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PCR assay guide navigates you through primer validation and other assay optimization factors to ensure high sensitivity and specificity for optimum DNA/ RNA quantification.

PCR/qPCR Data Analysis

After a traditional PCR has been completed, the PCR/qPCR data analysis is conducted by resolution through an agarose gel or, more recently, through a capillary.

KiCqStart™ Probe qPCR ReadyMix™

KiCqStart™ Probe qPCR ReadyMix™ is an advanced qPCR reagent system for both fast and conventional PCR cycling protocols or instruments.

Polymerase Chain Reaction

The polymerase chain reaction is one of the most widely used techniques in molecular biology. The PCR process consists of three main steps, Denaturation, Annealing & Extension

Quantitative PCR Basics

Real-time polymerase chain reaction allows researchers to estimate the quantity of starting material in a sample. It has a much wider dynamic range of analysis than conventional PCR

Probe-based qPCR

Probe-based QPCR offers high sensitivity and specificity with fluorescent-labeled probes and various dye options for multiplex amplification.

KiCqStart™ One-Step Probe RT-qPCR

KiCqStart One-Step Probe RT-qPCR ReadyMix streamlines RNA template RT-qPCR with high sensitivity.

SYBR® Green Based Quantitative PCR

SYBR® Green I, a commonly used fluorescent DNA binding dye, binds all double-stranded DNA and detection is monitored by measuring the increase in fluorescence throughout the cycle. Explore our LuminoCt® and KiCqStart® products for Fast qPCR or JumpStart™ reagents for

KiCqStart™ Universal SYBR^® Green qPCR Protocol

Quantitative PCR protocol using SYBR Green reagents. Procedure supports most qPCR instruments.

RT-qPCR – Quantitative Reverse Transcription PCR

RT-qPCR detects specific targets with applications in gene expression and pathogen detection.

Quantitative RT-PCR

RT-qPCR products combine the effective of Reverse Transcriptase with hot-start taq-directed antibody in convenient ReadyMixes for probe-based or SYBR® Green based applications.

Primer Optimization Using Temperature Gradient

Gradient PCR optimizes assay conditions by testing fixed primer concentrations across various annealing temperatures.

qPCR Efficiency Determination Protocol

Optimization of qPCR conditions is important for the development of a robust assay. The two main approaches are optimization of primer concentration and/or annealing temperatures.

qPCR Gene Expression Protocol Using SYBR Green

SYBR Green I dye in qPCR measures target quantity, adaptable to specific needs with a standard protocol.

qPCR Reference Gene Selection Protocol

Analysis of gene expression data requires a stable reference or loading control. This reference is usually one or more reference genes.

How qPCR Works

Watch these videos to learn how real time or quantitative PCR (qPCR) works and the benefits of both the SYBR Green-based and probe-based methods of qPCR assay.

SPUD Assay for Detection of Assay Inhibitors Protocol

SPUD assay identifies inhibitors in RNA or DNA samples, useful for analyzing numerous or low-copy targets.

SYBR® Green I Dye Quantitative PCR Protocol

A protocol that can be used as a basic template for qPCR incorporating SYBR Green I DNA binding dye that is amenable to modification and applicable for use as validation for a set of primers.

Reverse Transcription

Challenges in gene expression analysis include mRNA stability, temporal transcription patterns, and mRNA-protein correlation, impacting accuracy.

Universal SYBR Green qPCR Protocol

Our SYBR Green qPCR Protocol is a method designed to detect accurate quantification of gene expression and RT-PCR reactions

mRNA Synthesis for the Development of Vaccines and Therapeutics

Understand how mRNA vaccines induce immunity. and read how synthetic mRNA is prepared for vaccine immunogens and other biopharmaceuticals. Find reagents for synthesis of mRNA.

PCR Master Mix

PCR master mix simplifies PCR/RT-PCR with components like DNA polymerase, dNTPs, MgCl2, and buffer, available commercially or DIY.

Viral RNA Extraction Buffer for SARS-CoV-2 in Saline and Saliva

Viral RNA Extraction Buffer (VRE100) and related reagents for qRT-PCR and additional viral RNA analyses.

mRNA Synthesis

Tools for plasmid preparation, in vitro transcription, mRNA purification, and LNP formulation in mRNA-based vaccine and therapeutic development.

Ago RIP to Isolate microRNA and their Targets Using Imprint® RNA Immunoprecipitation Kit

Sigma’s Imprint RNA Immunoprecipitation Kit was used to copurify human argonaute 2 (Ago2)-associated RNAs from HeLa cells. MicroRNAs reverse transcribed and quantitating using Mysticq reagents.

Sample Prep & Gel Electrophoresis Troubleshooting

Identify causes and remedies for SDS-PAGE sample preparation challenges and optimize electrophoresis conditions.