Showing 1-30 of 12368 results for "immunostaining"
John P Frampton et al.
Biotechnology journal, 10(1), 121-125 (2014-07-22)
Conventional immunostaining methods consume large quantities of expensive antibodies and are limited in terms of the number of antigens that can be detected from a single sample. In order to achieve multiplexed immunostaining, we micropatterned antibodies using aqueous two-phase systems...
Anna Stiekema et al.
Cancer cytopathology, 125(3), 197-204 (2017-02-16)
An accurate diagnosis of cancer of Müllerian origin is required before the initiation of treatment. An overlap in clinical presentation and cytological, histological, or imaging studies with other nongynecological tumors does occur. Therefore, immunocytochemistry markers are used to determine tumor...
Jaudah Al-Maghrabi et al.
Saudi journal of gastroenterology : official journal of the Saudi Gastroenterology Association, 25(1), 61-66 (2018-09-19)
The objective of this study was to investigate the relationship between MUC2 immunostaining and clinicopathological characteristics in a subset of colorectal carcinomas (CRCs). A total of 128 CRCs, 50 local nodal metastases, and 42 normal colonic mucosae were retrieved from...
Xu-Tao Chen et al.
Annals of translational medicine, 8(5), 235-235 (2020-04-21)
The positive predictive value (PPV) of urinary decoy cells for diagnosing BK polyomavirus associated-nephropathy (BKPyVAN) is low. This study was designed to increase the PPV of urinary decoy cells for diagnosing BKPyVAN in kidney transplant recipients. A total of 105...
Carla Rossana Scalia et al.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 65(1), 5-20 (2016-11-01)
Antigen masking in routinely processed tissue is a poorly understood process caused by multiple factors. We sought to dissect the effect on antigenicity of each step of processing by using frozen sections as proxies of the whole tissue. An equivalent...
Mahmoud Abd-Elkareem
Animal reproduction science, 180, 100-120 (2017-03-28)
Progesterone receptor alpha (PRA) has a central coordinator role in the ovarian functions in mammals. The aim of this study was to investigate the immunolocalization of PRA in the rabbit ovary during pregnancy and after parturition. The rabbit ovary during...
Aiko Nobusawa et al.
Pathology international, 64(1), 20-27 (2014-01-30)
Diagnosis of the exact grade of oral epithelial dysplasia is difficult, and interobserver variations in grading are common. The aim of this study was to investigate the expression patterns of cytokeratins (CKs) in dysplastic oral epithelia, to identify useful double...
Jong-Shiaw Jin et al.
The Chinese journal of physiology, 59(6), 348-354 (2016-11-07)
Pancreatic ductal adenocarcinoma (PDAC) is an aggressive disease associated with a poor prognosis and high morbidity. Early diagnosis and complete tumor removal are still the principal factors extending life expectancy in PDAC patients. Here, the relationship of neurexophillin 1 (NXPH1)...
Surapathrudu Kanakala et al.
Frontiers in plant science, 7, 1702-1702 (2016-11-30)
Tomato yellow leaf curl virus (TYLCV) is a single-stranded (ssDNA) begomoviruses that causes severe damage to tomato and several other crops worldwide. TYLCV is exclusively transmitted by the sweetpotato whitefly, Bemisia tabaci in a persistent circulative and propagative manner. Previous...
Elenaé Vázquez-Ulloa et al.
Oncotarget, 9(27), 18916-18928 (2018-05-04)
The participation of NOTCH signaling in invasive cervical cancer (ICC) remains controversial since both tumor suppressive and oncogenic properties have been described. Additionally, the role of NUMB, a negative regulator of NOTCH, remains unclear in ICC. We aimed to investigate...
Octavian Bucur et al.
Frontiers in medicine, 5, 322-322 (2018-12-07)
Kidney glomerular diseases, such as the minimal change disease (MCD) and focal segmental glomerulosclerosis (FSGS), and other nephrotic syndromes, are typically diagnosed or confirmed via electron microscopy. Although optical microscopy has been a vital tool to examine clinical specimens for...
Lu Wang et al.
Materials science & engineering. C, Materials for biological applications, 88, 157-165 (2018-04-11)
Hyaluronic acid (HA) is a component of extracellular matrix, which is important for cell functions and tissue integrity. Biosynthesized HA, as well as its derivatives, is widely used in cosmetics industry, biochemical medicine and medical surgery. In this research, we...
Silvia Tapia-González et al.
The Journal of comparative neurology, 528(4), 523-541 (2019-09-13)
Secretagogin (SCGN) is a recently discovered calcium-binding protein belonging to the group of EF-hand calcium-binding proteins. SCGN immunostaining has been described in various regions of the human, rat and mouse brain. In these studies, it has been reported that, in...
Mehmet K Kaya et al.
Clinical & experimental ophthalmology, 43(5), 449-457 (2015-02-03)
The aim of this study was to compare the effects of lapatinib and trastuzumab on vascular endothelial growth factor on experimental corneal neovascularization. A total of 35 Wistar albino rats were randomly divided into five groups, each containing seven animals....
Jennifer Jung et al.
Bio-protocol, 7(17), 27982478-27982478 (2017-10-03)
Autophagy is a recycling pathway, in which intracellular cargoes including protein aggregates and bacteria are engulfed by autophagosomes and subsequently degraded after fusion with lysosomes. Dysregulation of this process is involved in several human diseases such as cancer or neurodegeneration....
R Machaalani et al.
Placenta, 71, 16-23 (2018-11-13)
Cigarette smoking (CS) and preeclampsia (PE), regulate the expression of nicotinic acetylcholine receptor (nAChR) subunits in the placenta, yet no data exist at the histological level. Using immunohistochemistry of formalin fixed and paraffin embedded placental tissue, this study quantified the...
Thays de Andrade Guedes et al.
Journal of environmental science and health. Part. B, Pesticides, food contaminants, and agricultural wastes, 55(8), 687-693 (2020-05-21)
The objective of this research was to investigate the potential damage caused by the residual concentrations of the insecticides Regent® WS 800 and Curbix® SC 200, containing fipronil and ethiprole, respectively as active ingredients, on the liver of Oreochromis niloticus....
Masahiro Satoh et al.
Journal of molecular and cellular cardiology, 128, 77-89 (2019-01-07)
The heart responds to hemodynamic overload through cardiac hypertrophy and activation of the fetal gene program. However, these changes have not been thoroughly examined in individual cardiomyocytes, and the relation between cardiomyocyte size and fetal gene expression remains elusive. We...
B S Pickering et al.
Transboundary and emerging diseases, 65(1), 77-84 (2017-03-28)
Ebolaviruses (family Filoviridae, order Mononegavirales) cause often fatal, haemorrhagic fever in primates including humans. Pigs have been identified as a species susceptible to Reston ebolavirus (RESTV) infection, with indicated transmission to humans in the Philippines; however, their role during Ebola...
Yusha Li et al.
Neurophotonics, 7(1), 015003-015003 (2020-02-06)
Three-dimensional visualization of the innervation in skeletal muscles is helpful for understanding the morphological structure and function. iDISCO, a whole-mount immunolabeling and clearing technique, provides a valuable tool for volume imaging of intramuscular nerve fibers but suffers from the nonspecific...
P Asiabi et al.
Biology of reproduction, 102(1), 145-155 (2019-09-11)
The association between theca cells (TCs) and granulosa cells is pivotal to steroid biosynthesis in the ovary. During the late secondary follicle stage, TCs form a layer around granulosa cells, after which their steroidogenic function falls under the control of...
Takayoshi Ishii et al.
The New phytologist, 222(3), 1652-1661 (2019-03-09)
Visualising the spatio-temporal organisation of the genome will improve our understanding of how chromatin structure and function are intertwined. We developed a tool to visualise defined genomic sequences in fixed nuclei and chromosomes based on a two-part guide RNA with...
L Zhang et al.
Cellular and molecular biology (Noisy-le-Grand, France), 63(4), 16-22 (2017-05-10)
Sphingosine-1-phosphate (S1P) is a sphingolipid in plasma that plays a critical role in cardiovascular and immune systems. Endothelial surface glycocalyx (ESG) decorating the inner wall of blood vessels is a regulator of multiple vascular functions. To test the hypothesis that...
Hisayoshi Ohta et al.
Industrial health, 57(5), 570-579 (2019-01-05)
The role of metallothionein (MT)-like cadmium (Cd) binding protein (MTLCdBP) in protecting the testes against Cd toxicity was examined. In the acute Cd exposure treatment, cadmium chloride was intraperitoneally injected at 2 mg Cd/kg to Wistar male rats. In the chronic...
Zi-Jing Wang et al.
World journal of gastroenterology, 19(44), 8099-8107 (2013-12-07)
To investigate H2B monoubiquitination (uH2B) and H3K4 di- and tri-methylation (H3K4-2me, H3K4-3me) levels and their clinical significance in gastric cancer (GC). Immunohistochemistry (IGC) was used to detect the differential levels of uH2B, H3K4-2me and H3K4-3me modifications in GC specimens from...
G Grandi et al.
Journal of fish biology, 90(5), 1797-1822 (2017-02-02)
The morphogenesis of the pituitary gland and the chronological appearance of adenohypophyseal cells were investigated for the first time in the Somalian cave fish Phreatichthys andruzzii by immunocytochemistry. The adult adenohypophysis contained: a rostral pars distalis, with prolactin (PRL) cells...
Robert J Boucek et al.
Otolaryngology--head and neck surgery : official journal of American Academy of Otolaryngology-Head and Neck Surgery, 149(5), 772-776 (2013-09-07)
Propranolol, a beta 1 (ADBR1) and beta 2 (ADBR2) adrenergic receptor blocker, accelerates regression of proliferating infantile hemangiomas (IH-P) while not affecting non-involuting congenital hemangiomas (NICH) and nonproliferating IH (IH-NP). To determine the expression of ADBRs in vascular tumors, immunofluorescent...
Santhi Devasundaram et al.
Journal of leukocyte biology, 102(1), 135-151 (2017-04-22)
The partial effectiveness against pulmonary tuberculosis (PTB), displayed by the existing tuberculosis (TB) vaccine, bacillus Calmette-Guérin (BCG), highlights the need for novel vaccines to replace or improve BCG. In TB immunology, antigen-specific cellular immune response is frequently considered indispensable. Latency-associated...
Catherine B Campbell et al.
Current protocols in cell biology, 62, Unit 19-Unit 19 (2014-03-13)
Cell adhesion, migration, and signaling in physiologically normal and pathological processes depend highly on the extracellular matrix that the cell interacts with. A variety of in vitro models of two-dimensional and three-dimensional extracellular matrices have been developed to study multiple...
Giovanna Boi et al.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 64(1), 18-31 (2015-10-22)
Drying of the tissue section, partial or total, during immunostaining negatively affects both the staining of tissue antigens and the ability to remove previously deposited antibody layers, particularly during sequential rounds of de-staining and re-staining for multiple antigens. The cause...

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