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H3784

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Hui Li et al.
Chemistry, an Asian journal, 6(9), 2320-2331 (2011-06-11)
A facile, template-free, and environmentally friendly hydrothermal strategy was explored for the controllable synthesis of α-Fe(2)O(3) nanostructures in HEPES solution (HEPES=2-[4-(2-hydroxyethyl)-1-piperazinyl]ethanesulfonic acid). The effects of experimental parameters including HEPES/FeCl(3) molar ratio, pH value, reaction temperature, and reaction time on the
Hubert Gojzewski et al.
Scanning, 34(3), 159-169 (2011-09-29)
In this study, we deposited isolated magnetosomes from magnetotactic bacteria Magnetospirillum strain AMB-1 onto solid surfaces using spin coating (SC) and drop coating (DC) techniques. Four imaging techniques have been used to visualize the sample structure: scanning and transmission electron
Ines Rosignol et al.
International journal of molecular sciences, 21(5) (2020-03-14)
Mitochondrial damage plays a prominent role in glaucoma. The only way cells can degrade whole mitochondria is via autophagy, in a process called mitophagy. Thus, studying mitophagy in the context of glaucoma is essential to understand the disease. Up to
Anca G Delgado et al.
Microbial cell factories, 11, 128-128 (2012-09-15)
Buffering to achieve pH control is crucial for successful trichloroethene (TCE) anaerobic bioremediation. Bicarbonate (HCO3-) is the natural buffer in groundwater and the buffer of choice in the laboratory and at contaminated sites undergoing biological treatment with organohalide respiring microorganisms.
Ekaterina Isaac et al.
Theriogenology, 161, 273-284 (2020-12-29)
The growth of viable cattle embryos in culture to stages beyond the hatching blastocyst is of interest to developmental biologists wishing to understand developmental events beyond the first lineage decision, as well as for commercial applications, because a lengthening of
R A Romanov et al.
Rossiiskii fiziologicheskii zhurnal imeni I.M. Sechenova, 98(12), 1578-1586 (2013-03-07)
It is widely accepted that ATP secretion in diverse cells involves pannexin 1 (Panx1) that functions as an ATP-permeable channel. We analyzed the permeability of Panx1 channels heterologically expressed in HEK-293 cells to a variety of anions, including ATP. As
Rakesh K Pathak et al.
Analytical chemistry, 84(11), 5117-5123 (2012-05-04)
An in situ prepared Zn(2+) complex of triazole linked imino-thiophenyl conjugate of calix[4]arene, [ZnL], was demonstrated to be highly fluorescent in HEPES buffer solution. [ZnL] has been used as a chemo-sensing ensemble for the recognition of phosphates in general and
Mingyue Li et al.
Structure (London, England : 1993), 27(5), 806-815 (2019-03-19)
The peroxidation of cardiolipins by reactive oxygen species, which is regulated and enhanced by cytochrome c (cyt c), is a critical signaling event in mitochondrial apoptosis. We probe the molecular underpinnings of this mitochondrial death signal through structural and functional
Junbo Li et al.
Dalton transactions (Cambridge, England : 2003), 41(13), 3623-3626 (2012-02-24)
We developed a novel method for Cu(2+) detection based on the ion promoted oxidation reaction. Chemodosimeter L (weak fluorescence) can be oxidized into 3-benzothiazoly-7-N,N-diethylaminocoumarin (strong green fluorescence, coumarin 6) by Cu(2+) with high selectivity and sensitivity in HEPES (10 mM
Sebastian Marwitz et al.
Laboratory investigation; a journal of technical methods and pathology, 94(8), 927-933 (2014-06-17)
Alterations in the DNA methylome are characteristic for numerous diseases and a typical hallmark of cancer. Therefore, DNA methylation is currently under investigation in research labs and has also entered diagnostics. Recently, protocols like the BeadChip technology have become commercially
Madeline Wong et al.
Biochemistry, 52(37), 6548-6558 (2013-09-03)
To date, little work has been conducted on the relationship between solute and buffer molecules and conformational exchange motion in enzymes. This study uses solution NMR to examine the effects of phosphate, sulfate, and acetate in comparison to MES- and
Satyabrata Das et al.
Nature biotechnology, 38(3), 297-302 (2020-02-26)
The scarcity of donor organs may be addressed in the future by using pigs to grow humanized organs with lower potential for immunological rejection after transplantation in humans. Previous studies have demonstrated that interspecies complementation of rodent blastocysts lacking a
Nicole A Renner et al.
Journal of cellular physiology, 228(6), 1284-1294 (2012-11-17)
The foot processes of astrocytes cover over 60% of the surface of brain microvascular endothelial cells, regulating tight junction integrity. Retraction of astrocyte foot processes has been postulated to be a key mechanism in pathology. Therefore, movement of an astrocyte
Jemimah Walker et al.
Journal of biomedical materials research. Part B, Applied biomaterials, 100(4), 1134-1141 (2012-02-15)
Magnesium (Mg) and its alloys have been proposed as degradable replacements to commonly used orthopedic biomaterials such as titanium alloys and stainless steel. However, the corrosion of Mg in a physiological environment remains a difficult characteristic to accurately assess with
Peng Yang
Macromolecular bioscience, 12(8), 1053-1059 (2012-06-19)
When lysozyme is dissolved in a neutral HEPES buffer solution (pH = 7.4) with 0.001-0.050 M TCEP added, a fast phase transition process occurs and the resulting novel fiber-like hierarchical supramolecular assemblies made by primary spherical-particle aggregation can function as a "superglue"
Wen-Yih Jeng et al.
Acta crystallographica. Section D, Biological crystallography, 68(Pt 7), 829-838 (2012-07-04)
NkBgl, a β-glucosidase from Neotermes koshunensis, is a β-retaining glycosyl hydrolase family 1 enzyme that cleaves β-glucosidic linkages in disaccharide or glucose-substituted molecules. β-Glucosidases have been widely used in several applications. For example, mutagenesis of the attacking nucleophile in β-glucosidase
A Akhlaghi et al.
Poultry science, 92(4), 874-881 (2013-03-09)
A hypothesis was tested that the in ovo injection of biological buffers may reinforce the buffering capacity of albumen, thereby withstanding the increase in albumen pH during storage and improving hatchability and chick quality in long-term stored eggs. Hatching eggs
Frauke Pedersen et al.
Respiratory medicine, 107(4), 587-595 (2013-01-15)
Induced sputum is a non-invasive sampling technique for the analysis of airway inflammation in various lung diseases and comprises valuable potential for the identification of biomarkers and therapeutic targets by molecular methods. In the context of biobanking with preservation of
James R Reed et al.
Biochemical pharmacology, 95(2), 126-132 (2015-03-31)
Combustion processes generate particulate matter (PM) that can affect human health. The presence of redox-active metals and aromatic hydrocarbons in the post-combustion regions results in the formation of air-stable, environmentally persistent free radicals (EPFRs) on entrained particles. Exposure to EPFRs
Christian Giaume et al.
Methods in molecular biology (Clifton, N.J.), 814, 283-303 (2011-12-07)
A typical feature of astrocytes is their high level of connexin expression. These membrane proteins constitute the molecular basis of two types of channels: gap junction channels that allow direct cytoplasm-to-cytoplasm communication and hemichannels that provide a pathway for exchanges
Ragnar Thomsen et al.
Drug testing and analysis, 7(7), 565-576 (2014-10-28)
Synthetic cannabimimetic agents are a large group of diverse compounds which act as agonists at cannabinoid receptors. Since 2004, synthetic cannabinoids have been used recreationally, although several of the compounds have been shown to cause severe toxicity in humans. In
Tina Vognsen et al.
Biochemical and biophysical research communications, 420(1), 188-192 (2012-03-15)
The crystal structure of the NTF2-like domain of the Drosophila homolog of Ras GTPase SH3 Binding Protein (G3BP), Rasputin, was determined at 2.7Å resolution. The overall structure is highly similar to nuclear transport factor 2: It is a homodimer comprised
Teruo Shimmen
Journal of plant research, 125(6), 749-754 (2012-03-29)
In the previous paper, I reported that 3,5-dibromo-4-hydroxybenzonitrile (bromoxynil) depolarizes the plasma membrane by inhibiting the electrogenic proton pump and discussed that the inhibition is caused by cytosol acidification due to influx of protonated bromoxynil and following release of proton
James R Reed et al.
Toxicology and applied pharmacology, 289(2), 223-230 (2015-10-02)
Combustion processes generate different types of particulate matter (PM) that can have deleterious effects on the pulmonary and cardiovascular systems. Environmentally persistent free radicals (EPFRs) represent a type of particulate matter that is generated after combustion of environmental wastes in
S Hugel et al.
British journal of pharmacology, 166(8), 2402-2416 (2012-03-29)
Artificial buffers such as HEPES are extensively used to control extracellular pH (pH(e) ) to investigate the effect of H(+) ions on GABA(A) receptor function. In neurones cultured from spinal cord dorsal horn (DH), dorsal root ganglia (DRG) and cerebellar
Jihye Park et al.
The Analyst, 137(19), 4411-4414 (2012-08-07)
We have developed a quickly responsive, and specific fluorescent assay for the detection of Au(III) on the basis of the formation of gold nanoparticles in the presence of HEPES, which cleave the C-I bond of I-BODIPY 1 to yield the
Megan Garvey et al.
Biochemical and biophysical research communications, 409(3), 385-388 (2011-05-18)
The oligomerization of Aβ peptide into amyloid fibrils is a hallmark of Alzheimer's disease. Due to its biological relevance, phosphate is the most commonly used buffer system for studying the formation of Aβ and other amyloid fibrils. Investigation into the
Nilton L Kamiji et al.
Neuroscience research, 72(4), 306-315 (2012-02-14)
It has been postulated that horizontal cells (HCs) send feedback signals onto cones via a proton feedback mechanism, which generates the center-surround receptive field of bipolar cells, and color-opponent signals in many non-mammalian vertebrates. Here we used a strong pH
Yuan Zhou et al.
Toxicology and applied pharmacology, 287(1), 1-8 (2015-05-20)
Microcystin (MC)-LR, a cyclic heptapeptide, is a potent reproductive system toxin. To understand the molecular mechanisms of MC-induced reproductive system cytotoxicity, we evaluated global changes of miRNA and mRNA expression in mouse Sertoli cells following MC-LR treatment. Our results revealed
Nicholas A Courtney et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 34(22), 7645-7656 (2014-05-30)
Metabotropic transmission typically occurs through the spillover activation of extrasynaptic receptors. This study examined the mechanisms underlying somatodendritic dopamine and noradrenaline transmission and found that the extent of spillover and pooling varied dramatically between these two transmitters. In the mouse
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