Showing 1-30 of 170 results for "ion-exchange-column"
Column Cleaning for Ion Exchange Chromatography and Chromatofocusing
This page covers detailed information on cleaning procedures and recommended flow for column cleaning.
Column packing and preparation for Ion Exchange Chromatography & Chromatafocusing
This page shows how to perform column packing and preparation for ion exchange chromatography and chromatafocusing when using Tricorn or XK columns available from Cytiva.
Refolding using ion exchange chromatography and analysis of refolding
This page shows how to refold proteins using ion exchange chromatography with prepacked ion exchange chromatography columns from Cytiva and how to analyze the refolding process with Superdex columns from Cytiva.
Practical Considerations for IEX Separation
This page covers detailed aspects of each step in an IEX separation to improve resolution and overall performance.
LPLC Media & Accessories
Browse through our portfolio to choose from a wide selection of products required for LPLC applications such as columns, a variety of adsorbents, ion exchange resins, separation media, and accessories, designed to fit your unique application demands.
Immobilized Metal Chelate Affinity Chromatography (IMAC)
This page shows how to separate proteins and peptides with affinity for metal ions by immobilized metal chelate affinity chromatography using HiTrap Chelating HP, Chelating Sepharose Fast Flow,His MicroSpin Purification Module or HisTrap Kit from Cytiva.
Purification of Membrane Proteins
This page shows how to perform a purification of His-tagged membrane proteins.
Biopolymer Separations
Supelco’s product offering for biopolymer separations includes columns and media categorized by separation mode, as well as by column brand.
Sepharose Fast Flow: Purification with Good Resolution and Easy Scale-Up
This page covers the use of Sepharose Fast Flow for purification of proteins.
Purification or Removal of Proteins and Peptides
This page shows how to purify or remove proteins and peptides with exposed amino acids with Chelating Sepharose High Performance, Chelating Sepharose Fast Flow, Capto Chelating from Cytiva.
Purification of Histidine-Tagged Proteins using IMAC Sepharose® High Performance
Protocol for Purification of Histidine-Tagged Proteins using IMAC Sepharose® High Performance
Ion Exchange Chromatography Resins
Ion exchange (IEX) chromatography separates biomolecules based on difference in charge between the target molecule and chromatography resin. Widely used in bioprocessing, our anion and cation exchange chromatography resins efficiently purify mAbs, recombinant proteins, vaccines, plasma, and other therapeutic molecules.
Large Molecule HPLC
High performance liquid chromatography (HPLC) can be used to separate and identify different large biomolecules such as protein and peptides in a sample. It is based on the pumping of a sample with a solvent (mobile phase) through a column...
Food & Beverage Analysis: Sugars & Saccharides
Simple sugars using hydrophilic interaction chromatography (HILIC): Chromatographic analysis of simple sugars can be challenging because these compounds are highly polar, uncharged, and lack a chromophore. The preferred mode of HPLC separation is HILIC because it provides the advantage of...
Conditioning Purified Membrane Proteins
This page shows how to condition membrane proteins for further analysis with products from Cytiva.
Ion Chromatography Standards
Our selection of reference standards for ion chromatography includes certified reference material (CRMs) solutions of single- and multi- ions and carbohydrates for the accurate analysis.
Ion Chromatography for Sugar Testing in Food and Environmental Analysis
In this article we demonstrate how well ion chromatography is suited as an analytical technique for sugar analysis e.g. in apple juice, milk, and dust samples.
Downstream purification in bioprocessing includes one or more chromatographic separation steps to purify your molecule and remove impurities. Our broad chromatography portfolio includes resins, membranes, columns, and systems that work seamlessly together to simplify your workflow from process development to...
Small Molecule HPLC
Small molecules are ions and compounds of molecular weight typically less than 900 daltons. These compounds can be effectively separated and analyzed by HPLC, UHPLC and LC-MS using mainly silica particles or monolithic stationary phases with a broad range of...
Purification of Histidine-Tagged Recombinant Proteins Using Ni Sepharose® High Performance
Ni Sepharose High Performance consists of highly cross-linked 6% agarose beads (34 µm) to which a chelating group has been immobilized and subsequently charged with Ni2+ ions.
Purification of Histidine-Tagged Recombinant Proteins Using Ni Sepharose 6 Fast Flow
This page shows how to purify histidine-tagged recombinant proteins using Ni Sepharose 6 Fast Flow from Cytiva. Ni Sepharose 6 Fast Flow consists of 90 µm beads of highly cross-linked agarose, to which a chelating ligand has been immobilized and...
Ion Exchange Chromatography Troubleshooting
This page covers practical problems that may lead to a non-ideal IEX separation and their solutions.
Recombinant/Fusion Tag Protein Purification
Affinity purification resins and buffers for purifying His-tagged, GST-tagged, FLAG® tagged, S-tag™, Strep-Tag II, and T7-tagged proteins under native or denaturing conditions using batch or column purification techniques.
Isolation and LC-MS Characterization of Illicit Bath Salts in Urine
The combination of ion-exchange SPE with the HILIC HPLC separation provides a novel approach for the testing of problematic bath salt compounds.
Sepharose XL – for selected proteins that require very high binding capacity to increase productivity, easy scale-up
This page covers how and when to use Sepharose XL media for purification of proteins.
Selection Guide for Polymeric Resins
These Polymeric resins are appropriate for research-scale separations, as well as, purification and isolation applications. Our product line includes adsorbent, ion-exchange, mixed bed, chelating, nuclear grade, hydrophobic interaction (HIC) and gel filtration/size exclusion products.
HPLC Columns & Accessories
Our wide selection of HPLC columns for small and large molecule analysis allows you to enhance the separation process in terms of retention, resolution, selectivity, and analysis time, all while reducing the time for method development.
LC/MS Analysis of Nucleotides on SeQuant ZIC-cHILIC
HILIC separation is an alternative that permits sensitive MS detection and without the use of ion-pair reagents.
Affinity Chromatography Resins
Affinity chromatography is often used during the capture phase to isolate the target molecule and reduce process volume. Our range of affinity resins includes Protein A, anti-A and anti-B, and metal chelate resin, designed for high throughput and cost-effective purification.
HPLC Analysis of Human Growth Hormone (hGH) on Discovery® BIO PolyMA-WAX
HPLC Analysis of Human Growth Hormone (hGH) on Discovery® BIO PolyMA-WAX

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