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A Khalid et al.
Mikrobiologiia, 75(2), 277-283 (2006-06-09)
Various compounds have been identified as precursors/substrates for the synthesis of ethylene (C2H4) in soil. This study was designed to compare the efficiency of four substrates, namely L-methionine (L-MET), 2-keto-4-methylthiobutyric acid (KMBA), 1-aminocyclopropane-1-carboxylic acid (ACC), and calcium carbide (CaC2) for
Gary W Winston et al.
Comparative biochemistry and physiology. Toxicology & pharmacology : CBP, 139(4), 281-288 (2005-02-03)
During embryogenesis in grass shrimp the capacity to scavenge oxyradicals increased as measured by the Total Oxyradical Scavenging Capacity (TOSC) assay. The increase in TOSC during embryogenesis was associated with increasing concentrations of a number of antioxidants, including coenzyme Q
Zuyu Zheng et al.
Nature chemical biology, 9(4), 244-246 (2013-02-05)
We identify an Arabidopsis pyridoxal-phosphate-dependent aminotransferase, VAS1, whose loss-of-function simultaneously increases amounts of the phytohormone auxin and the ethylene precursor 1-aminocyclopropane-1-carboxylate. VAS1 uses the ethylene biosynthetic intermediate methionine as an amino donor and the auxin biosynthetic intermediate indole-3-pyruvic acid as
Brendan J Hilbert et al.
FEBS letters, 588(9), 1743-1748 (2014-03-25)
The oncogenic corepressors C-terminal Binding Protein (CtBP) 1 and 2 harbor regulatory d-isomer specific 2-hydroxyacid dehydrogenase (d2-HDH) domains. 4-Methylthio 2-oxobutyric acid (MTOB) exhibits substrate inhibition and can interfere with CtBP oncogenic activity in cell culture and mice. Crystal structures of
Charlotte Sandersen et al.
Veterinary anaesthesia and analgesia, 38(2), 83-93 (2011-02-10)
To investigate the effect of acepromazine (ACP) on reactive oxygen species (ROS) production by stimulated equine neutrophils. Ex vivo biochemical experiments. Isolated neutrophils from healthy untreated horses. Neutrophils were incubated with ACP at concentrations of 10(-4), 10(-5) or 10(-6) M
Michael W Straza et al.
Cell cycle (Georgetown, Tex.), 9(18), 3740-3750 (2010-10-12)
The CtBP transcriptional corepressors promote cancer cell survival and migration/invasion. CtBP senses cellular metabolism via a regulatory dehydrogenase domain, and is antagonized by p14/p19(ARF) tumor suppressors. The CtBP dehydrogenase substrate 4-methylthio-2-oxobutyric acid (MTOB) can act as a CtBP inhibitor at
F Regoli et al.
Toxicology and applied pharmacology, 156(2), 96-105 (1999-04-13)
We have extended the application of our previously reported total oxidant scavenging capacity (TOSC) assay (Winston et al., Free Radical Biol. Med. 24, 480-493, 1998) to permit facile quantification of the absorbance capacity of antioxidants toward three potent oxidants, i.e.
Manas K Chattopadhyay et al.
Proceedings of the National Academy of Sciences of the United States of America, 102(45), 16158-16163 (2005-11-02)
Methylthioadenosine is formed during the biosynthesis of spermidine and of spermine and is metabolized by methylthioadenosine phosphorylase, an enzyme missing in several tumor cell lines. In Saccharomyces cerevisiae, this enzyme is coded by the MEU1 gene. We have now studied
Sheng Yin et al.
FEMS microbiology letters, 362(5) (2015-03-07)
Methionol is an important volatile sulfur flavor compound, which can be produced via the Ehrlich pathway in Saccharomyces cerevisiae. Aminotransferase and decarboxylase are essential enzymes catalyzing methionol biosynthesis. In this work, two aminotransferase genes ARO8 and ARO9 and one decarboxylase
Ryan N Dilger et al.
The Journal of nutrition, 137(8), 1868-1873 (2007-07-20)
Relative bioefficacy and toxicity of Met precursor compounds were investigated in young chicks. The effectiveness of DL-Met and 2-keto-4-(methylthio)butyric acid (Keto-Met) to serve as L-Met precursors was quantified using Met-deficient diets of differing composition. Efficacy was based on slope-ratio and
Simona Daniele et al.
Frontiers in aging neuroscience, 10, 17-17 (2018-02-15)
The loss of protein homeostasis that has been associated with aging leads to altered levels and conformational instability of proteins, which tend to form toxic aggregates. In particular, brain aging presents characteristic patterns of misfolded oligomers, primarily constituted of β-amyloid
Lei Jin et al.
Communications biology, 3(1), 349-349 (2020-07-06)
Angiogenesis is required for tissue repair; but abnormal angiogenesis or neovascularization (NV) causes diseases in the eye. The avascular status in the cornea is a prerequisite for corneal clarity and thought to be maintained by the equilibrium between proangiogenic and
Sun Ju Kim et al.
Archives of pharmacal research, 33(6), 867-873 (2010-07-08)
We determined the ability of extracts and active components isolated from nine medicinal plants, Poncirus trifoliata, Astragalus membranaceus, Magnolia obovata, Salvia miltiorrhiza, Angelica dahurica, Cornus officinalis, Cnidium officinale, Pueraria lobata and Ostericum koreanum, to neutralize peroxyl radicals using the total
Yiping Wu et al.
Journal of agricultural and food chemistry, 66(24), 6116-6122 (2018-05-29)
The aminotransferase ARO8 was proved to play an efficient role in conversion of l-methionine into methionol via the Ehrlich pathway in Saccharomyces cerevisiae in our previous work. In this work, the reversible transamination activity of ARO8 for conversion of α-keto-γ-(methylthio)
Gérard Quash et al.
Current medicinal chemistry, 16(28), 3686-3700 (2009-09-15)
Methionine, in addition to its role in protein synthesis, participates in 3 important cellular functions: as AdoMet in transmethylation; as decarboxylated-AdoMet in aminopropylation; as homocysteine its demethylated form, in trans-sulphuration. Here we provide evidence from the literature and from our
Incapacitating CtBP to kill cancer.
Ling-Zun Zhao et al.
Cell cycle (Georgetown, Tex.), 9(18), 3645-3646 (2010-10-12)
Kenza Arfi et al.
Journal of biotechnology, 105(3), 245-253 (2003-10-29)
Two Brevibacterium linens strains and the cheese-ripening yeast Geotrichum candidum were compared with regard to their ability to produce volatile sulfur compounds (VSCs) from three different precursors namely L-methionine, 4-methylthio-2-oxobutyric acid (KMBA) and 4-methylthio-2-hydroxybutyric acid (HMBA). All microorganisms were able
M Arshad et al.
Letters in applied microbiology, 39(3), 306-309 (2004-08-04)
2-Keto-4-methylthiobutyric acid (KMBA) is an established intermediate in microbial biosynthesis of ethylene from methionine. This study demonstrates the kinetics and effects of trace elements and electron complexes on substrate (KMBA)-derived C2H4 biosynthesis in soil. We have previously reported KMBA-dependent C2H4
Hong Li et al.
International journal of biological sciences, 16(7), 1107-1120 (2020-03-17)
Traumatic brain injury (TBI) induces an acute inflammatory response in the central nervous system that involves both resident and peripheral immune cells. The ensuing chronic neuroinflammation causes cell death and tissue damage and may contribute to neurodegeneration. The molecular mechanisms
Baiqing Tang et al.
Biochemical pharmacology, 72(7), 806-815 (2006-07-28)
4-Methylthio-2-oxobutanoic acid (MTOB) is the final compound of the methionine salvage pathway that converts the polyamine byproduct methylthioadenosine to adenine and methionine. Here we find that MTOB inhibits growth of several human cell lines in a dose-dependent manner. Growth inhibition
Ahmad L Subhi et al.
The Journal of biological chemistry, 278(50), 49868-49873 (2003-09-25)
The gene encoding methylthioadenosine phosphorylase (MTAP), the initial enzyme in the methionine salvage pathway, is deleted in a variety of human tumors and acts as a tumor suppressor gene in cell culture (Christopher, S. A., Diegelman, P., Porter, C. W.
Lars Behrendt et al.
F1000Research, 2, 44-44 (2014-02-21)
Cyanobacteria in the genus Acaryochloris have largely exchanged Chl a with Chl d, enabling them to harvest near-infrared-radiation (NIR) for oxygenic photosynthesis, a biochemical pathway prone to generate reactive oxygen species (ROS). In this study, ROS production under different light
D R Livingstone et al.
Marine environmental research, 50(1-5), 57-60 (2001-07-20)
The present study investigated the proposed involvement of contaminant-stimulated reactive oxygen species (ROS) production in disease processes in fish. NAD(P)H-dependent ROS production of subcellular fractions was determined by the iron/EDTA-mediated oxidation of 2-keto-4-methiolbutyric acid. Hepatic cytosolic NADPH-dependent and microsomal NAD(P)H-dependent
Z Findrik et al.
Biotechnology and bioengineering, 98(5), 956-967 (2007-05-31)
D-Methionine was converted to L-methionine in a reaction system where four enzymes were used. D-amino acid oxidase (D-AAO) from Arthrobacter protophormiae was used for the complete conversion of D-methionine to 2-oxo-4-methylthiobutyric acid. Catalase was added to prevent 2-oxo-4-methylthiobutyric acid decarboxylation.
Gerard Quash et al.
Bulletin du cancer, 91(4), E61-E79 (2004-11-26)
We have previously shown that the addition of 4-methylthio-2-oxobutanoate (MTOB) to cultures of methionine dependent neoplastic cells which lack endogenous MTOB restores their capacity to grow in the absence of exogenous methionine. Transition state inhibitors of the MTOB transaminase,responsible for
Joachim Schuster et al.
Plant molecular biology, 57(2), 241-254 (2005-04-12)
Plants are capable to de novo synthesize the essential amino acids leucine, isoleucine and valine. Studies in recent years, however, also revealed that plants have the potential to degrade leucine or may be all of the branched-chain amino acids. One
J Grassmann et al.
Arzneimittel-Forschung, 50(2), 135-139 (2000-03-17)
Pathogenesis and symptoms of inflammatory processes are accompanied and/or initiated by the production of reactive oxygen species (ROS). The effects of essential oils on these processes have been studied with the aid of biochemical model reactions simulating these pathological events.
Do Young Kwon et al.
Toxicological research, 26(4), 321-327 (2010-12-01)
The ability of 80% ethanol extracts from five medicinal plants, Aralia continentalis, Paeonia suffruticosa, Magnolia denudata, Anemarrhena asphodeloides, and Schizonepeta tenuifolia, to neutralize hydroxyl radical, peroxyl radical and peroxynitrite was examined using the total oxyradical scavenging capacity (TOSC) assay. Peroxyl
Miguel García-García et al.
Biotechnology progress, 24(1), 187-191 (2007-12-21)
d-Amino acid oxidase (DAAO) was used to study the oxidative deamination of racemic mixtures of d,l-methionine in its soluble and immobilized forms and thus obtain the corresponding alpha-keto acid. The soluble enzyme form was obtained from a Trigonopsis variabilis CBS
Kimberly L Falk et al.
Phytochemistry, 65(8), 1073-1084 (2004-04-28)
Glucosinolates are a group of sulfur-rich thioglucoside natural products common in the Brassicaceae and related plant families. The first phase in the formation of many glucosinolates involves the chain extension of the amino acid methionine. Additional methylene groups are inserted
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