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Protein Expression Systems
The development of genetic engineering and cloning has opened many possibilities of expression and isolation of heterologous proteins for research purposes. Considerable advances in technology have enabled expression and isolation of recombinant proteins in large scale.
Transforming E.coli with Engineered Plasmid
Making Competent Cells; Making Agar Plates; Bacterial Transformation; Picking Colonies; Growing Bacteria in Liquid Culture; Freezing Bacteria.
Bacterial Transformation
Bacterial transformation is a process of horizontal gene transfer by which some bacteria take up foreign genetic material (naked DNA) from the environment. Bacteria that can take up free, extracellular genetic material are known as competent cells.