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Paraffin wax as a vapor barrier for the PCR.
Sparkman DR
Genome Research, 2(2), 180-181 (1992)
Matthew D Dean et al.
Evolution; international journal of organic evolution, 63(1), 20-28 (2008-08-30)
Barriers to gene flow can arise at any stage in the reproductive sequence. Most studies of reproductive isolation focus on premating or postzygotic phenotypes, leaving the importance of differences in fertilization rate overlooked. Two closely related species of house mice...
Sven Hildebrand et al.
Scientific reports, 9(1), 10880-10880 (2019-07-28)
Optical clearing techniques and light sheet microscopy have transformed fluorescent imaging of rodent brains, and have provided a crucial alternative to traditional confocal or bright field techniques for thin sections. However, clearing and labeling human brain tissue through all cortical...
Ralph M Jeuken et al.
Journal of biomedical materials research. Part B, Applied biomaterials, 108(8), 3370-3382 (2020-07-03)
Focal knee resurfacing implants (FKRIs) are intended to treat cartilage defects in middle-aged patients. Most FKRIs are metal-based, which hampers follow-up of the joint using magnetic resonance imaging and potentially leads to damage of the opposing cartilage. The purpose of...
Process Fluids for Abrasive Machining
Tribology of Abrasive Machining Processes, 531-585 (2004)
Irena Kukavica-Ibrulj et al.
Journal of bacteriology, 190(8), 2804-2813 (2007-12-18)
Pseudomonas aeruginosa chronic lung infections are the major cause of morbidity and mortality in cystic fibrosis (CF) patients. The P. aeruginosa strains PAO1 and PA14 were compared with the Liverpool epidemic strain LESB58 to assess in vivo growth, infection kinetics...
Toh Miyazaki et al.
Cell reports, 26(4), 1033-1043 (2019-01-24)
Optogenetics requires implantation of light-delivering optical fibers, as current light-sensitive opsins are activated by visible light, which cannot effectively penetrate biological tissues. Insertion of optical fibers and subsequent photostimulation inherently damages brain tissue, and fiber tethering can restrict animal behavior....
Tadas Povilaitis et al.
Protein engineering, design & selection : PEDS, 29(12), 617-628 (2016-09-28)
Compartmentalized self replication (CSR) is widely used for in vitro evolution of thermostable DNA polymerases able to perform PCR in emulsion. We have modified and adapted CSR technique for isothermal DNA amplification using mezophilic phi29 DNA polymerase and whole genome...
Rémi Ducasse et al.
Scientific reports, 7(1), 11344-11344 (2017-09-14)
Artificial bio-based scaffolds offer broad applications in bioinspired chemistry, nanomedicine, and material science. One current challenge is to understand how the programmed self-assembly of biomolecules at the nanometre level can dictate the emergence of new functional properties at the mesoscopic...
Julian Spies et al.
Nature cell biology, 21(4), 487-497 (2019-02-26)
Failure to complete DNA replication is a stochastic by-product of genome doubling in almost every cell cycle. During mitosis, under-replicated DNA (UR-DNA) is converted into DNA lesions, which are inherited by daughter cells and sequestered in 53BP1 nuclear bodies (53BP1-NBs)....
Allison J Lopatkin et al.
Science (New York, N.Y.), 371(6531) (2021-02-20)
Although metabolism plays an active role in antibiotic lethality, antibiotic resistance is generally associated with drug target modification, enzymatic inactivation, and/or transport rather than metabolic processes. Evolution experiments of Escherichia coli rely on growth-dependent selection, which may provide a limited...
J Kabutomori et al.
Biology open, 9(8) (2020-07-15)
Urea transporters (UTs) facilitate urea diffusion across cell membranes and play an important role in the urinary concentration mechanisms in the kidney. Herein, we injected cRNAs encoding for c-Myc-tagged murine UT-B, UT-A2 or UT-A3 (versus water-injected control) in Lithobates oocytes...
Nils Otto et al.
Current biology : CB, 30(16), 3200-3211 (2020-07-04)
Different types of Drosophila dopaminergic neurons (DANs) reinforce memories of unique valence and provide state-dependent motivational control [1]. Prior studies suggest that the compartment architecture of the mushroom body (MB) is the relevant resolution for distinct DAN functions [2, 3]....
Keke Shao et al.
PloS one, 6(9), e24910-e24910 (2011-09-29)
Aptamers are short RNA or DNA oligonucleotides which can bind with different targets. Typically, they are selected from a large number of random DNA sequence libraries. The main strategy to obtain aptamers is systematic evolution of ligands by exponential enrichment...
Leen Vandaele et al.
Veterinary research, 42, 14-14 (2011-02-15)
Bluetongue virus serotype 8 (BTV-8), which caused an epidemic in ruminants in central Western Europe in 2006 and 2007, seems to differ from other bluetongue serotypes in that it can spread transplacentally and has been associated with an increased incidence...
S Nathakarnkitkool et al.
Electrophoresis, 13(1-2), 18-31 (1992-01-01)
Capillary electrophoretic separations of double-stranded DNA fragments in the size range of 20-2200 base pairs were achieved in less than 20 min with the use of a Tris-borate buffer containing hydroxyethylcellulose. Analyses were carried out in both uncoated and phenylmethyl-coated...
Alexandre B Leitão et al.
eLife, 9 (2020-12-29)
Organisms rely on inducible and constitutive immune defences to combat infection. Constitutive immunity enables a rapid response to infection but may carry a cost for uninfected individuals, leading to the prediction that it will be favoured when infection rates are...
Ryota Sakamoto et al.
Nature communications, 11(1), 3063-3063 (2020-06-17)
Symmetric or asymmetric positioning of intracellular structures including the nucleus and mitotic spindle steers various biological processes such as cell migration, division, and embryogenesis. In typical animal cells, both a sparse actomyosin meshwork in the cytoplasm and a dense actomyosin...
Carla Danussi et al.
Molecular and cellular biology, 28(12), 4026-4039 (2008-04-16)
Lymphatic-vasculature function critically depends on extracellular matrix (ECM) and on its connections with lymphatic endothelial cells (LECs). However, the composition and the architecture of ECM have not been fully taken into consideration in studying the biology and the pathology of...
Eugene Y Kim et al.
Clinical immunology (Orlando, Fla.), 127(1), 98-106 (2008-02-16)
The pathogenesis of T cell-mediated diseases like rheumatoid arthritis (RA) has typically been explained in the context of the Th1-Th2 paradigm: the initiation/propagation by pro-inflammatory cytokines, and downregulation by Th2 cytokines. However, in our study based on the adjuvant-induced arthritis...
Emma M Roberts et al.
The Journal of endocrinology, 202(3), 453-462 (2009-07-07)
The apelinergic system, comprised of apelin and its G protein-coupled receptor (APJ; APLNR as given in MGI Database), is expressed within key regions of the central nervous system associated with arginine vasopressin (AVP) synthesis and release as well as in...
Guy Nir et al.
PLoS genetics, 14(12), e1007872-e1007872 (2018-12-27)
Chromosome organization is crucial for genome function. Here, we present a method for visualizing chromosomal DNA at super-resolution and then integrating Hi-C data to produce three-dimensional models of chromosome organization. Using the super-resolution microscopy methods of OligoSTORM and OligoDNA-PAINT, we...
Nicole C Grieder et al.
PloS one, 3(9), e3241-e3241 (2008-09-20)
There is increasing evidence that tissue-specific modifications of basic cellular functions play an important role in development and disease. To identify the functions of COPI coatomer-mediated membrane trafficking in Drosophila development, we were aiming to create loss-of-function mutations in the...
Chee-Huat Linus Eng et al.
Nature methods, 14(12), 1153-1155 (2017-11-14)
Single-molecule FISH (smFISH) has been the gold standard for quantifying individual transcript abundances. Here, we scale up multiplexed smFISH to the transcriptome level and profile 10,212 different mRNAs from mouse fibroblast and embryonic stem cells. This method, called RNA sequential...
Amanda N Samuels et al.
Microorganisms, 8(5) (2020-05-07)
Colicins are specific and potent toxins produced by Enterobacteriaceae that result in the rapid elimination of sensitive cells. Colicin production is commonly found throughout microbial populations, suggesting its potential importance for bacterial survival in complex microbial environments. Nonetheless, as colicin...
Thomas Litschel et al.
Nature communications, 12(1), 2254-2254 (2021-04-17)
One of the grand challenges of bottom-up synthetic biology is the development of minimal machineries for cell division. The mechanical transformation of large-scale compartments, such as Giant Unilamellar Vesicles (GUVs), requires the geometry-specific coordination of active elements, several orders of...
Andrea R Victor et al.
Fertility and sterility, 111(2), 280-293 (2019-01-30)
To investigate the parameters of mosaicism and the biological mechanisms leading to healthy pregnancies from mosaic embryo transfers. Prospective study. IVF center and associated research laboratory. Fifty-nine patients. Embryos underwent blastocyst-stage preimplantation genetic testing for aneuploidy by next-generation sequencing. Trophectoderm...
Gowoon Son et al.
Alzheimer's research & therapy, 13(1), 4-4 (2021-01-06)
Hyposmia in Alzheimer's disease (AD) is a typical early symptom according to numerous previous clinical studies. Although amyloid-β (Aβ), which is one of the toxic factors upregulated early in AD, has been identified in many studies, even in the peripheral...
Qiong Pan et al.
Analytica chimica acta, 1000, 214-222 (2018-01-01)
Arrays of microwells are widely used to isolate individual cells, facilitate high throughput cytometry assays, and ensure compatibility of those assays with whole-cell imaging. Microwell geometries have recently been utilized for handling and preparation of single-cell lysate, prior to single-cell...
Lei Gao et al.
Cell, 173(1), 248-259 (2018-03-13)
The dynamics of the chromatin regulatory landscape during human early embryogenesis remains unknown. Using DNase I hypersensitive site (DHS) sequencing, we report that the chromatin accessibility landscape is gradually established during human early embryogenesis. Interestingly, the DHSs with OCT4 binding...
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