noxious stimuli. While various pain modalities alter mPFC function, our understanding of changes to specific cell types underlying pain-induced mPFC dysfunction remains incomplete. Proper activity of cortical...
P H Hünenberger et al.
Biochemistry, 38(8), 2358-2366 (1999-02-25)
Protein kinases are essential for the regulation of cellular growth and metabolism. Since their dysfunction leads to debilitating diseases, they represent key targets for pharmaceutical research. The rational design of kinase inhibitors requires an understanding of the determinants of ligand...
P Barbier et al.
Biochemistry, 37(2), 758-768 (1998-02-21)
NSC 613862 (S)-(-) and NSC 613863 (R)-(+) are the two chiral isomers of ethyl-5-amino-2-methyl-1,2-dihydro-3-phenylpyrido[3, 4-b]pyrazin-7-yl carbamate. Both compounds bind to tubulin in a region that overlaps the colchicine site. They induce formation of abnormal polymers from purified GTP-Mg-tubulin, the active...
Quan Wang et al.
Cell, 182(2), 417-428 (2020-06-12)
Nucleotide analog inhibitors, including broad-spectrum remdesivir and favipiravir, have shown promise in in vitro assays and some clinical studies for COVID-19 treatment, this despite an incomplete mechanistic understanding of the viral RNA-dependent RNA polymerase nsp12 drug interactions. Here, we examine the...
Flufenamic acid affects multiple currents and causes intracellular Ca2+ release in Aplysia bag cell neurons
Gardam K E, et al.
Journal of Neurophysiology, 100(1), 38-49 (2008)
Xueqin Wang et al.
Neurobiology of stress, 13, 100240-100240 (2020-12-22)
Gulf War illness is associated with a combination of exposure to war-related chemical agents and traumatic stress. Currently, there are no effective treatments, and the pathophysiology remains elusive. Neurological problems are among the most commonly reported symptoms. In this study...
Shuo Jiang et al.
Developmental cell, 49(5), 711-730 (2019-04-30)
The correct localization of Hedgehog effectors to the tip of primary cilia is critical for proper signal transduction. The conserved non-motile kinesin Kif7 defines a "cilium-tip compartment" by localizing to the distal ends of axonemal microtubules. How Kif7 recognizes microtubule ends...
J Spychala et al.
The International journal of biochemistry, 23(10), 1155-1159 (1991-01-01)
1. Phosphocellulose column chromatography under double gradient conditions (phosphate and KCl) revealed two forms of AMP deaminase in rat heart and brain and a single form in the liver and skeletal muscle. 2. Kinetically all purified AMP deaminases were classified...
Jiefei Wang et al.
Cell chemical biology, 26(5), 745-755 (2019-03-25)
Calprotectin (CP) inhibits bacterial viability through extracellular chelation of transition metals. However, how CP influences general metabolism remains largely unexplored. We show here that CP restricts bioavailable Zn and Fe to the pathogen Acinetobacter baumannii, inducing an extensive multi-metal perturbation...
Max A Horlbeck et al.
Cell, 174(4), 953-967 (2018-07-24)
Seminal yeast studies have established the value of comprehensively mapping genetic interactions (GIs) for inferring gene function. Efforts in human cells using focused gene sets underscore the utility of this approach, but the feasibility of generating large-scale, diverse human GI...
Christopher J Groten et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 35(6), 2747-2765 (2015-02-13)
It is unknown whether neurons can dynamically control the capacity for secretion by promptly changing the number of plasma membrane voltage-gated Ca(2+) channels. To address this, we studied peptide release from the bag cell neurons of Aplysia californica, which initiate...
Mark Jelcic et al.
Cell chemical biology, 27(8), 1073-1083 (2020-06-11)
ATP is an important energy metabolite and allosteric signal in health and disease. ATP-interacting proteins, such as P2 receptors, control inflammation, cell death, migration, and wound healing. However, identification of allosteric ATP sites remains challenging, and our current inventory of...
Rob P M van Gijlswijk et al.
Clinical chemistry, 48(8), 1352-1359 (2002-07-27)
Microarray platforms will change immunochemical and nucleic acid-based analysis of cell homogenates and body fluids compared with classic analyses. Microarrays use labeled target and immobilized probes, rather than fixed targets and labeled probes. We describe a method for simultaneous labeling...
Amol Aher et al.
Current biology : CB, 30(11), 2175-2183 (2020-05-04)
Microtubules play a key role in cell division, motility, and intracellular trafficking. Microtubule lattices are generally regarded as stable structures that undergo turnover through dynamic instability of their ends [1]. However, recent evidence suggests that microtubules also exchange tubulin dimers...
Birgit Dreier et al.
Methods in molecular biology (Clifton, N.J.), 687, 283-306 (2010-10-23)
The selection and concomitant affinity maturation of proteins to bind to user-defined target molecules have become a key technology in biochemical research, diagnostics, and therapy. One of the most potent selection technologies for such applications is ribosome display. It works...
A Margulis et al.
Biochemistry, 39(5), 1034-1039 (2000-02-02)
Soluble guanylate cyclase (sGC) is highly activated by nitric oxide (NO) and is the known mediator of the effects of NO on a variety of physiological processes. The rates at which sGC is activated and deactivated are therefore of wide...

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