Showing 1-30 of 40 results for "M6030"
Matthew J Culyba et al.
PLoS genetics, 14(6), e1007405-e1007405 (2018-06-02)
Biochemical pathways are often genetically encoded as simple transcription regulation networks, where one transcription factor regulates the expression of multiple genes in a pathway. The relative timing of each promoter's activation and shut-off within the network can impact physiology. In...
Nicolo' Politi et al.
Journal of biological engineering, 8(1), 5-5 (2014-02-04)
Inducible promoters are widely spread genetic tools for triggering, tuning and optimizing the expression of recombinant genes in engineered biological systems. Most of them are controlled by the addition of a specific exogenous chemical inducer that indirectly regulates the promoter...
Difco Manual, 277-277 (1998)
Miller, J.H.
Experiments in Molecular Genetics, 433-433 (1972)
P Naves et al.
Journal of applied microbiology, 105(2), 585-590 (2008-03-28)
In this study, we have evaluated the impact of methodological approaches in the determination of biofilm formation by four clinical isolates of Escherichia coli in static assays. The assays were performed in microtitre plates with two minimal and two enriched...
Alexander Perniss et al.
Immunity, 52(4), 683-699 (2020-04-16)
Mucociliary clearance through coordinated ciliary beating is a major innate defense removing pathogens from the lower airways, but the pathogen sensing and downstream signaling mechanisms remain unclear. We identified virulence-associated formylated bacterial peptides that potently stimulated ciliary-driven transport in the...
Ilias Lagkouvardos et al.
Nature microbiology, 1(10), 16131-16131 (2016-09-28)
Intestinal bacteria influence mammalian physiology, but many types of bacteria are still uncharacterized. Moreover, reference strains of mouse gut bacteria are not easily available, although mouse models are extensively used in medical research. These are major limitations for the investigation...
Lauren B A Woodruff et al.
Nucleic acids research, 45(3), 1553-1565 (2016-12-23)
Genetic designs can consist of dozens of genes and hundreds of genetic parts. After evaluating a design, it is desirable to implement changes without the cost and burden of starting the construction process from scratch. Here, we report a two-step...
Amar Ghodasara et al.
Nucleic acids research, 45(13), 8116-8127 (2017-06-14)
Balancing protein expression is critical when optimizing genetic systems. Typically, this requires library construction to vary the genetic parts controlling each gene, which can be expensive and time-consuming. Here, we develop sRNAs corresponding to 15nt 'target' sequences that can be...
Xinyi Wan et al.
Nature chemical biology, 15(5), 540-548 (2019-03-27)
Cell-based biosensors have great potential to detect various toxic and pathogenic contaminants in aqueous environments. However, frequently they cannot meet practical requirements due to insufficient sensing performance. To address this issue, we investigated a modular, cascaded signal amplifying methodology. We...
Stephanie K Aoki et al.
Molecular microbiology, 70(2), 323-340 (2008-09-03)
Contact-dependent growth inhibition (CDI) is a phenomenon by which bacterial cell growth is regulated by direct cell-to-cell contact via the CdiA/CdiB two-partner secretion system. Characterization of mutants resistant to CDI allowed us to identify BamA (YaeT) as the outer membrane...
Intestinimonas butyriciproducens gen. nov., sp. nov., a butyrate-producing bacterium from the mouse intestine.
Klaring K
International Journal of Systematic and Evolutionary Microbiology, 63, 4606-4612 (2013)
Amin Espah Borujeni et al.
Nature communications, 11(1), 5001-5001 (2020-10-07)
To perform their computational function, genetic circuits change states through a symphony of genetic parts that turn regulator expression on and off. Debugging is frustrated by an inability to characterize parts in the context of the circuit and identify the...
Clowes, R.C., and Hayes, W., ed.
Experiments in Microbial Genetics, 187-187 (1968)
Stavroula K Hatzios et al.
Biochemistry, 47(21), 5823-5831 (2008-05-06)
Mycobacterium tuberculosis ( Mtb) produces a number of sulfur-containing metabolites that contribute to its pathogenesis and ability to survive in the host. These metabolites are products of the sulfate assimilation pathway. CysQ, a 3'-phosphoadenosine-5'-phosphatase, is considered an important regulator of...
Zhanmin Fan et al.
PloS one, 4(2), e4432-e4432 (2009-02-13)
Escherichia coli is capable of producing hydrogen under anaerobic growth conditions. Formate is converted to hydrogen in the fermenting cell by the formate hydrogenlyase enzyme system. The specific hydrogen yield from glucose was improved by the modification of transcriptional regulators...
Laboratory evolution of Escherichia coli thioredoxin for enhanced catalysis of protein oxidation in the periplasm reveals a phylogenetically conserved substrate specificity determinant.
Masip L
The Journal of Biological Chemistry, 283(2), 840-848 (2008)
Sambrook, J., et al.
Molecular Cloning: A Laboratory Manual, A-A (1989)
Terence S Crofts et al.
Nature chemical biology, 14(6), 556-564 (2018-05-02)
The soil microbiome can produce, resist, or degrade antibiotics and even catabolize them. While resistance genes are widely distributed in the soil, there is a dearth of knowledge concerning antibiotic catabolism. Here we describe a pathway for penicillin catabolism in...
David Chen et al.
Molecular systems biology, 8, 620-620 (2012-10-24)
Natural biological systems have evolved a diverse array of switches to realize their strategies for environmental response and development. Emerging applications of synthetic biology have begun to exploit such switches to achieve increasingly sophisticated designed behaviors. However, not all switch...
Nancy Valentine et al.
Applied and environmental microbiology, 71(1), 58-64 (2005-01-11)
Matrix-assisted laser desorption ionization-time-of-flight mass spectrometry (MALDI-TOF MS) has been used to identify bacteria based upon protein signatures. This research shows that while some different proteins are produced by vegetative bacteria when they are cultured in different growth media, positive...
Amanda N Samuels et al.
Infection and immunity, 87(2) (2018-12-05)
Bacteria have a remarkable ability to survive, persist, and ultimately adapt to environmental challenges. A ubiquitous environmental hazard is DNA damage, and most bacteria have evolved a network of genes to combat genotoxic stress. This network is known as the...
Davis, R.W., et al.
A Manual for Genetic Engineering: Advanced Bacterial Genetics, 204-204 (1980)
Adams, M.H.
Bacteriophages, 446-446 (1959)
Jochem N A Vink et al.
Molecular cell, 77(1), 39-50 (2019-11-19)
CRISPR-Cas systems encode RNA-guided surveillance complexes to find and cleave invading DNA elements. While it is thought that invaders are neutralized minutes after cell entry, the mechanism and kinetics of target search and its impact on CRISPR protection levels have...
Vittorio Bartoli et al.
Nature communications, 11(1), 2095-2095 (2020-05-01)
Synthetic genetic circuits allow us to modify the behavior of living cells. However, changes in environmental conditions and unforeseen interactions with the host cell can cause deviations from a desired function, resulting in the need for time-consuming reassembly to fix...
Aixia Zhang et al.
Molecular medicine reports, 16(2), 1321-1327 (2017-06-20)
Triclosan (TCS) is widely used as broad-spectrum antibacterial agent. However, it may threaten the health of human offspring if the mother is exposed to TCS during pregnancy. The present study aimed to identify potential mechanisms behind the toxic effect of...
Omri M Finkel et al.
Nature, 587(7832), 103-108 (2020-10-02)
Plants grow within a complex web of species that interact with each other and with the plant1-10. These interactions are governed by a wide repertoire of chemical signals, and the resulting chemical landscape of the rhizosphere can strongly affect root...

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