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J L W Rademaker et al.
Phytopathology, 95(9), 1098-1111 (2008-10-24)
ABSTRACT A comprehensive classification framework was developed that refines the current Xanthomonas classification scheme and provides a detailed assessment of Xanthomonas diversity at the species, subspecies, pathovar, and subpathovar levels. Polymerase chain reaction (PCR) using primers targeting the conserved repetitive
Maria Elena Dell'Aquila et al.
Reproductive biology and endocrinology : RB&E, 2, 44-44 (2004-06-24)
The aim of this study was to investigate cumulus expansion, nuclear maturation and expression of connexin 43, cyclooxygenase-2 and FSH receptor transcripts in equine cumuli oophori during in vivo and in vitro maturation in the presence of equine FSH (eFSH)
Ingmar H Riedel-Kruse et al.
Science (New York, N.Y.), 317(5846), 1911-1915 (2007-08-19)
The "segmentation clock" is thought to coordinate sequential segmentation of the body axis in vertebrate embryos. This clock comprises a multicellular genetic network of synchronized oscillators, coupled by intercellular Delta-Notch signaling. How this synchrony is established and how its loss
Leen Vandaele et al.
Veterinary research, 42, 14-14 (2011-02-15)
Bluetongue virus serotype 8 (BTV-8), which caused an epidemic in ruminants in central Western Europe in 2006 and 2007, seems to differ from other bluetongue serotypes in that it can spread transplacentally and has been associated with an increased incidence
Ting Xu et al.
Scientific reports, 9(1), 1717-1717 (2019-02-10)
Studies of how individual semi-flexible biopolymers and their network assemblies change over time reveal dynamical and mechanical properties important to the understanding of their function in tissues and living cells. Automatic tracking of biopolymer networks from fluorescence microscopy time-lapse sequences
Kee Wui Huang et al.
eLife, 8 (2019-08-15)
The dorsal raphe nucleus (DRN) is an important source of neuromodulators and has been implicated in a wide variety of behavioral and neurological disorders. The DRN is subdivided into distinct anatomical subregions comprised of multiple cell types, and its complex
Ming Fang Hsu et al.
Scientific reports, 8(1), 731-731 (2018-01-18)
The purpose of this study was to develop a gellan gum-based multifunctional embolic agent. Calibrated spherical gellan gum and nanoparticle-containing gellan gum microspheres were prepared via water-in oil emulsification method. Self-assembled nanoparticles composed of short-chain hyaluronic acid and polyethylenimine as
Irena Kukavica-Ibrulj et al.
Journal of bacteriology, 190(8), 2804-2813 (2007-12-18)
Pseudomonas aeruginosa chronic lung infections are the major cause of morbidity and mortality in cystic fibrosis (CF) patients. The P. aeruginosa strains PAO1 and PA14 were compared with the Liverpool epidemic strain LESB58 to assess in vivo growth, infection kinetics
Eugene Y Kim et al.
Clinical immunology (Orlando, Fla.), 127(1), 98-106 (2008-02-16)
The pathogenesis of T cell-mediated diseases like rheumatoid arthritis (RA) has typically been explained in the context of the Th1-Th2 paradigm: the initiation/propagation by pro-inflammatory cytokines, and downregulation by Th2 cytokines. However, in our study based on the adjuvant-induced arthritis
Emma M Roberts et al.
The Journal of endocrinology, 202(3), 453-462 (2009-07-07)
The apelinergic system, comprised of apelin and its G protein-coupled receptor (APJ; APLNR as given in MGI Database), is expressed within key regions of the central nervous system associated with arginine vasopressin (AVP) synthesis and release as well as in
Shujie Shi et al.
Bio-protocol, 7(8) (2017-07-04)
Mechanically-gated ion channels play key roles in mechanotransduction, a process that translates physical forces into biological signals. Epithelial and endothelial cells are exposed to laminar shear stress (LSS), a tangential force exerted by flowing fluids against the wall of vessels
Adrien François et al.
Frontiers in cellular neuroscience, 7, 271-271 (2014-01-09)
The neuronal olfactory epithelium undergoes permanent renewal because of environmental aggression. This renewal is partly regulated by factors modulating the level of neuronal apoptosis. Among them, we had previously characterized endothelin as neuroprotective. In this study, we explored the effect
Keke Shao et al.
PloS one, 6(9), e24910-e24910 (2011-09-29)
Aptamers are short RNA or DNA oligonucleotides which can bind with different targets. Typically, they are selected from a large number of random DNA sequence libraries. The main strategy to obtain aptamers is systematic evolution of ligands by exponential enrichment
Anna Duduś et al.
Applied optics, 54(8), 1952-1957 (2015-05-15)
We report on the fabrication and characterization of a single-mode fiber variable optical attenuator (VOA) based on a ferrofluid shutter actuated by a magnetic field created by a low voltage electromagnet. We compare the performance of a VOA using oil-based
W Buzina et al.
Journal of clinical microbiology, 39(7), 2391-2396 (2001-06-28)
In the last 50 years, to our knowledge, only 16 cases of diseases caused by Schizophyllum commune in humans have been reported. Within only 6 months, we found four isolates of this basidiomycetous fungus, obtained from patients suffering from chronic
Carla Danussi et al.
Molecular and cellular biology, 28(12), 4026-4039 (2008-04-16)
Lymphatic-vasculature function critically depends on extracellular matrix (ECM) and on its connections with lymphatic endothelial cells (LECs). However, the composition and the architecture of ECM have not been fully taken into consideration in studying the biology and the pathology of
Julien Reboud et al.
Proceedings of the National Academy of Sciences of the United States of America, 109(38), 15162-15167 (2012-09-06)
Ultrasonics offers the possibility of developing sophisticated fluid manipulation tools in lab-on-a-chip technologies. Here we demonstrate the ability to shape ultrasonic fields by using phononic lattices, patterned on a disposable chip, to carry out the complex sequence of fluidic manipulations
Jing He et al.
Scientific reports, 5, 10019-10019 (2015-05-07)
Odor detection applications are needed by human societies in various circumstances. Rodent offers unique advantages in developing biologic odor detection systems. This report outlines a novel apparatus designed to train maximum 5 mice automatically to detect odors using a new
Molecular haplotyping by linking emulsion PCR: analysis of paraoxonase 1 haplotypes and phenotypes.
Wetmur J G, et al.
Nucleic Acids Research, 33(8), 2615-2619 (2005)
Liqin Wang et al.
Journal of biomedicine & biotechnology, 2012, 161372-161372 (2012-06-08)
Sheep oocytes derived from the ovaries collected from the slaughterhouse are often used for research on in vitro embryo production, animal cloning, transgenesis, embryonic stem cells, and other embryo biotechnology aspects. Improving the in vitro culture efficiency of oocytes can
Gerrit Cornelis Beekhof et al.
eLife, 10 (2021-05-12)
Distinct populations of Purkinje cells (PCs) with unique molecular and connectivity features are at the core of the modular organization of the cerebellum. Previously, we showed that firing activity of PCs differs between ZebrinII-positive and ZebrinII-negative cerebellar modules (Zhou et
Biodegradation of mineral oils-A review
Aluyor EO and Ori-Jesu M
African Journal of Biotechnology, 8(6), 915-920 (2009)
Scott A Wellnitz et al.
Journal of neurophysiology, 103(6), 3378-3388 (2010-04-16)
Touch is initiated by diverse somatosensory afferents that innervate the skin. The ability to manipulate and classify receptor subtypes is prerequisite for elucidating sensory mechanisms. Merkel cell-neurite complexes, which distinguish shapes and textures, are experimentally tractable mammalian touch receptors that
Kee Wui Huang et al.
Frontiers in cellular neuroscience, 14, 65-65 (2020-04-09)
Viral vectors are essential tools for the study of neural circuits, with glycoprotein-deleted rabies viruses being widely used for monosynaptic retrograde tracing to map connectivity between specific cell types in the nervous system. However, the use of rabies virus is
F J Louws et al.
Applied and environmental microbiology, 60(7), 2286-2295 (1994-07-01)
DNA primers corresponding to conserved motifs in bacterial repetitive (REP, ERIC, and BOX) elements and PCR were used to show that REP-, ERIC-, and BOX-like DNA sequences are widely distributed in phytopathogenic Xanthomonas and Pseudomonas strains. REP-, ERIC, and BOX-PCR
John Keane et al.
Genetics, 164(1), 153-162 (2003-05-17)
Mechanical stimulation induces opposite behavioral responses in the adult and dauer pharynx. Tail tap of adults inhibits pharyngeal pumping via a pathway involving the innexin gene unc-7 and components of the glutamatergic pathway encoded by the genes avr-14 and avr-15.
Michele LeRoux et al.
Molecular cell, 79(2), 280-292 (2020-06-14)
Toxin-antitoxin (TA) systems are ubiquitous genetic elements in bacterial genomes, but their functions are controversial. Although they are frequently postulated to regulate cell growth following stress, few null phenotypes for TA systems have been reported. Here, we show that TA
Nicole C Grieder et al.
PloS one, 3(9), e3241-e3241 (2008-09-20)
There is increasing evidence that tissue-specific modifications of basic cellular functions play an important role in development and disease. To identify the functions of COPI coatomer-mediated membrane trafficking in Drosophila development, we were aiming to create loss-of-function mutations in the
Bart E Vos et al.
Soft matter, 13(47), 8886-8893 (2017-10-24)
Fibrous networks are ideal functional materials since they provide mechanical rigidity at low weight. Here, we demonstrate that fibrous networks of the blood clotting protein fibrin undergo a strong and irreversible increase in their mechanical rigidity in response to uniaxial
Matthew D Dean et al.
Evolution; international journal of organic evolution, 63(1), 20-28 (2008-08-30)
Barriers to gene flow can arise at any stage in the reproductive sequence. Most studies of reproductive isolation focus on premating or postzygotic phenotypes, leaving the importance of differences in fertilization rate overlooked. Two closely related species of house mice
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