Applications
Content Type
Collections
Showing 1-30 of 78 results for "Glycine"
Hand-casting gels for PAGE and SDS-PAGE using TurboMix™ Bis-Tris Gel Casting Kits
Overview of polyacrylamide gel chemistry and detailed instructions for hand-casting polyacrylamide gels using TurboMix™ Bis-Tris Gel Casting Kits
Enzymatic Assay of β-Glucuronidase (EC 3.2.1.31) from E. coli
Objective: To standardize a procedure for the enzymatic assay of β-Glucuronidase.
Enzymatic Assay and ATP Sensitivity Test of Luciferase
Objective: To standardize a procedure to determine the enzymatic activity of Luciferase and/or to determine the ATP detection limit of Luciferase.
Enzymatic Assay of Choloylglycine Hydrolase
To standardize a procedure for the determination of the enzymatic assay of choloylglycine hydrolase.
Enzymatic Assay of ß-Glucuronidase (EC 3.2.1.31) from Helix Pomatia and Bovine Liver
Enzymatic Assay of ß-Glucuronidase (EC 3.2.1.31) from Helix Pomatia and Bovine Liver
Rehydration Solutions
This page describes typical rehydration solutions for use in gel electrophoresis with Cytiva products.
Trypsin Inhibitors
Natural trypsin Inhibitors also known as serine protease inhibitors (serpins) are the largest and most diverse family of protease inhibitors. Serpins control the activation and catabolism of proteins by the inhibition of serine proteases in vivo.
Enzymatic Method for Determining L-Amino Acids (L-Amino Acid Quantitation Assay)
Assay protocol for the colorimetric/fluorometric detection of L-Amino Acids in biological samples using the L-Amino Acid assay kit
Fast and Robust HPLC Separation of Bile Acids and Conjugates with Ascentis Express C18
This method is particularly useful in research into the role of individual bile acids as signaling molecules; suitable for clinical laboratories to investigate potential mechanisms linked to gut hormone profiles and glycemic control.
Immunoprecipitation of FLAG Fusion Proteins Using Monoclonal Antibody Affinity Gels
Protocol for immunoprecipitation (IP) of FLAG fusion proteins using M2 monoclonal antibody 4% agarose affinity gels
Enzymatic Assay of Phytase (EC 3.1.3.8)
Derived from procedure SSPHYT02. Includes template updates to current SOP specifications and incorporation of notes into the procedure.
Sera-Mag™ SpeedBeads Magnetic Protein A/G-Particles Protocol
Sera-Mag SpeedBeads Protein A/G Magnetic Particles provide a fast and convenient method for both manual and automated magnetic isolation of proteins using affinity binding. The particles can be used for isolating antibodies from serum, cell culture supernatant or ascites, and...
Affinity Purification with Ultrafree®-MC Centrifugal Filters
Affinity interaction chromatography is an effective method for protein purification that can achieve up to 95% purity in one step
Preactivated Magnetic Beads for Affinity Chromatography
Covalent coupling of antibodies, aptamers, and proteins with a NHS Mag Sepharose, Sera-Mag Carboxylate-Modified, Sera-Mag SpeedBeads Carboxylate-Modified from Cytiva.
Purification or Removal of Albumin and IgG
This page shows how to purify or remove albumin and IgG with Albumin & IgG Depletion Sepharose High Performance from Cytiva.
GC Analysis of Amino Acids (as TBDMS Derivatives) on SLB®-5ms (20 m x 0.18 mm I.D., 0.18 μm), Fast GC Analysis
Separation of L-Alanine; Glycine; L-Valine; L-Leucine; L-Isoleucine; L-Proline; L-Methionine; L-Serine; L-Threonine; L-Phenylalanine; L-Aspartic acid; L-4-Hydroxyproline; L-Cysteine; L-Glutamic acid; L-Asparagine; L-Lysine; L-Glutamine; L-Histidine; L-Tyrosine; L-Tryptophan; L-Cystine
Purification or Removal of Biotin and Biotinylated Substances
This page shows how to purify or remove biotin and biotinylated substances with Streptavidin Sepharose High Performance from Cytiva.
Spermine Tetrahydrochloride for Molecular Biology
This product is suitable for DNA precipitation from low salt aqueous buffers. It has been tested for the absence of proteases and nucleases.
Enzymatic Assay of 5’-Nucleotidase
Objective: To standardize a procedure for determining the enzymatic activity of 5’-Nucleotidase.
Two-step Purification of Mouse Monoclonal IgG1 Using HiTrap® Protein G HP for the Capture Step
This page shows how to perform a two-step purification of mouse monoclonal IgG1 using HiTrap® Protein G HP from Cytiva for the capture step.
Imprint® Chromatin Immunoprecipitation Kit (CHP1) Protocol
The Sigma Imprint Chromatin Immunoprecipitation Kit uses a plate based system to allow rapid ChIP assays in a high throughput format
Vitamin Mixtures – Plant Tissue Culture Protocol
Preparation of Solutions from Packaged Powder, Media Preparation using Vitamin Solutions, Vitamin Mixtures Composition Table
Coupling to Microparticles
Biochemicals Coupling to Microparticles. Besides Proteins, DNA, lectins and many other species have been coupled to microparticles.
Performing a Purification of IgG Antibodies with Protein G GraviTrap™
This page shows how to separate IgG antibodies by affinity chromatography using Protein G GraviTrap from Cytiva.
Performing a Separation with Cytiva Products Based on Protein A
Protein A is derived from a strain of Staphylococcus aureus and contains five regions that bind to the Fc region of IgG. As an affinity ligand, protein A is coupled to Sepharose so that these regions are free to bind...
Purification or Removal of Biotin and Biotinylated Biomolecules with Magnetic Beads
This page shows how to Purify or remove biotin and biotinylated biomolecules with magnetic beads.
Two-Dimensional Separation of Protein Samples
This page describes two-dimensional separation of protein samples with Cytiva products.
Diethanolamine Assay for the Enzymatic Assay of Alkaline Phosphatase (EC 3.1.3.1)
Enzymatic Assay of Alkaline Phosphatase, Diethanolamine Assay (EC 3. 1. 3. 1)
Purification of Protein A-Tagged Proteins
This page shows how to purify protein A-tagged proteins using Cytiva products.
Yeast Drop Out
Yeast Drop Out Bulletin. The selection of plasmids in yeast is based on the use of auxotrophic mutant strains, which cannot grow without a specific medium component (an amino acid, purine or pyrimidine). Transformation with a plasmid containing the mutated...

Social Media

LinkedIn icon
Twitter icon
Facebook Icon
Instagram Icon

MilliporeSigma

Research. Development. Production.

We are a leading supplier to the global Life Science industry with solutions and services for research, biotechnology development and production, and pharmaceutical drug therapy development and production.

© 2021 Merck KGaA, Darmstadt, Germany and/or its affiliates. All Rights Reserved.

Reproduction of any materials from the site is strictly forbidden without permission.