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Shiori Ashibe et al.
Theriogenology, 133, 71-78 (2019-05-10)
Intracytoplasmic sperm injection (ICSI) is an essential technology in animal and human reproduction. However, the developmental competence and pregnancy rate of embryos derived from ICSI are still lower than that from the conventional in vitro fertilization technique. In this report, we...
Matthew D Dean et al.
Evolution; international journal of organic evolution, 63(1), 20-28 (2008-08-30)
Barriers to gene flow can arise at any stage in the reproductive sequence. Most studies of reproductive isolation focus on premating or postzygotic phenotypes, leaving the importance of differences in fertilization rate overlooked. Two closely related species of house mice...
Radu C Zamfirescu et al.
American journal of physiology. Cell physiology, 320(1), C30-C44 (2020-10-15)
Development of the mammalian preimplantation embryo is influenced by autocrine/paracrine factors and the availability of nutrients. Deficiencies of these during in vitro culture reduce the success of assisted reproductive technologies. The mechanistic target of rapamycin complex 1 (mTORC1) pathway integrates...
Florian Bilz et al.
Neuron, 106(6), 963-976 (2020-04-09)
During associative conditioning, animals learn which sensory cues are predictive for positive or negative conditions. Because sensory cues are encoded by distributed neurons, one has to monitor plasticity across many synapses to capture how learned information is encoded. We analyzed...
Danielle Vaughan et al.
European journal of translational myology, 30(2), 8904-8904 (2020-08-13)
Duchenne Muscular Dystrophy is a devastating disease caused by the absence of a functional rod-shaped cytoplasmic protein called dystrophin. Several avenues are being developed aimed to restore dystrophin expression in boys affected by this X-linked disease. However, its complete cure...
Lopa M Das et al.
Autophagy, 15(5), 813-826 (2019-01-22)
Cutaneous inflammation from UV radiation exposure causes epidermal damage, cellular infiltration, and secretion of pro-inflammatory mediators that exacerbate tissue destruction. Recovery is mediated chiefly by anti-inflammatory M2 macrophages that suppress inflammation and augment epidermal regeneration. Vitamin D enables anti-inflammation to...
Mariana Schroeder et al.
Nature communications, 9(1), 1596-1596 (2018-04-25)
Anorexia nervosa (AN) is a devastating eating disorder characterized by self-starvation that mainly affects women. Its etiology is unknown, which impedes successful treatment options leading to a limited chance of full recovery. Here, we show that gestation is a vulnerable...
E Casser et al.
Molecular human reproduction, 25(11), 729-744 (2019-09-11)
It is widely held that the first two blastomeres of mammalian embryos are equally totipotent and that this totipotency belongs to the group of regulative properties. However, this interpretation neglects an important aspect: evidence only came from successful monozygotic twins...
Dale C Brunelle et al.
G3 (Bethesda, Md.), 7(11), 3559-3570 (2017-10-06)
We have previously identified
Gungun Lin et al.
Microfluidics and nanofluidics, 19(2), 457-464 (2015-09-18)
We apply the technique of supervised discriminant analysis (SDA) for in-flow detection in droplet-based magnetofluidics. Based on the SDA, we successfully discriminate
Luiz Fernando Feres et al.
Animal reproduction science, 193, 165-170 (2018-04-22)
The aim of the present study was to evaluate the likelihood of pregnancy of in vitro-produced (IVP) embryos from batches with distinct relative efficiencies. Data were retrospectively analyzed from 605 transvaginal ultrasonic-guided follicle aspiration sessions (OPU) followed by in vitro...
TIE: A Method to Electroporate Long DNA Templates into Preimplantation Embryos for CRISPR-Cas9 Gene Editing
Bagheri H, et al.
The CRISPR journal, 1(3), 223-229 (2018)
G Arrabito et al.
Lab on a chip, 16(24), 4666-4676 (2016-11-05)
The assembly of scalable liquid compartments for binding assays in array formats constitutes a topic of fundamental importance in life sciences. This challenge can be addressed by mimicking the structure of cellular compartments with biological native conditions. Here, inkjet printing...
Yulia Gryaznova et al.
The EMBO journal, 40(7), e106797-e106797 (2021-03-02)
Partitioning of the genome in meiosis occurs through two highly specialized cell divisions, named meiosis I and meiosis II. Step-wise cohesin removal is required for chromosome segregation in meiosis I, and sister chromatid segregation in meiosis II. In meiosis I...
Irena Kukavica-Ibrulj et al.
Journal of bacteriology, 190(8), 2804-2813 (2007-12-18)
Pseudomonas aeruginosa chronic lung infections are the major cause of morbidity and mortality in cystic fibrosis (CF) patients. The P. aeruginosa strains PAO1 and PA14 were compared with the Liverpool epidemic strain LESB58 to assess in vivo growth, infection kinetics...
Yang Liu et al.
Lab on a chip, 20(17), 3144-3157 (2020-07-18)
Fast spreading of antimicrobial resistance is now considered a major global health threat. New technologies are required, enabling rapid diagnostics of bacterial infection combined with fast antimicrobial susceptibility testing (AST) for evaluating the efficiency and dosage of antimicrobial compounds in...
Takaya Totsuka et al.
Scientific reports, 10(1), 6556-6556 (2020-04-18)
To study the functional differences between maternal and paternal genomes in mammalian development, embryos with only one parental genome are often used. Androgenetic embryos are produced by the removal of maternal chromosomes before or after fertilization by techniques that require...
Kazuki Tainaka et al.
Cell reports, 24(8), 2196-2210 (2018-08-23)
We describe a strategy for developing hydrophilic chemical cocktails for tissue delipidation, decoloring, refractive index (RI) matching, and decalcification, based on comprehensive chemical profiling. More than 1,600 chemicals were screened by a high-throughput evaluation system for each chemical process. The...
Tiffany Leidy-Davis et al.
Scientific reports, 8(1), 15028-15028 (2018-10-12)
Here, we describe an expansion of the typical DNA size limitations associated with CRISPR knock-in technology, more specifically, the physical extent to which mouse genomic DNA can be replaced with donor (in this case, human) DNA at an orthologous locus...
Bingyan J Wang et al.
Stem cells translational medicine, 9(5), 620-635 (2020-01-01)
Cardiac interstitial cells (CICs) perform essential roles in myocardial biology through preservation of homeostasis as well as response to injury or stress. Studies of murine CIC biology reveal remarkable plasticity in terms of transcriptional reprogramming and ploidy state with important...
Insulin and branched-chain amino acid depletion during mouse preimplantation embryo culture programmes body weight gain and raised blood pressure during early postnatal life
Velazquez M
Biochimica et Biophysica Acta (BBA)-Molecular Basis of Disease, 1864(2), 590-600 (2018)
Guang-Yu Bai et al.
Oncotarget, 8(41), 69863-69873 (2017-10-21)
Somatic cell nuclear transfer is an important technique for life science research, but its efficiency is still extremely low, and most genes that are important during early development, such as X chromosome-linked genes, are not appropriately expressed during this process....
Keke Shao et al.
PloS one, 6(9), e24910-e24910 (2011-09-29)
Aptamers are short RNA or DNA oligonucleotides which can bind with different targets. Typically, they are selected from a large number of random DNA sequence libraries. The main strategy to obtain aptamers is systematic evolution of ligands by exponential enrichment...
Leen Vandaele et al.
Veterinary research, 42, 14-14 (2011-02-15)
Bluetongue virus serotype 8 (BTV-8), which caused an epidemic in ruminants in central Western Europe in 2006 and 2007, seems to differ from other bluetongue serotypes in that it can spread transplacentally and has been associated with an increased incidence...
Diane F Eilerts et al.
Insects, 9(4) (2018-10-27)
Many biological processes and behaviors in mosquitoes display rhythmic patterns, allowing for fine tuning to cyclic environmental conditions. In mosquitoes, vector-host interactions are primarily mediated by olfactory signals. Previous studies have established that, in the malaria vector Anopheles gambiae, rhythmic...
Huarong Wang et al.
Nature communications, 10(1), 5719-5719 (2019-12-18)
It is known that granulosa cells (GCs) mediate gonadotropin-induced oocyte meiosis resumption by releasing EGF-like factors in mammals, however, the detailed molecular mechanisms remain unclear. Here, we demonstrate that luteinizing hormone (LH) surge-induced histone deacetylase 3 (HDAC3) downregulation in GCs...
Mechanical and signaling mechanisms that guide pre-implantation embryo movement.
Flores, et al.
Development, 147 (2021)
Anna Tetkova et al.
Scientific reports, 9(1), 13121-13121 (2019-09-13)
In the absence of transcription, the regulation of gene expression in oocytes is controlled almost exclusively at the level of transcriptome and proteome stabilization, and translation. A subset of maternal transcripts is stored in a translationally dormant state in the...
Carla Danussi et al.
Molecular and cellular biology, 28(12), 4026-4039 (2008-04-16)
Lymphatic-vasculature function critically depends on extracellular matrix (ECM) and on its connections with lymphatic endothelial cells (LECs). However, the composition and the architecture of ECM have not been fully taken into consideration in studying the biology and the pathology of...
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