Showing 1-30 of 96 results for "


Alexis J Haas et al.
Cell reports, 32(3), 107924-107924 (2020-07-23)
Tight-junction-regulated actomyosin activity determines epithelial and endothelial tension on adherens junctions and drives morphogenetic processes; however, whether or not tight junctions themselves are under tensile stress is not clear. Here, we use a tension sensor based on ZO-1, a scaffolding...
Natsuko Izumi et al.
Cancer science, 103(1), 50-57 (2011-09-29)
Heat shock protein 90 (Hsp90), a conserved molecular chaperone for a specific set of proteins critical for signal transduction including several oncogenic proteins, has been recognized as a promising target for anticancer therapy. Hsp90 inhibition also sensitizes cancer cells to...
Epithelial relaxation mediated by the myosin phosphatase regulator Mypt1 is required for brain ventricle lumen expansion and hindbrain morphogenesis
Gutzman JH and Sive H
Development, 137(5), 795-804 (2010)
Venkaiah Betapudi et al.
Journal of molecular biology, 407(5), 673-686 (2011-02-15)
Myosin II motor proteins play important roles in cell migration. Although myosin II filament assembly plays a key role in the stabilization of focal contacts at the leading edge of migrating cells, the mechanisms and signaling pathways regulating the localized...
Kenneth Wee et al.
Journal of cell science, 133(13) (2020-05-30)
Cell extrusion is a morphogenetic process that is implicated in epithelial homeostasis and elicited by stimuli ranging from apoptosis to oncogenic transformation. To explore whether the morphogenetic transcription factor Snail (SNAI1) induces extrusion, we inducibly expressed a stabilized Snail6SA transgene...
Mabel Lum et al.
Pathogens and disease, 72(3), 174-187 (2014-07-06)
A key feature of Shigella pathogenesis is the ability to spread from cell-to-cell post-invasion. This is dependent on the bacteria's ability to initiate de novo F-actin tail polymerisation, followed by protrusion formation, uptake of bacteria-containing protrusion and finally, lysis of...
Armen Petrosyan et al.
The Journal of biological chemistry, 290(10), 6256-6269 (2015-01-22)
Core 2 N-acetylglucosaminyltransferase 2/M (C2GnT-M) synthesizes all three β6GlcNAc branch structures found in secreted mucins. Loss of C2GnT-M leads to development of colitis and colon cancer. Recently we have shown that C2GnT-M targets the Golgi at the Giantin site and...
Eric M Balzer et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 26(10), 4045-4056 (2012-06-19)
Cell migration on planar surfaces is driven by cycles of actin protrusion, integrin-mediated adhesion, and myosin-mediated contraction; however, this mechanism may not accurately describe movement in 3-dimensional (3D) space. By subjecting cells to restrictive 3D environments, we demonstrate that physical...
Yosuke Funato et al.
Developmental cell, 55(4), 387-397 (2020-09-13)
Extracellular pH is usually maintained around 7.4 in multicellular organisms, and cells are optimized to proliferate under this condition. Here, we find cells can adapt to a more acidic pH of 6.5 and become addicted to this acidic microenvironment by...
Nisha Bte Mohd Rafiq et al.
Nature materials, 18(6), 638-649 (2019-05-23)
The interrelationship between microtubules and the actin cytoskeleton in mechanoregulation of integrin-mediated adhesions is poorly understood. Here, we show that the effects of microtubules on two major types of cell-matrix adhesion, focal adhesions and podosomes, are mediated by KANK family...
Hitomi Nakashima et al.
Biophysics and physicobiology, 12, 21-29 (2015-01-01)
Cell-crawling migration plays an essential role in complex biological phenomena. It is now generally believed that many processes essential to such migration are regulated by microtubules in many cells, including fibroblasts and neurons. However, keratocytes treated with nocodazole, which is...
Masaki Tsuchiya et al.
Nature communications, 9(1), 2049-2049 (2018-05-26)
Myotube formation by fusion of myoblasts and subsequent elongation of the syncytia is essential for skeletal muscle formation. However, molecules that regulate myotube formation remain elusive. Here we identify PIEZO1, a mechanosensitive Ca2+ channel, as a key regulator of myotube...
The role of pulmonary vascular contractile protein expression in pulmonary arterial hypertension
Konik EA, et al.
Journal of Molecular and Cellular Cardiology, 65, 147-155 (2013)
Armen Petrosyan et al.
Molecular cancer research : MCR, 12(12), 1704-1716 (2014-08-03)
Prostate cancer progression is associated with upregulation of sialyl-T antigen produced by β-galactoside α-2,3-sialyltransferase-1 (ST3Gal1) but not with core 2-associated polylactosamine despite expression of core 2 N-acetylglucosaminyltransferase-L (C2GnT-L/GCNT1). This property allows androgen-refractory prostate cancer cells to evade galectin-1 (LGALS1)-induced apoptosis...
Lingqing Dong et al.
Biomaterials, 222, 119456-119456 (2019-09-03)
Geometry sensing of cells inevitably involves cytoskeletal remodeling and the activation of biochemical signaling, which control multiple aspects of cell behaviors, such as proliferation, differentiation and migration. A variety of size-, shape- and geometry-dependent cell behaviors have been revealed, but...
Adhesion-dependent and contractile ring-independent equatorial furrowing during cytokinesis in mammalian cells
Kanada M, et al.
Molecular Biology of the Cell, 16(8), 3865-3872 (2005)
Keri B Sanborn et al.
Blood, 118(22), 5862-5871 (2011-11-30)
Natural killer (NK) cells are innate immune lymphocytes that provide critical defense against virally infected and transformed cells. NK-cell cytotoxicity requires the formation of an F-actin rich immunologic synapse (IS), as well as the polarization of perforin-containing lytic granules to...
Wenwu Zhang et al.
The Journal of physiology, 595(13), 4279-4300 (2017-03-18)
Non-muscle (NM) and smooth muscle (SM) myosin II are both expressed in smooth muscle tissues, however the role of NM myosin in SM contraction is unknown. Contractile stimulation of tracheal smooth muscle tissues stimulates phosphorylation of the NM myosin heavy...
Laura Andrés-Delgado et al.
Development (Cambridge, England), 146(13) (2019-06-09)
The epicardium, the outer mesothelial layer enclosing the myocardium, plays key roles in heart development and regeneration. During embryogenesis, the epicardium arises from the proepicardium (PE), a cell cluster that appears in the dorsal pericardium (DP) close to the venous...
Nehal Dwivedi et al.
Biochemical and biophysical research communications, 543, 38-44 (2021-01-29)
Survival and maintenance of normal physiological functions depends on continuous interaction of cells with its microenvironment. Cells sense the mechanical properties of underlying substrate by applying force and modulate their behaviour in response to the resistance offered by the substrate....
Jordan R Beach et al.
The Journal of biological chemistry, 284(40), 27377-27383 (2009-08-08)
During cell division, the mechanisms by which myosin II is recruited to the contractile ring are not fully understood. Much recent work has focused on a model in which spatially restricted de novo filament assembly occurs at the cell equator...
Nisha Bte Mohd Rafiq et al.
The Journal of cell biology, 216(1), 181-197 (2016-12-23)
Podosomes represent a class of integrin-mediated cell-matrix adhesions formed by migrating and matrix-degrading cells. We demonstrate that in macrophage-like THP1 cells and fibroblasts stimulated to produce podosomes, down-regulation of the G-protein ARF1 or the ARF1 guanine nucleotide exchange factor, ARNO...
Thomas Daubon et al.
Journal of cell science, 129(13), 2586-2598 (2016-05-28)
Podosomes are dynamic cell-matrix contact structures that combine several key abilities, including adhesion, matrix degradation and mechanosensing. These actin-based cytoskeletal structures have been mostly studied in monocytic cells, but much less is known about those formed in other lineages. In...
Joe Swift et al.
Science (New York, N.Y.), 341(6149), 1240104-1240104 (2013-08-31)
Tissues can be soft like fat, which bears little stress, or stiff like bone, which sustains high stress, but whether there is a systematic relationship between tissue mechanics and differentiation is unknown. Here, proteomics analyses revealed that levels of the...
Nayana Tusamda Wakhloo et al.
Biomaterials, 234, 119746-119746 (2020-01-17)
Cell deformation occurs in many critical biological processes, including cell extravasation during immune response and cancer metastasis. These cells deform the nucleus, their largest and stiffest organelle, while passing through narrow constrictions in vivo and the underlying mechanisms still remain...
Yuan-Chen Chang et al.
Molecular biology of the cell, 19(5), 2147-2153 (2008-02-22)
The RhoA GTPase plays a vital role in assembly of contractile actin-myosin filaments (stress fibers) and of associated focal adhesion complexes of adherent monolayer cells in culture. GEF-H1 is a microtubule-associated guanine nucleotide exchange factor that activates RhoA upon release...
Chika Okimura et al.
Scientific reports, 8(1), 10615-10615 (2018-07-19)
Crawling migration plays an essential role in a variety of biological phenomena, including development, wound healing, and immune system function. Keratocytes are wound-healing cells in fish skin. Expansion of the leading edge of keratocytes and retraction of the rear are...
Claudia Schäfer et al.
Cell adhesion & migration, 4(2), 215-225 (2010-02-25)
Migration of cells is one of the most essential prerequisites to form higher organisms and depends on a strongly coordinated sequence of processes. Early migratory events include substrate sensing, adhesion formation, actin bundle assembly and force generation. While substrate sensing...
Serge Mostowy et al.
Cell host & microbe, 8(5), 433-444 (2010-11-16)
Actin-based motility is used by various pathogens for dissemination within and between cells. Yet host factors restricting this process have not been identified. Septins are GTP-binding proteins that assemble as filaments and are essential for cell division. However, their role...
Luca Musante et al.
Scientific reports, 10(1), 3701-3701 (2020-03-01)
Urinary extracellular vesicles (uEVs) provide bio-markers for kidney and urogenital diseases. Centrifugation is the most common method used to enrich uEVs. However, a majority of studies to date have focused on the ultracentrifugation pellet, potentially losing a novel source of...