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Poor Adherent Cell Attachment Troubleshooting
Cells that are cultured under suboptimal growth conditions attach poorly. When the culture surface provided is appropriate, the most common cause of failure of cells to attach to a substrate is environmental stress placed on the cells.
Oxidative Stress in Cell Culture
Read how oxygen, oxygen free radicals, oxidative stress and other cell culture components affect the performance of serum-free, protein-free cell culture systems used for biomanufacturing of monoclonal antibodies
Colorimetric Detection of Western blot using 4-Chloro-1-Naphthol Solution Protocol
Solution contains 0.48 mM 4-Chloro-1-Naphthol, 50 mM Tris-HCl and 0.2 M NaCl in 17% methanol. This is a substrate solution designed for visualizing horseradish peroxidase conjugates in Western Blotting.
Common Cell Culture Problems: Poor Attachment of Adherent Cells
Common Cell Culture Problems: Poor Attachment of Adherent Cells -- Healthy and appropriately nourished cells re-engineer and optimize their attachment matrices. Cells that are cultured under suboptimal growth conditions attach poorly. When the culture surface provided is appropriate, the most...
Riboflavin in Cell Culture
How riboflavin and othercell culture components affect the performance of serum-free, protein-free cell culture systems used for biomanufacturing heterologous proteins including monoclonal antibodies. The page introduces the in vitro chemistry and biochemistry of riboflavin.
Methods Used to Detect Proteins and Nucleic Acids Bound to Membranes
Background and protocols describing the various methods used by molecular biologists to detect samples of protein or nucleic acids bound to membranes.
Selenium in Cell Culture
Understand the role of selenium in cell culture media formulations. The Media Expert library explains how culture media ingredients support cell growth.
Peroxide Forming Solvents
Information on laboratory solvents that can undergo autoxidation under normal storage conditions to form unstable and potentially dangerous peroxide by-products.
Advances of Noble Bimetallic Nanoparticles in Detection of Glucose Level
Dr. David Medina Cruz et. al. investigate advances in noble bimetallic nanoparticles (BMNP) to more precisely measure and monitor glucose levels in the blood. They focus on the use of enzyme-free silver and gold nanoparticles, such as Ag-Au, Ag-Pt, Ag-Pd...
Horseradish Peroxidase (HRP) Enzymes
Discover our peroxidase from horseradish enzymes, products, substrates, and inhibitors for your ELISA, immunoassay, and protein application needs.
Glutathione in Cell Culture
This page providesinsights on how tripeptide, glutathione, and other important cell culture components affect the performance of serum-free, protein-free and animal protein-free cell culture systems used for biomanufacturing heterologous proteins including monoclonal antibodies.
Complete Solutions for IVD Chemiluminescent Immunoassay (CLIA/CLEIA) Development
Accelerate your time to market with fit-for-use products which offer the quality, consistency, and documentation necessary for every step of your IVD development and manufacturing needs.
ICP-MS Analysis of Heavy Metals in Cannabis Sativa
Ensure the safety of cannabis and hemp products by testing for heavy metal contamination using ICP-MS and state specific reference material mixes
Syntheses of Functionalized Alkenes, Arenes, and Cycloalkenes via a Hydroboration-Coupling Sequence
Tandem hydroboration Suzuki Coupling both intermolecular and intramolecular gave diverse alkyl substituted products dppf
Improving the Current USP Method for the Analysis of Lansoprazole Drug Substance Using HPLC Columns Based on Fused-Core® Particle Technology
Compendial methods from the USP (United States Pharmacopeia) are widely used in pharmaceutical drug product and raw materials testing. However, not all methods in the USP use modern technologies. In chromatographic methods, it is not uncommon that older brands of...
Uric Acid in Cell Culture
Uric acid is not a direct additive to any of the classical cell culture media. However, it is present in animal sera. Consequently, uric acid is typically present in complete serum-supplemented classical cell culture systems.
Ferric and Ferrous Iron in Cell Culture
Importance and uses of ferric and ferrous iron in serum-free eukaryotic, including hybridoma and Chinese Hamster Ovary (CHO) cell, cultures
Reference Standards for Analyzing Polyphenol Catechins
Reference Standards for Analyzing Polyphenol Catechins
Titania Nanotubes: Synthesis and Applications
Electronically, it behaves as a wide band gap (3.2 eV) semiconductor and exhibits memristor properties.2 Optically, TiO2 has high opacity with a very high refractive index3 (>2.4), and it exhibits strong absorbance in the UV range.
2D Materials Matter: A Perspective on Biosensing Applications
Professor Ebrahimi and Professor Robinson (Pennsylvania State University, USA) summarize recent advances in the synthesis of these 2D materials, resulting material properties, and related applications in biosensing of neurotransmitters, metabolites, proteins, nucleic acids, bacterial cells, and heavy metals.
Carbohydrate-Catalyzed Enantioselective Alkene Diboration
Enantioselective alkene diboration is a valuable strategy for transforming unsaturated hydrocarbons into useful chiral building blocks.
Lewatit® Ion Exchange Resins – All-around Solutions for Chemical Processes
Chemical processes comprise the steps of processing chemical starting materials, synthesizing the products, isolating the generated materials from the reaction mixture and subsequent purification.
Perfluorosulfonic Acid Membranes for Fuel Cell and Electrolyser Applications
Advances in the electrochemical conversion of water to and from hydrogen and oxygen have principally been achieved through the development of new materials and by understanding the mechanisms of the degradation of proton exchange membrane fuel cells (PEMFC) during operation.
Microbiology Introduction
Introduction to Microbiology Theory. An initial aim of all microbiologists is the reproducible growth of their microbial cultures, no matter whether the microorganisms are of natural origin or have been genetically engineered by man.
Nanomaterials for Advanced Applications
The union of distinct scientific disciplines is revealing the leading edge of Nanotechnology.
Media for Staphylococcus Aureus Detection
Chromogenic media enable the selective detection of S. aureus, which produce bluish-green colonies that are clearly differentiated from other species.
Glucose in Cell Culture
Importance and uses of glucose in serum-free eukaryotic, including hybridoma and Chinese Hamster Ovary (CHO) cell, cultures
Microbiology Theory: Media Preparation
Microorganisms need nutrients, a source of energy and certain environmental conditions in order to grow and reproduce. In the environment, microbes have adapted to the habitats most suitable for their needs, in the laboratory, however, these requirements must be met...
Layer-by-Layer (LbL) Assembly, A "Gentle Yet Flexible" Method Toward Functional Biomaterials
Recently, layer-by-layer (LbL) assembly has emerged as a versatile, gentle and, simple method for immobilization of functional molecules in an easily controllable thin film morphology.1,2 In this short review, we introduce recent advances in functional systems fabricated by using the...
Synthesis and Application of Shape-Controlled Fe3O4 Nanostructure
Professor Yadong Yin (University of California Riverside, USA) examines both direct (thermal decomposition, solvothermal, hydrothermal) and indirect (templated) synthesis methods of magnetite nanocrystals and reviews in detail the landscape of these various synthetic methods for magnetite nanocrystal and their applications...
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