Showing 1-30 of 49 results for "

l3022

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Eric P Caragata et al.
Applied and environmental microbiology, 86(11) (2020-03-30)
Given the continued high prevalence of mosquito-transmitted diseases, there is a clear need to develop novel disease and vector control strategies. Biopesticides of microbial origin represent a promising source of new approaches to target disease-transmitting mosquito populations. Here, we describe...
Berit Mueller et al.
Journal of biomaterials applications, 31(8), 1123-1134 (2017-01-26)
An engineered synthetic scaffold for bone regeneration should provide temporary structural support and a medium for controlled and localised release of bioavailable medical drugs. In this work, a method is proposed to incorporate biologically active agents without impairing agent activity...
Mark Soave et al.
Cell chemical biology, 27(10), 1250-1261 (2020-07-02)
Camelid single-domain antibody fragments (nanobodies) offer the specificity of an antibody in a single 15-kDa immunoglobulin domain. Their small size allows for easy genetic manipulation of the nanobody sequence to incorporate protein tags, facilitating their use as biochemical probes. The...
Tanya A Camacho-Villegas et al.
Marine drugs, 16(4) (2018-04-05)
Variable new antigen receptor domain (vNAR) antibodies are novel, naturally occurring antibodies that can be isolated from naïve, immune or synthetic shark libraries. These molecules are very interesting to the biotechnology and pharmaceutical industries because of their unique characteristics related...
João P Fonseca et al.
Bio-protocol, 10(5), e3547-e3547 (2021-03-05)
The ability to rapidly assemble and prototype cellular circuits is vital for biological research and its applications in biotechnology and medicine. The Mammalian ToolKit (MTK) is a Golden Gate-based cloning toolkit for fast, reproducible and versatile assembly of large DNA...
Rosa Ripa et al.
ACS omega, 5(5), 2295-2302 (2020-02-18)
Bacterial biofilms are responsible for persistent infections and biofouling, raising serious concerns in both medical and industrial processes. These motivations underpin the need to develop methodologies to study the complex biological structures of biofilms and prevent their formation on medical...
Bor Kavčič et al.
Nature communications, 11(1), 4013-4013 (2020-08-13)
Antibiotics that interfere with translation, when combined, interact in diverse and difficult-to-predict ways. Here, we explain these interactions by "translation bottlenecks": points in the translation cycle where antibiotics block ribosomal progression. To elucidate the underlying mechanisms of drug interactions between...
Berenice Talamantes-Becerra et al.
BMC microbiology, 20(1), 114-114 (2020-05-15)
This study demonstrates the use of reduced-representation genotyping to provide preliminary identifications for thermophilic bacterial isolates. The approach combines restriction enzyme digestion and PCR with next-generation sequencing to provide thousands of short-read sequences from across the bacterial genomes. Isolates were...
Luying Cui et al.
Developmental and comparative immunology, 105, 103568-103568 (2019-12-11)
Progesterone suppresses the innate immune function of bovine endometrium, making the uterus susceptible to bacterial infection. The bovine endometrial epithelial cells (BEEC) are the first line of defense against bacteria, such as Escherichia coli (E.coli) that causes inflammation of endometrium...
Sun Jin Kim et al.
Biotechnology and bioengineering, 116(7), 1575-1583 (2019-04-02)
Expressing, isolating, and characterizing recombinant proteins is crucial to many disciplines within the biological sciences. Different molecular tagging technologies have been developed to enable each individual step of protein production, from expression through purification and characterization. Monitoring the entire production...
Guang Lu et al.
Autophagy, 15(11), 1917-1934 (2019-03-23)
Macroautophagy/autophagy is a cellular process in which cytosolic contents are degraded by lysosome in response to various stress conditions. Apart from its role in the maintenance of cellular homeostasis, autophagy also involves in regulation of cell cycle progression under nutrient-deprivation...
Luying Cui et al.
Animal reproduction science, 215, 106333-106333 (2020-03-29)
Glucocorticoids have been widely used as anti-inflammatory therapies. The mechanisms of cortisol action in goat does with endometritis, however, have not been reported. The aim of this study was to investigate the mechanism of cortisol in modulation of effects of...
Amirhossein Mafi et al.
PloS one, 16(2), e0246814-e0246814 (2021-02-26)
During formation of the Hedgehog (Hh) signaling proteins, cooperative activities of the Hedgehog INTein (Hint) fold and Sterol Recognition Region (SRR) couple autoproteolysis to cholesterol ligation. The cholesteroylated Hh morphogens play essential roles in embryogenesis, tissue regeneration, and tumorigenesis. Despite...
M Grillo-Puertas et al.
Virulence, 6(6), 608-617 (2015-06-18)
K-12 Escherichia coli cells grown in static media containing a critical phosphate (Pi) concentration ≥25 mM maintained a high polyphosphate (polyP) level in stationary phase, impairing biofilm formation, a phenomenon that is triggered by polyP degradation. Pi concentration in human urine...
Jessica Condi Mainardi et al.
Bioprocess and biosystems engineering, 42(7), 1215-1224 (2019-04-07)
In this work, we present a biocompatible one-pot processing route for ceramic/hydrogel nanocomposites in which we embed live bacteria. In our approach, we fabricate a highly stable alginate hydrogel with minimal shrinkage, highly increased structural and mechanical stability, as well...
Vanda Lux et al.
Structure (London, England : 1993), 28(12), 1288-1299 (2020-09-19)
Dimerization of many eukaryotic transcription regulatory factors is critical for their function. Regulatory role of an epigenetic reader lens epithelium-derived growth factor/p75 (LEDGF/p75) requires at least two copies of this protein to overcome the nucleosome-induced barrier to transcription elongation. Moreover...
Atlas, R. M., L.C. Parks
Handbook of Microbiological Media (1993)
Miriam Zago et al.
PloS one, 16(1), e0245903-e0245903 (2021-01-26)
Lactobacillus helveticus carries many properties such as the ability to survive gastrointestinal transit, modulate the host immune response, accumulate biopeptides in milk, and adhere to the epithelial cells that could contribute to improving host health. In this study, the applicability...
Bernard Charroux et al.
STAR protocols, 1(3), 100117-100117 (2020-12-31)
This protocol is designed to prepare adult axenic Drosophila before monitoring their behavior in a two-choice feeding assay, where flies are confronted with an axenic versus a dead or alive bacteria-contaminated feeding solution. Several aspects of the procedure, including raising...
Reuven Afriat et al.
Talanta, 219, 121223-121223 (2020-09-06)
Rapid and sensitive methods for the detection of pathogenic microorganisms are required to monitor contamination in milk and assure public health. A colorimetric antibody-based stack pad system (biosensor) was developed to detect microbiological content of milk based on nitrocellulose membranes....
Helen K Alexander et al.
Proceedings of the National Academy of Sciences of the United States of America, 117(32), 19455-19464 (2020-07-25)
A better understanding of how antibiotic exposure impacts the evolution of resistance in bacterial populations is crucial for designing more sustainable treatment strategies. The conventional approach to this question is to measure the range of concentrations over which resistant strain(s)...
Alfred Fillol-Salom et al.
Molecular cell, 75(5), 1020-1030 (2019-07-28)
Phage-inducible chromosomal islands (PICIs) represent a novel and universal class of mobile genetic elements, which have broad impact on bacterial virulence. In spite of their relevance, how the Gram-negative PICIs hijack the phage machinery for their own specific packaging and...
Luying Cui et al.
BMC veterinary research, 16(1), 9-9 (2020-01-11)
Bacterial infections are common in postpartum dairy cows. Cortisol level has been observed to increase in dairy cows during peripartum period, and is associated with the endometrial innate immunity against pathogens like E.coli. However, the mechanism underlying how cortisol regulates...
Zuzana Zubáčová et al.
PloS one, 8(3), e55417-e55417 (2013-03-22)
All eukaryotic organisms contain mitochondria or organelles that evolved from the same endosymbiotic event like classical mitochondria. Organisms inhabiting low oxygen environments often contain mitochondrial derivates known as hydrogenosomes, mitosomes or neutrally as mitochondrion-like organelles. The detailed investigation has shown...
[Doubling of the mitral valve].
V A Bukharin et al.
Grudnaia khirurgiia (Moscow, Russia), (5)(5), 15-22 (1978-09-01)
Celia Guilini et al.
Analytical chemistry, 87(17), 8858-8866 (2015-08-12)
Swiftness, reliability, and sensitivity of live bacteria detection in drinking water are key issues for human safety. The most widespread used indicator of live bacteria is a caged form of carboxyfluorescein in which 3' and 6' hydroxyl groups are masked...
Víctor González-Alonso et al.
Poultry science, 99(1), 536-545 (2020-05-18)
The objective of the present study was to assess the potential synergistic effect between supercritical carbon dioxide (SC-CO2) and fresh culinary herbs (Coriandrum sativum and Rosmarinus officinalis) on the microbial inactivation of raw chicken meat. The microbiological inactivation was performed...
Justin A Bosch et al.
Current protocols in molecular biology, 130(1), e112-e112 (2019-12-24)
The CRISPR-Cas9 system makes it possible to cause double-strand breaks in specific regions, inducing repair. In the presence of a donor construct, repair can involve insertion or 'knock-in' of an exogenous cassette. One common application of knock-in technology is to...
Zachary L Fowler et al.
Applied and environmental microbiology, 75(18), 5831-5839 (2009-07-28)
Identification of genetic targets able to bring about changes to the metabolite profiles of microorganisms continues to be a challenging task. We have independently developed a cipher of evolutionary design (CiED) to identify genetic perturbations, such as gene deletions and...
Carlos Sanchez-Martin et al.
Antioxidants & redox signaling, 34(7), 505-516 (2020-05-23)
Aims: TNF receptor-associated protein 1 (TRAP1), the mitochondrial paralog of the heat shock protein 90 (Hsp90) family of molecular chaperones, is required for neoplastic growth in several tumor cell models, where it inhibits succinate dehydrogenase (SDH) activity, thus favoring bioenergetic...