Showing 1-30 of 30 results for "M0398"
L A Smedly et al.
The Journal of clinical investigation, 77(4), 1233-1243 (1986-04-01)
The neutrophil has been implicated as an important mediator of vascular injury, especially after endotoxemia. This study examines neutrophil-mediated injury to human microvascular endothelial cells in vitro. We found that neutrophils stimulated by formyl-methionyl-leucyl-phenylalanine (FMLP), the complement fragment C5a, or...
Takeshi Kawamura et al.
Microbiology and immunology, 46(3), 225-230 (2002-05-15)
Carboxypeptidase R (EC; CPR) is an unstable basic carboxypeptidase found in fresh serum in addition to carboxypeptidase N (CPN) which is a stable enzyme. CPR in fresh serum is generated from its zymogen (proCPR) during coagulation by trypsin-like enzymes...
R M Senior et al.
The Journal of clinical investigation, 69(2), 384-393 (1982-02-01)
As an approach to facilitating the understanding of proteinases associated with monocytes we have studied U-937 monocytelike cells. Elastase activity was identified in U-937 cell extracts and compared to monocyte elastase activity, neutrophil elastase, and the elastase activity from a...
J R Forney et al.
The Journal of parasitology, 82(4), 638-640 (1996-08-01)
The anticryptosporidial potential of the protease inhibitors alpha-1-antitrypsin (AAT), antipain, aprotinin, leupeptin, methoxysuccinyl-ala-ala-pro-valine chloromethylketone (MAAPVCK), soybean trypsin inhibitor (SBTI), and phenylmethylsulfonyl fluoride (PMSF) was evaluated in a bovine fallopian tube epithelial (BFTE) cell culture system. Protease inhibitor concentrations of 5...
Proteolytic cleavage of annexin 1 by human leukocyte elastase
Rescher U, et al.
Biochimica et Biophysica Acta - Molecular Cell Research, 1763(11), 1320-1324 (2006)
E Ostergaard et al.
APMIS : acta pathologica, microbiologica, et immunologica Scandinavica, 100(12), 1073-1080 (1992-12-01)
The mature neutrophils in the circulation contain, besides the different proteases known for a long time, a recently discovered proteolytically inactive elastase homologue (HBP/CAP37/azurocidin). This homologue, which we have named HBP due to its strong affinity to heparin, is a...
Mona Saffarzadeh et al.
PloS one, 7(2), e32366-e32366 (2012-03-06)
Neutrophils play an important role in innate immunity by defending the host organism against invading microorganisms. Antimicrobial activity of neutrophils is mediated by release of antimicrobial peptides, phagocytosis as well as formation of neutrophil extracellular traps (NET). These structures are...
A Jones et al.
The European respiratory journal, 19(6), 1136-1141 (2002-07-11)
Nonantimicrobial effects of antibiotics may contribute to their activity in the treatment of infective airway disease. The aim of this study was to identify antibiotics used for the treatment of infection in cystic fibrosis that may alter the activity of...
E J Bates et al.
Journal of leukocyte biology, 54(6), 590-598 (1993-12-01)
Previously published work has indicated that polyunsaturated fatty acids (PUFA) may enhance neutrophil-mediated damage to host tissues. We have found that endothelial detachment was significantly increased by neutrophils pretreated with docosahexaenoic (22:6, n-3) and arachidonic (20:4, n-6) acids at 10-40...
Prevention of elastase-induced experimental emphysema by oral administration of a synthetic elastase inhibitor.
A Janoff et al.
The American review of respiratory disease, 121(6), 1025-1029 (1980-06-01)
V S Carl et al.
Immunopharmacology, 33(1-3), 325-329 (1996-06-01)
Bradykinin (BK) is a potent inflammatory mediator, which can release other inflammatory mediators by interacting with bradykinin B1 and B2 receptors. The role of kinins in regulating human PMN elastase release was studied. BK induced elastase release 5-fold over basal...
E Tarján et al.
European journal of respiratory diseases, 64(6), 442-448 (1983-08-01)
The effect of a synthetic elastase inhibitor was studied in experimental emphysema. The development of emphysema was totally prevented by this material. In the authors' opinion the administration of synthetic elastase inhibitors can be considered as a new possibility in...
Martynov, V.F., et al.
J. Gen. Chem., 54, 384-384 (1984)
Erico Vinícius de Souza Carmo et al.
PloS one, 5(11), e13815-e13815 (2010-11-18)
Studies on the role of neutrophils in Leishmania infection were mainly performed with L. (L) major, whereas less information is available for L. (L) amazonensis. Previous results from our laboratory showed a large infiltrate of neutrophils in the site of...
J Tumen et al.
Journal of applied physiology (Bethesda, Md. : 1985), 65(2), 835-843 (1988-08-01)
To determine whether the effects of endotoxin on cultured lung endothelium involve proteolytic mechanisms, we incubated bovine pulmonary arterial endothelial cells with endotoxin in medium 199 + 10% fetal bovine serum (FBS) in the presence and absence of several proteinase...
P S Hiemstra et al.
Immunobiology, 182(2), 117-126 (1991-03-01)
The role of elastase and proteinase inhibitors in polymorphonuclear leukocyte(PMN)-mediated injury to human umbilical cord venous endothelial cells (HUVEC) was investigated. Both purified human neutrophil elastase and PMN that were stimulated with serum-treated zymosan (STZ) induced detachment, but not lysis...
D Polacek et al.
The Journal of biological chemistry, 261(5), 2057-2063 (1986-02-15)
In vitro incubation of human plasma low density lipoproteins (LDL) with human blood polymorphonuclear cells (PMN) for 1 h at 37 degrees C resulted in an increased (2-4-fold) release into the medium of an enzymatic activity which co-eluted with LDL...
Elastin governs the mechanical response of medial collateral ligament under shear and transverse tensile loading
Henninger HB, et al.
Acta Biomaterialia, 25, 304-312 (2015)
I Daudi et al.
Journal of leukocyte biology, 50(4), 331-340 (1991-10-01)
Fragmentation of subendothelial matrix-bound fibronectin by proteases released from stimulated leukocytes has been implicated in lung vascular injury. We studied the degradation of fibronectin bound to denatured collagen by inflammatory polymorphonuclear leukocytes (PMNL). Tissue culture wells coated with denatured collagen...
B O Anderson et al.
Surgery, 108(2), 262-267 (1990-08-01)
Neutrophils have been implicated in multiple models of end-organ injury. The purposes of this study were to determine whether (1) a sublethal septic insult promotes lung neutrophil accumulation, (2) this pulmonary neutrophil accumulation is reversible, (3) these accumulated neutrophils can...
E Tarján et al.
Schweizerische medizinische Wochenschrift, 114(25), 918-920 (1984-06-23)
The inhibitory effect of synthetic elastase inhibitor was studied in experimental emphysema. The development of emphysema was totally prevented by this substance. According to the authors' opinion the administration of synthetic elastase inhibitors can be considered as a new possibility...
J V Edwards et al.
Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society, 7(2), 106-118 (1999-05-07)
A cotton-bound serine protease inhibitor of elastase (fiber-inhibitor) has been formulated for in vitro evaluation in chronic wound fluid. As a model to understand the properties of the inhibitor in wound dressings, the kinetic profile and in vitro release of...
A Molla et al.
Journal of virology, 67(8), 4688-4695 (1993-08-01)
A polyprotein cleavage assay has been developed to assay the proteolytic activities in vitro of the 2A proteinases encoded by poliovirus and human rhinovirus 14, which are representative members of the Enterovirus and Rhinovirus genera of picornaviruses, respectively. The elastase-specific...
D S Fletcher et al.
The American review of respiratory disease, 141(3), 672-677 (1990-03-01)
A pharmacokinetic model is described for testing of polymorphonuclear leukocyte (PMN) elastase inhibitors administered by intratracheal or aerosol dosing of hamsters. Acute lung injury, measured as hemorrhage occurring within hours after intratracheal instillation of human PMN elastase, correlated directly with...
Peptide conjugation to an in vitro selected DNA ligand improves enzyme inhibition
Lin Y, et al.
Proceedings of the National Academy of Sciences of the USA, 92(24), 11044-11048 (1995)
A Z Wei et al.
FEBS letters, 234(2), 367-373 (1988-07-18)
The stoichiometric complex formed between human leukocyte elastase and a synthetic MeO-Suc-Ala-Ala-Pro-Val chloromethyl ketone inhibitor was co-crystallized and its X-ray structure determined, using Patterson search methods. Its structure has been crystallographically refined to a final R value of 0.145 (8.0...
C Edelstein et al.
The Journal of biological chemistry, 272(17), 11079-11087 (1997-04-25)
Incubation of polymorphonuclear cells (PMN), isolated from human peripheral blood, with either lipoprotein(a) (Lp(a)) or free apolipoprotein(a) (apo(a)), derived from the parent Lp(a), caused in both cases a multisite fragmentation of apo(a) inhibited by methoxysuccinyl-Ala-Ala-Pro-Val-CH2Cl, a specific elastase inhibitor. The...
T Herren et al.
Blood, 97(4), 1070-1078 (2001-02-13)
Plasminogen plays an integral role in the inflammatory response, and this participation is likely to depend on its interaction with cell surfaces. It has previously been reported that isolation of human neutrophils from blood leads to a spontaneous increase in...
D S Fletcher et al.
The Journal of pharmacology and experimental therapeutics, 274(1), 548-554 (1995-07-01)
The typical reverse passive Arthus reaction (RPA) was attained in rats by the instillation of a rabbit antiovalbumin serum into the lungs and intravenous injection of ovalbumin. Instillation of antiserum alone caused accumulation of polymorphonuclear leukocytes (PMN) and increased vascular...

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