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Amino Acid Solutions - M7145
M5550for.doc
Amino Acid Solutions
B 6766 M 5550 M 7145 R 7131
BME [50X] MEM [50X] MEM [100X] RPMI-1640 [50X]
COMPONENT g/L g/L g/L g/L
Product Information Sheet - A7469
in cell
culture media and is a component of MEM non-
essential amino acids solution (Product No. M7145).
Precautions and Disclaimer
For Laboratory Use Only. Not for drug, household or
other uses
A non-scrubbing protocol for use with adherent cells in chemotaxis assays using MultiScreen-MIC plates
Culture Media: RPMI (Cat. R8758), culture media components MEM non-essential amino acids (NEAA)
(Cat. M7145), HEPES buffer (Cat. H0887) and L-glutamine-penicillin/streptomycin solution (Cat. G1146) were
obtained
H2A.X Phosphorylation Assay Kit Chemiluminescence Detection
Sodium Pyruvate: (Specialty Media, TMS-005-C), 100mM, 100X
5. Non-Essential Amino Acid: (Sigma, M7145), 100X
6. 100X Etoposide: #90533, a 10 mM stock in DMSO. Final concentration will be 100 μM.
Product Information Sheet - I3411
(1mM)
Sigma - Cat. No. S8636
• MEM Non-essential amino acid solution (1%)
Sigma - Cat. No. M7145
• Penicillin-Streptomycin (optional) (1%)
Sigma - Cat. No. P4333
• HIV-1 p24 Antigen ELISA Kit
MultiScreen Caco-2 --Drug Transport Assembly in a 96-well system:
C02 S5).
3
Materials and Equipment
Tissue Culture
Non-essential amino acids (cat.
M7145), HEPES (cat. H0887),
penicillin, streptomycin and
L-glutamine (cat. G1146), EDTA
(cat. E8008
Evaluation of MultiScreen-MIC Plates in Invasion and Angiogenesis Assays
Culture Media: RPMI (Cat. R8758), culture media components MEM non-essential amino
acids (NEAA) (Cat. M7145), HEPES buffer (Cat. H0887) and L-glutamine-
penicillin/streptomycin solution (Cat. G1146) were obtained
Evaluation of MultiScreen-MIC Plates in Chemotaxis Assays
Cat. D5796), RPMI (Cat. R8758), culture media
components MEM non-essential amino acids (NEAA) (Cat. M7145), HEPES buffer (Cat.
H0887) and L-glutamine-penicillin/streptomycin solution (Cat. G1146) were obtained