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J L Maillard et al.
European journal of cell biology, 52(2), 213-218 (1990-08-01)
A novel beta-lactam derivative, N-(2-chloromethylphenyl) 3,3-difluoroazetidin-2-one, which behaves as a time-dependent inactivator of leukocyte elastase, has been tested in biological models designed to detect its potential therapeutic value in the treatment of emphysema. Its effect on two types of leukocyte...
J V Edwards et al.
Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society, 7(2), 106-118 (1999-05-07)
A cotton-bound serine protease inhibitor of elastase (fiber-inhibitor) has been formulated for in vitro evaluation in chronic wound fluid. As a model to understand the properties of the inhibitor in wound dressings, the kinetic profile and in vitro release of...
Y Zhao et al.
Mucosal immunology, 8(4), 896-905 (2014-12-11)
Neutrophil elastase (NE) and cathepsin G (CG) contribute to intracellular microbial killing but, if left unchecked and released extracellularly, promote tissue damage. Conversely, mechanisms that constrain neutrophil serine protease activity protect against tissue damage but may have the untoward effect...
Kristopher R Genschmer et al.
Cell, 176(1-2), 113-126 (2019-01-12)
Here, we describe a novel pathogenic entity, the activated PMN (polymorphonuclear leukocyte, i.e., neutrophil)-derived exosome. These CD63+/CD66b+ nanovesicles acquire surface-bound neutrophil elastase (NE) during PMN degranulation, NE being oriented in a configuration resistant to α1-antitrypsin (α1AT). These exosomes bind and...
Rendong Fang et al.
Mucosal immunology, 12(5), 1092-1103 (2019-07-07)
It is widely accepted that inflammasomes protect the host from microbial pathogens by inducing inflammatory responses through caspase-1 activation. Here, we show that the inflammasome components ASC and NLRP3 are required for resistance to pneumococcal pneumonia, whereas caspase-1 and caspase-11...
Anna G Drannik et al.
PloS one, 7(4), e35866-e35866 (2012-05-01)
Upon viral recognition, innate and adaptive antiviral immune responses are initiated by genital epithelial cells (ECs) to eradicate or contain viral infection. Such responses, however, are often accompanied by inflammation that contributes to acquisition and progression of sexually transmitted infections...
Octavio Roldán-Padrón et al.
Molecules (Basel, Switzerland), 24(8) (2019-04-25)
Snakebite envenoming is a serious medical problem in different areas of the world. In Latin America, the major prevalence is due to snakes of the family Viperidae, where rattlesnakes (Crotalus) are included. They produce hemotoxic venom which causes bleeding, tissue...
W G Junger et al.
Biological chemistry Hoppe-Seyler, 373(8), 691-698 (1992-08-01)
Sheep are often used to study tissue damage following shock after traumatic injury and in the course of other diseases. The processes involved are thought to be caused at least in part by elastase released from polymorphonuclear leukocytes (PMNs). Since...
Ting Yang et al.
Lab on a chip, 20(15), 2709-2716 (2020-06-24)
Paper-based diagnostic devices have been widely applied to assess the presence and status of a variety of clinical diseases by analyzing samples such as urine or blood. Due to their low cost, user-friendliness, and convenience, they have been used as...
Jing Zhang et al.
Scientific reports, 9(1), 12003-12003 (2019-08-21)
Multiple sclerosis (MS) is a potentially disabling disease of the central nervous system. Approximately half of the patients with MS experience severe pain; however, currently available therapeutics provide only insufficient relief. The mechanisms underlying the generation of neuropathic pain in...
Hanh H Nguyen et al.
Proceedings of the National Academy of Sciences of the United States of America, 105(50), 19750-19755 (2008-12-10)
Indole-3-carbinol (I3C), a naturally occurring component of Brassica vegetables, such as broccoli, cabbage, and Brussels sprouts, induces a G(1) cell-cycle arrest of human breast cancer cells, although the direct cellular targets that mediate this process are unknown. Treatment of highly...
Henry R Maun et al.
Cell, 179(2), 417-431 (2019-10-05)
Severe asthma patients with low type 2 inflammation derive less clinical benefit from therapies targeting type 2 cytokines and represent an unmet need. We show that mast cell tryptase is elevated in severe asthma patients independent of type 2 biomarker...
Md Meraj Ansari et al.
Chemico-biological interactions, 238, 55-65 (2015-06-14)
The precise cause of autoimmune diseases such as rheumatoid arthritis remains uncertain. Collagen induced arthritis (CIA) in animals is the most commonly used model of human rheumatoid arthritis (RA). Exposure of humans and animals to toxic metals is widespread. Cadmium...
Alum triggers infiltration of human neutrophils ex vivo and causes lysosomal destabilization and mitochondrial membrane potential-dependent NET-formation.
Reithofer, et al.
Faseb Journal, 34, 14024-14041 (2021)
Mona Saffarzadeh et al.
PloS one, 7(2), e32366-e32366 (2012-03-06)
Neutrophils play an important role in innate immunity by defending the host organism against invading microorganisms. Antimicrobial activity of neutrophils is mediated by release of antimicrobial peptides, phagocytosis as well as formation of neutrophil extracellular traps (NET). These structures are...
Ulrich Specks et al.
Journal of biochemistry, 141(1), 101-112 (2006-12-13)
Proteinase 3 (PR3) is a neutral serine protease stored in neutrophil granules. It has substantial sequence homology with elastase, cathepsin G and azurocidin. PR3 is the target antigen for autoantibodies (ANCA) in Wegener's granulomatosis, a necrotizing vasculitis syndrome. ANCA have...
S C Wright et al.
Cancer research, 58(23), 5570-5576 (1998-12-16)
Studies on the mechanism of apoptosis in this laboratory support a model in which signal transduction involving caspase 3 leads to activation of a serine protease called Mr 24,000 apoptotic protease (AP24), which then induces internucleosomal DNA fragmentation in the...
Weronika Kuźmicka et al.
Nutrients, 13(1) (2020-12-31)
Zinc plays an important physiological role in the entire body, especially in the immune system. It is one of the most abundant microelements in our organism and an essential component of enzymes and antibacterial proteins. Zinc levels were reported to...
Y M van der Geld et al.
Clinical and experimental immunology, 129(3), 562-570 (2002-08-29)
Anti-neutrophil cytoplasmic antibodies (ANCA) against proteinase 3 (PR3) are strongly associated with Wegener's granulomatosis (WG) and are thought to be involved in its pathogenesis. Levels of PR3-ANCA do not always correspond to clinical disease activity. To investigate the relationship between...
Mapping the extended substrate binding site of cathepsin G and human leukocyte elastase. Studies with peptide substrates related to the alpha 1-protease inhibitor reactive site.
K Nakajima et al.
The Journal of biological chemistry, 254(10), 4027-4032 (1979-05-25)
Carlos A Puyo et al.
Scientific reports, 8(1), 6377-6377 (2018-04-25)
Bladder instrumentation engages the innate immune system via neutrophil activation, promoting inflammation and pain. Elevated levels of mitochondrial DNA (mtDNA) have been associated with tissue damage and organ dysfunction. We hypothesized that local bladder trauma induced by a Foley catheter...
U Specks et al.
FEBS letters, 390(3), 265-270 (1996-07-29)
We developed a stable expression system for conformationally intact recombinant human PR3 (rPR3) using the human mast cell line HMC-1. Like in U937 cells, the rPR3 is processed from a 34 kDa precursor to the 29 kDa mature form, primarily...
Anna G Drannik et al.
PloS one, 7(12), e52738-e52738 (2013-01-10)
Elafin (E) and its precursor trappin-2 (Tr) are alarm antiproteases with antimicrobial and immunomodulatory activities. Tr and E (Tr/E) have been associated with HIV-1 resistance. We recently showed that Tr/E reduced IL-8 secretion and NF-κB activation in response to a...
Hanife Ataoglu et al.
Clinical oral implants research, 13(5), 470-476 (2002-11-28)
The aim of this study was to determine interleukin-1beta (IL-1beta), tumor necrosis factor-alpha (TNF-alpha) levels and neutrophil elastase (NE) activity in peri-implant crevicular fluid (PICF) of smoker and nonsmoker patients, and to investigate their relationships with clinical parameters. A total...
Cynthia M de Bont et al.
Biochimica et biophysica acta. Molecular cell research, 1865(11 Pt A), 1621-1629 (2018-10-18)
Neutrophils can release their chromatin to form neutrophil extracellular traps (NETs), a process known as NETosis. Although NET formation can be induced by various stimuli, recent evidence suggests that these stimuli do so via different mechanisms. Here, we have analysed...