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Dong Jae Kim et al.
Small (Weinheim an der Bergstrasse, Germany), 14(40), e1802520-e1802520 (2018-08-22)
Surface-enhanced Raman scattering (SERS) provides a dramatic increase of Raman intensity for molecules adsorbed on nanogap-rich metal nanostructures, serving as a promising tool for molecular analysis. However, surface contamination caused by protein adsorption and low surface concentration of small target
Active suppression of early immune response in tobacco by the human pathogen Salmonella Typhimurium.
Natali Shirron et al.
PloS one, 6(4), e18855-e18855 (2011-05-05)
The persistence of enteric pathogens on plants has been studied extensively, mainly due to the potential hazard of human pathogens such as Salmonella enterica being able to invade and survive in/on plants. Factors involved in the interactions between enteric bacteria
Myles Sedgwick et al.
Journal of the American Chemical Society, 134(29), 11904-11907 (2012-07-07)
The dramatic impact of differing environments on proton transfer dynamics of the photoacid HPTS prompted us to investigate these systems with two highly complementary methods: ultrafast time-resolved transient absorption and two-dimensional NMR spectroscopies. Both ultrafast time-resolved transient absorption spectroscopy and
Wei-Hai Chen et al.
ACS nano, 12(8), 7538-7545 (2018-07-04)
Zeolitic Zn2+-imidazolate cross-linked framework nanoparticles, ZIF-8 NMOFs, are used as "smart" glucose-responsive carriers for the controlled release of drugs. The ZIF-8 NMOFs are loaded with the respective drug and glucose oxidase (GOx), and the GOx-mediated aerobic oxidation of glucose yields
Boiko Cohen et al.
The journal of physical chemistry. B, 115(23), 7637-7647 (2011-04-12)
We report on femto- to nanosecond studies of the excited state intermolecular proton transfer (ESPT) reaction of trisodium 8-hydroxypyrene-1,3,6-trisulfonate (pyranine, HPTS) with the human serum albumin (HSA) protein. The formed robust 1:1 complexes (K(eq) = (2.6 ± 0.1) × 10(6)
Ye Yang et al.
Advanced materials (Deerfield Beach, Fla.), 27(32), 4725-4731 (2015-07-17)
A dynamically adjustable colloidal assembly technique is presented, which combines magnetic and acoustic fields to produce a wide range of colloidal structures, ranging from discrete colloidal molecules, to polymer networks and crystals. The structures can be stabilized and dried, making
Synthesis of p-and n-type Gels Doped with Ionic Charge Carriers.
Alveroglu E and Yilmaz Y
Nanoscale Research Letters, 5(3), 559-559 (2010)
Mayuko Morita et al.
Free radical research, 53(11-12), 1101-1113 (2019-11-07)
The unregulated oxidative modification of biological molecules has been implicated in the pathogenesis of various diseases, and the beneficial effects of antioxidants against detrimental oxidation have received much attention. Among the multiple oxidants, peroxyl radical and peroxynitrite play an important
Simon Lee et al.
Molecular therapy. Methods & clinical development, 7, 60-73 (2017-10-17)
New methods to produce large numbers of myeloid progenitor cells, precursors to macrophages (MΦs), by maintaining Hoxb8 transcription factor activity
Xi-Hui Yu et al.
Organic & biomolecular chemistry, 17(6), 1558-1571 (2019-01-30)
Anion transporters have attracted substantial interest due to their ability to induce cell apoptosis by disrupting cellular anion homeostasis. In this paper we describe the synthesis, anion recognition, transmembrane anion transport and cell apoptosis-inducing activity of a series of fluorinated
Yuri N Antonenko et al.
Biochimica et biophysica acta, 1848(4), 995-1004 (2015-01-21)
Monensin is a carrier of cations through lipid membranes capable of exchanging sodium (potassium) cations for protons by an electroneutral mechanism, whereas its ethyl ester derivative ethyl-monensin is supposed to transport sodium (potassium) cations in an electrogenic manner. To elucidate
Joseph E Thomaz et al.
The journal of physical chemistry. B, 121(17), 4544-4553 (2017-04-12)
Proton transfer in the nanoscopic water channels of polyelectrolyte fuel cell membranes was studied using a photoacid, 8-hydroxypyrene-1,3,6-trisulfonic acid sodium salt (HPTS), in the channels. The local environment of the probe was determined using 8-methoxypyrene-1,3,6-trisulfonic acid sodium salt (MPTS), which
Sadat Hasan et al.
Lab on a chip, 19(3), 403-409 (2019-01-04)
We present a highly efficient microfluidic fluorescence lifetime-activated droplet sorting (FLADS) approach as a novel technology for droplet manipulation in lab-on-a-chip devices. In a proof-of-concept study, we successfully applied the approach to sort droplets containing two different fluorescent compounds on
Functional importance of the oligomer formation of the cyanobacterial H+ pump Gloeobacter rhodopsin.
Azusa Iizuka et al.
Scientific reports, 9(1), 10711-10711 (2019-07-26)
Many microbial rhodopsins self-oligomerize, but the functional consequences of oligomerization have not been well clarified. We examined the effects of oligomerization of a H+ pump, Gloeobacter rhodopsin (GR), by using nanodisc containing trimeric and monomeric GR. The monomerization did not
Gurdeep S Minhas et al.
eLife, 7 (2018-07-04)
Mammals produce volatile odours that convey different types of societal information. In Homo sapiens, this is now recognised as body odour, a key chemical component of which is the sulphurous thioalcohol, 3-methyl-3-sulfanylhexan-1-ol (3M3SH). Volatile 3M3SH is produced in the underarm
Hao Lu et al.
Applied biochemistry and biotechnology, 177(1), 105-117 (2015-07-15)
The objective of this study was to understand glucose synthesis of a protein-based artificial photosynthesis system affected by operating conditions, including the concentrations of reactants, reaction temperature, and illumination. Results from non-vesicle-based glyceraldehyde-3-phosphate (GAP) and glucose synthesis showed that the
Ron Simkovitch et al.
Journal of photochemistry and photobiology. B, Biology, 174, 1-9 (2017-07-25)
We used the photoacid 8-hydroxy-1,3,6-pyrenetrisulfonate (HPTS) that converts blue photons to acidic protons in water, with an efficiency of close to 100%, and determined that this treatment conferred changes to colony morphology of the plant pathogen Colletotrichum gloeosporioides. The time
Jeremy R Sanborn et al.
Advanced materials (Deerfield Beach, Fla.), 30(51), e1803355-e1803355 (2018-10-29)
Biological membranes provide a fascinating example of a separation system that is multifunctional, tunable, precise, and efficient. Biomimetic membranes, which mimic the architecture of cellular membranes, have the potential to deliver significant improvements in specificity and permeability. Here, a fully
Yochai Adir et al.
Cancer immunology, immunotherapy : CII, 67(7), 1135-1146 (2018-05-23)
Lung cancer is the leading cause of cancer death worldwide. Survival is largely dependent on the stage of diagnosis: the localized disease has a 5-year survival greater than 55%, whereas, for spread tumors, this rate is only 4%. Therefore, the
Anna Milewska-Hendel et al.
Scientific reports, 9(1), 4724-4724 (2019-03-20)
Uptake of water and nutrients by roots affects the ontogenesis of the whole plant. Nanoparticles, e.g. gold nanoparticles, have a broad range of applications in many fields which leads to the transfer of these materials into the environment. Thus, the
Mark E Polinkovsky et al.
Nature communications, 5, 5737-5737 (2014-12-18)
The temperature-jump technique, in which the sample is rapidly heated by a powerful laser pulse, has been widely used to probe the fast dynamics of folding of proteins and nucleic acids. However, the existing temperature-jump setups tend to involve sophisticated
Esther van Dam et al.
Cell metabolism, 31(4), 710-725 (2020-03-21)
High-sugar diets cause thirst, obesity, and metabolic dysregulation, leading to diseases including type 2 diabetes and shortened lifespan. However, the impact of obesity and water imbalance on health and survival is complex and difficult to disentangle. Here, we show that
Ileana M Pazos et al.
Analytical biochemistry, 483, 21-26 (2015-05-04)
We expand the spectroscopic utility of a well-known infrared and fluorescence probe, p-cyanophenylalanine, by showing that it can also serve as a pH sensor. This new application is based on the notion that the fluorescence quantum yield of this unnatural
Angel Resendez et al.
Organic & biomolecular chemistry, 15(45), 9727-9733 (2017-11-14)
To address carbohydrates that are commonly used in biomedical applications with low binding affinities for boronic acid based detection systems, two chemical modification methods were utilized to increase sensitivity. Modified carbohydrates were analyzed using a two component fluorescent probe based
R L A Timmer et al.
The journal of physical chemistry. A, 114(5), 2091-2101 (2010-01-22)
We studied proton transfer in ice samples containing the photoacid 8-hydroxypyrene-1,3,6-trisulfonic acid and the base sodium formate using femtosecond pump-probe spectroscopy. Pump pulses, centered at a wavelength of 400 nm, electronically excited the photoacid molecules which released their protons. These
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María Nieves Aranda-Sicilia et al.
Biochimica et biophysica acta, 1818(9), 2362-2371 (2012-05-04)
KEA genes encode putative K(+) efflux antiporters that are predominantly found in algae and plants but are rare in metazoa; however, nothing is known about their functions in eukaryotic cells. Plant KEA proteins show homology to bacterial K(+) efflux (Kef)
Patrick Maurer et al.
The Journal of chemical physics, 133(4), 044108-044108 (2010-08-07)
Ultrafast, time-resolved investigations of acid-base neutralization reactions have recently been performed using systems containing the photoacid 8-hydroxypyrene-1,3,6-trisulfonic acid trisodium salt (HPTS) and various Bronsted bases. Two conflicting neutralization mechanisms have been formulated by Mohammed et al. [Science 310, 83 (2005)]
Tomohiro Kaiden et al.
Bioconjugate chemistry, 22(10), 1909-1915 (2011-08-24)
For production of a new type of functional liposome whose destabilization can be triggered by a combination of a temperature signal and acidic pH signal, we prepared liposomes modified with hyperbranched poly(glycidol) derivatives having N-isopropylamide and carboxyl groups. HeLa cells
Mauricio A García et al.
Pharmaceutical research, 37(7), 121-121 (2020-06-10)
MDCK cells are commonly used to assess drug permeability, but the existence of various strains merits a comparative functional study. Since metformin absorption is largely mediated by transporters and paracellular diffusion, we used it to functionally compare MDCK-wt and MDCK-II.
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