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Carbohydrate determination in honey samples by ion chromatography-mass spectrometry (HPAEC-MS).
Tedesco, et al.
Analytical and Bioanalytical Chemistry, 412, 5217-5227 (2021)
Kristin T Ravndal et al.
Water science and technology : a journal of the International Association on Water Pollution Research, 76(11-12), 2928-2940 (2017-12-07)
A large fraction of the organic substrate in municipal wastewater is particulate. Prior to uptake, particles have to be degraded through potentially a range of intermediates. However, research on intermediate dynamics during particle hydrolysis is limited. In this paper, batch
Daiki Mizushima et al.
Applied microbiology and biotechnology, 103(16), 6581-6592 (2019-07-06)
Paenibacillus sp. 598K produces cycloisomaltooligosaccharides (CIs) in culture from dextran and starch. CIs are cyclic oligosaccharides consisting of seven or more α-(1 → 6)-linked-D-glucose residues. The extracellular enzyme CI glucanotransferase (PsCITase), which is the member of glycoside hydrolase family 66
Andrea Martín-Ortiz et al.
Analytica chimica acta, 1060, 125-132 (2019-03-25)
Carbohydrates are one of the most important ingredients in foods. They are normally present as complex mixtures with different glycosidic linkages, monomeric units and degrees of polymerization. This structural heterogeneity impairs their comprehensive characterization and requires the use of analytical
Synthesis of methyl alpha-isomalto-oligosaccharides specifically deoxygenated at position 2 of the terminal glycopyranosyl unit.
E Petráková et al.
Glycoconjugate journal, 11(1), 17-22 (1994-02-01)
Joana Gangoiti et al.
Scientific reports, 7(1), 9947-9947 (2017-09-01)
The Glycoside hydrolase (GH) family 70 originally was established for glucansucrases of lactic acid bacteria (LAB) converting sucrose into α-glucan polymers. In recent years we have identified 3 subfamilies of GH70 enzymes (designated GtfB, GtfC and GtfD) as 4,6-α-glucanotransferases, cleaving
Milica Fotirić Akšić et al.
Plants (Basel, Switzerland), 8(7) (2019-07-07)
The objective of this study was to determine and compare the sugar profile, distribution in fruits and leaves and sink-source relationship in three strawberry ('Favette', 'Alba' and 'Clery') and three blueberry cultivars ('Bluecrop', 'Duke' and 'Nui') grown in organic (OP)
Manisha Sharma et al.
Bioprocess and biosystems engineering, 42(10), 1681-1693 (2019-07-10)
The genes for dextransucrase and dextranase were cloned from the genomic regions of Leuconostoc mesenteroides MTCC 10508 and Streptococcus mutans MTCC 497, respectively. Heterologous expression of genes was performed in Escherichia coli. The purified enzyme fractions were entrapped in the
Kenji Abe et al.
Applied microbiology and biotechnology, 101(5), 2057-2066 (2016-12-10)
Industrial glucose feedstock prepared by enzymatic digestion of starch typically contains significant amounts of disaccharides such as maltose and isomaltose and trisaccharides such as maltotriose and panose. Maltose and maltosaccharides can be utilized in Escherichia coli fermentation using industrial glucose
Nannan Zhao et al.
Journal of agricultural and food chemistry, 65(43), 9468-9474 (2017-10-06)
We explored the ability of an Aspergillus niger α-glucosidase displayed on P. pastoris to act as a whole-cell biocatalyst (Pp-ANGL-GCW61) system to synthesize isomalto-oligosaccharides (IMOs). IMOs are a mixture that includes isomaltose (IG
Jana Krenkova et al.
Analytica chimica acta, 1095, 226-232 (2019-12-23)
In this work, new multi-cationic aminopyrene-based labeling tags were designed and synthesized for oligosaccharide analysis by capillary electrophoresis-mass spectrometry (CE-MS). The starting compound, 8-aminopyrene-1,3,6-trisulfonic acid trisodium salt, was modified in order to form a sulfonamide derivative having three tertiary amines
Li Jiang et al.
Evidence-based complementary and alternative medicine : eCAM, 2020, 6432942-6432942 (2020-03-19)
Intestinal microbiota plays an important role in human health. The aim of this paper is to determine the impact of the phenolics and carbohydrate in buckwheat honey on human intestinal microbiota. We investigated the phenolics and carbohydrate compositions of eight
T Saito et al.
Bioscience, biotechnology, and biochemistry, 62(7), 1445-1447 (1998-08-28)
A two-dimensional mapping analysis was performed by HPLC for 4 kinds of standard galactosyllactoses (GLs, trisaccharide) which were assumed to be produced from lactose (galactopyranosyl beta 1-->4 glucopyranose) in yogurt during the fermentation of lactic acid bacteria. After the pyridylamination
Hyun-Soo Lee et al.
Journal of agricultural and food chemistry, 50(10), 2812-2817 (2002-05-02)
Maltogenic amylase and alpha-glucanotransferase (alpha-GTase) were employed in an effort to develop an efficient process for the production of isomaltooligosaccharides (IMOs). Bacillus stearothermophilus maltogenic amylase (BSMA) and alpha-GTase from Thermotoga maritima were overexpressed in Escherichia coli using overexpression vectors. An
K Y Wu et al.
Folia microbiologica, 55(6), 582-587 (2011-01-22)
An expression plasmid containing the agdA gene encoding Aspergillus oryzae ZL-1 α-glucosidase was constructed and expressed in Pichia pastoris X-33. The molar mass of the purified protein was estimated by SDS-PAGE. HPLC analysis showed that the purified enzyme has a
Jong-Hyun Jung et al.
Biochemical and biophysical research communications, 483(1), 115-121 (2017-01-04)
Among members of the glycoside hydrolase (GH) family, sucrose isomerase (SIase) and oligo-1,6-glucosidase (O16G) are evolutionarily closely related even though their activities show different specificities. A gene (Avin_08330) encoding a putative SIase (AZOG: Azotobacterglucocosidase) from the nitrogen-fixing bacterium Azotobacter vinelandii
Xiaobei Wang et al.
Biofouling, 32(10), 1223-1233 (2016-10-21)
Dental plaque is a biofilm of water-soluble and water-insoluble polysaccharides, produced primarily by Streptococcus mutans. Dextranase can inhibit biofilm formation. Here, a dextranase gene from the marine microorganism Arthrobacter oxydans KQ11-1 is described, and cloned and expressed using E. coli
Markus C E Belz et al.
International journal of food microbiology, 302, 69-79 (2018-07-19)
The challenge remains for the baking industry to reduce salt levels in yeasted bread as directed by governments, retailers and consumers around the world. The two main problems associated with the reduction of salt are a lack of salty taste
Yoshifumi Oguro et al.
Journal of bioscience and bioengineering, 127(5), 570-574 (2018-11-12)
Koji amazake, prepared from rice koji, is a traditional Japanese sweet beverage. The main source of sweetness is glucose derived from rice starch following digestion by enzymes of Aspergillus oryzae during saccharification. The temperature of this process was empirically determined
Yoshie Maitani et al.
International journal of pharmaceutics, 356(1-2), 69-75 (2008-02-06)
Cationic lipid-based gene delivery systems have shown promise in transfecting cells in vitro and in vivo. However, liposome/DNA complexes tend to form aggregates after preparation. Lyophilization of these systems, therefore, has become of increasing interest. In this study, we investigated
Karan Wangpaiboon et al.
Scientific reports, 8(1), 8340-8340 (2018-05-31)
Alternansucrase catalyses the sequential transfer of glucose residues from sucrose onto another sucrose molecule to form a long chain polymer, known as "alternan". The alternansucrase-encoding gene from Leuconostoc citreum ABK-1 (Lcalt) was successfully cloned and expressed in Escherichia coli. Lcalt
Fan Li et al.
Acta biochimica et biophysica Sinica, 43(4), 324-334 (2011-03-01)
A gene encoding a special thermophilic multifunctional amylase OPMA-N was cloned from Bacillus sp. ZW2531-1. OPMA-N has an additional 124-residue N-terminal domain compared with typical amylases and forms a relatively independent domain with a β-pleated sheet and random coil structure.
Young-Min Kim et al.
Applied microbiology and biotechnology, 85(3), 581-587 (2009-07-15)
Biochemical properties of a putative thermostable dextranase gene from Thermotoga lettingae TMO were determined in a recombinant protein (TLDex) expressed in Escherichia coli and purified to sevenfold apparent homogeneity. The 64-kDa protein displayed maximum activity at pH 4.3, and enzyme
E Shimizu et al.
Bioscience, biotechnology, and biochemistry, 62(1), 117-122 (1998-03-21)
An isomaltotriose-producing endo-dextranase was simply purified from cell-free culture broth of a Fusarium sp. by ethanol fractionation and consecutive column chromatographies using DEAE-Toyopearl and Bio-Gel P-100. The purified enzyme was judged to be homogeneous on PAGE and SDS-PAGE as well
J J Bourgarit et al.
Biochimie, 66(11-12), 711-715 (1984-11-01)
A methodology for the pharmacodynamical assay of mixtures of 2 spasmogenic peptides is described, illustrated and discussed, with reference to a method intended for the chemists (P. Job, 1927). This allows a quick appreciation of the existence of synergism, of
Xiaoying Niu et al.
Journal of agricultural and food chemistry, 56(16), 7271-7278 (2008-08-06)
The feasibility of rapid analysis for oligosaccharides, including isomaltose, isomaltotriose, maltose, and panose, in Chinese rice wine by Fourier transform near-infrared (FT-NIR) spectroscopy together with partial least-squares regression (PLSR) was studied in this work. Forty samples of five brewing years
Matthew Brantley et al.
Journal of the American Society for Mass Spectrometry, 25(10), 1810-1819 (2014-08-07)
Presence of unresolved ion mobility (IM) profiles limits the efficient utilization of IM mass spectrometry (IM-MS) systems for isomer differentiation. Here, we introduce an automated ion mobility deconvolution (AIMD) computer software for streamlined deconvolution of overlapped IM-MS profiles. AIMD is
Xiaolei Li et al.
International journal of biological macromolecules, 114, 235-243 (2018-03-20)
The acidophilic and thermophilic pullulanases have many potential applications in the processes of starch liquefaction and saccharification. In this study, a gene encoding an amylopullulanase from Thermofilum pendens (TPApu) was heterologously expressed in Escherichia coli. Although TPApu possessed the same
R R Russell et al.
The Journal of biological chemistry, 267(7), 4631-4637 (1992-03-05)
An 11-kilobase gene region of Streptococcus mutans has been identified which contains eight contiguous genes involved with the uptake and metabolism of multiple sugars (the msm system). Sequence analysis of this region indicates that several of these genes specify proteins
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