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m7383

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Lin-Na Zhu et al.
Gene, 532(2), 246-252 (2013-09-24)
Skeletal muscle fiber type composition is one of the important factors influencing muscle growth and meat quality. As a member of the myogenic transcription factors, myogenin (MyoG) is required for embryonic myoblast differentiation, but the expression of MyoG continues in
J C Venter et al.
Science (New York, N.Y.), 291(5507), 1304-1351 (2001-02-22)
A 2.91-billion base pair (bp) consensus sequence of the euchromatic portion of the human genome was generated by the whole-genome shotgun sequencing method. The 14.8-billion bp DNA sequence was generated over 9 months from 27,271,853 high-quality sequence reads (5.11-fold coverage
G Harish et al.
Neurochemical research, 38(1), 186-200 (2012-10-17)
Altered redox dynamics contribute to physiological aging and Parkinson's disease (PD). This is reflected in the substantia nigra (SN) of PD patients as lowered antioxidant levels and elevated oxidative damage. Contrary to this observation, we previously reported that non-SN regions
Purification and properties of Drosophila malate dehydrogenases.
M S McReynolds et al.
Biochimica et biophysica acta, 198(2), 165-175 (1970-02-11)
Yumi Morimoto et al.
Journal of bioscience and bioengineering, 117(2), 147-152 (2013-08-13)
The directed evolution of the thermotolerant NADP(H)-dependent malic enzyme from Thermococcus kodakarensis was conducted to alter the cofactor preference of the enzyme from NADP(H) to NAD(H). The construction and screening of two generations of mutant libraries led to the isolation
Toshio Ota et al.
Nature genetics, 36(1), 40-45 (2004-01-01)
As a base for human transcriptome and functional genomics, we created the "full-length long Japan" (FLJ) collection of sequenced human cDNAs. We determined the entire sequence of 21,243 selected clones and found that 14,490 cDNAs (10,897 clusters) were unique to
Yan He et al.
PloS one, 8(3), e57118-e57118 (2013-03-28)
Maintaining metabolic homeostasis is critical for plant growth and development. Here we report proteome and metabolome changes when the metabolic homeostasis is perturbed due to gene-dosage dependent mutation of Arabidopsis isopropylmalate dehydrogenases (IPMDHs). By integrating complementary quantitative proteomics and metabolomics
Clelia Muccio et al.
The protein journal, 31(8), 667-673 (2012-09-12)
A novel malate dehydrogenase (MDH; EC 3.1.1.1.37), hereafter MDHCs, from Ceratonia siliqua seeds, commonly known as Carob tree, was purified by using ammonium sulphate precipitation, ion exchange chromatography on SteamLine SP and gel-filtration. The molecular mass of the native protein
Chao Wang et al.
Biochimica et biophysica acta, 1834(10), 1969-1975 (2013-07-09)
Rhodotorula taiwanensis RS1 is a high-aluminum (Al)-tolerant yeast that can survive in Al concentrations up to 200mM. The mechanisms for the high Al tolerance of R. taiwanensis RS1 are not well understood. To investigate the molecular mechanisms underlying Al tolerance
X M Men et al.
Animal : an international journal of animal bioscience, 7(9), 1486-1492 (2013-06-15)
The objective of this study is to investigate the age-related changes of and the effects of dietary conjugated linoleic acid (CLA) on muscle-fibre types in commercial pigs. We divided 25 crossbred male pigs into five age groups (7, 30, 60
Qiong Liu et al.
The Prostate, 73(10), 1028-1037 (2013-02-08)
Resistance to chemotherapy represents a significant obstacle in prostate cancer therapeutics. Novel mechanistic understandings in cancer cell chemotherapeutic sensitivity and resistance can optimize treatment and improve patient outcome. Molecular alterations in the metabolic pathways are associated with cancer development; however
Teresa Z Rzezniczak et al.
G3 (Bethesda, Md.), 2(12), 1613-1623 (2013-01-01)
Interactions across biological networks are often quantified under a single set of conditions; however, cellular behaviors are dynamic and interactions can be expected to change in response to molecular context and environment. To determine the consistency of network interactions, we
Eun Young Jung et al.
Annals of nutrition & metabolism, 61(2), 89-94 (2012-08-15)
We observed whether the anti-obesity activity of yeast hydrolysate (YH) was due to the alteration of lipid-regulating enzyme activities. Male ICR mice were divided into four groups: a normal diet group (ND; 4.2% fat), a high-fat diet group (HF; 27.7%
Allan E Strand et al.
PloS one, 7(9), e45138-e45138 (2012-10-11)
A common geographical pattern of genetic variation is the one-dimensional cline. Clines may be maintained by diversifying selection across a geographical gradient but can also reflect historical processes such as allopatry followed by secondary contact. To identify loci that may
Kwang Suk Lim et al.
Journal of controlled release : official journal of the Controlled Release Society, 171(2), 193-200 (2013-07-23)
Protein transduction domains (PTDs), also known as cell-penetrating peptides (CPPs), have been developed as effective systems for delivering bio-active cargos such as proteins, genes and particles. Further improvements on cell-specific targeting, intracellular organelle targeting and intracellular retention are still necessary
S Cyrus Khojasteh et al.
Chemical research in toxicology, 25(11), 2301-2309 (2012-10-31)
Pulegone is the major constituent of pennyroyal oil, a folkloric abortifacient that is associated with hepatotoxicity and, in severe cases, death. Cytochrome P450-mediated oxidation of pulegone generates menthofuran, which is further oxidized to form electrophilic reactive intermediates, menthofuran epoxide and
Jesper V Olsen et al.
Science signaling, 3(104), ra3-ra3 (2010-01-14)
Eukaryotic cells replicate by a complex series of evolutionarily conserved events that are tightly regulated at defined stages of the cell division cycle. Progression through this cycle involves a large number of dedicated protein complexes and signaling pathways, and deregulation
Morgan Kullberg et al.
Molecular biology and evolution, 23(8), 1493-1503 (2006-06-06)
The molecular relationship of placental mammals has attracted great interest in recent years. However, 2 crucial and conflicting hypotheses remain, one with respect to the position of the root of the eutherian tree and the other the relationship between the
Frederik M Meyer et al.
FEMS microbiology letters, 339(1), 17-22 (2012-11-10)
The Gram-positive soil bacterium Bacillus subtilis uses glucose and malate as the preferred carbon sources. In the presence of either glucose or malate, the expression of genes and operons for the utilization of secondary carbon sources is subject to carbon
Thomas R Burkard et al.
BMC systems biology, 5, 17-17 (2011-01-29)
On the basis of large proteomics datasets measured from seven human cell lines we consider their intersection as an approximation of the human central proteome, which is the set of proteins ubiquitously expressed in all human cells. Composition and properties
Mary-Rus Martínez-Cuenca et al.
Tree physiology, 33(3), 320-329 (2013-03-07)
The effects of iron (Fe) deficiency on the low-molecular-weight organic acid (LMWOA) metabolism have been investigated in Carrizo citrange (CC) [Citrus sinensis (L.) Osb. × Poncirus trifoliata (L.) Raf.] roots. Major LMWOAs found in roots, xylem sap and root exudates were citrate
Rosa Amarilis Rodríguez-Frómeta et al.
Applied microbiology and biotechnology, 97(7), 3063-3072 (2012-10-12)
Commercial interest in microbial lipids is increasing due to their potential use as feedstock for biodiesel production. The supply of NADPH generated by malic enzyme (ME; NADP+-dependent; EC 1.1.1.40) has been postulated as being the rate-limiting step for fatty acid
Kevork Hagopian et al.
Life sciences, 93(24), 941-948 (2013-10-22)
The purpose of the study was to establish if enzyme activities from key metabolic pathways and levels of markers of oxidative damage to proteins and lipids differed between distinct liver mitochondrial sub-populations, and which specific sub-populations contributed to these differences.
Xiaoting Ye et al.
Microbial cell factories, 11, 120-120 (2012-09-07)
The integration of biotechnology into chemical manufacturing has been recognized as a key technology to build a sustainable society. However, the practical applications of biocatalytic chemical conversions are often restricted due to their complexities involving the unpredictability of product yield
Heng B Xie et al.
PLoS genetics, 9(6), e1003544-e1003544 (2013-07-03)
Dominant mutations in the alpha-B crystallin (CryAB) gene are responsible for a number of inherited human disorders, including cardiomyopathy, skeletal muscle myopathy, and cataracts. The cellular mechanisms of disease pathology for these disorders are not well understood. Among recent advances
Cindy D Crocker et al.
Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology, 166(2), 141-147 (2013-08-27)
The African cichlid, Pseudocrenilabrus multicolor victoriae is a eurytopic fish that exhibits high levels of developmental plasticity in response to dissolved oxygen availability. In this study, F1 offspring from three sites in the Mpanga River drainage of Western Uganda characterized
P Benoist et al.
Analytical biochemistry, 187(2), 337-344 (1990-06-01)
Nanogram amounts of soluble aminopeptidases, dehydrogenases, and esterases were detected by nondenaturing ultralow gelling point agarose-polyacrylamide gel electrophoresis (ULGA-PAGE). Cytosolic fractions from Frankia sp. were electrophoresed at 4 degrees C in the presence of Co2+, Zn2+, or Mg2+ ions. Then
José María Landete et al.
Applied and environmental microbiology, 79(18), 5509-5518 (2013-07-10)
Lactobacillus casei is the only lactic acid bacterium in which two pathways for l-malate degradation have been described: the malolactic enzyme (MLE) and the malic enzyme (ME) pathways. Whereas the ME pathway enables L. casei to grow on l-malate, MLE
D C Simcock et al.
Experimental parasitology, 132(2), 267-273 (2012-08-21)
Nematodes which have adapted to an anaerobic lifestyle in their adult stages oxidise phosphoenolpyruvate (PEP) to oxaloacetate rather than pyruvate as the final product of glycolysis. This adaptation involves selective expression of the enzyme phosphoenolpyruvate carboxykinase (PEPCK), instead of pyruvate
A Ruhal et al.
Cellular and molecular biology (Noisy-le-Grand, France), 58(1), 15-20 (2013-01-01)
An amperometric malic acid biosensor was developed by immobilizing malate dehydrogenase on multi-walled carbon nanotubes (MWCNT) coated on screen printed carbon electrode. The screen printed carbon electrode is made up of three electrodes viz., carbon as working, platinum as counter
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