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Ana P Alessio et al.
Theriogenology, 85(7), 1297-1311 (2016-02-04)
Transposon-mediated transgenesis is a well-established tool for genome modification in small animal models. However, translation of this active transgenic method to large animals warrants further investigations. Here, the piggyBac (PB) and sleeping beauty (SB) transposon systems were assessed for stable
Anna Tetkova et al.
Scientific reports, 9(1), 13121-13121 (2019-09-13)
In the absence of transcription, the regulation of gene expression in oocytes is controlled almost exclusively at the level of transcriptome and proteome stabilization, and translation. A subset of maternal transcripts is stored in a translationally dormant state in the
K I Belousov et al.
Scientific reports, 11(1), 8797-8797 (2021-04-24)
Nowadays droplet microfluidics is widely used to perform high throughput assays and for the synthesis of micro- and nanoparticles. These applications usually require packaging several reagents into droplets and their mixing to start a biochemical reaction. For rapid mixing microfluidic
G Arrabito et al.
Lab on a chip, 16(24), 4666-4676 (2016-11-05)
The assembly of scalable liquid compartments for binding assays in array formats constitutes a topic of fundamental importance in life sciences. This challenge can be addressed by mimicking the structure of cellular compartments with biological native conditions. Here, inkjet printing
Keke Shao et al.
PloS one, 6(9), e24910-e24910 (2011-09-29)
Aptamers are short RNA or DNA oligonucleotides which can bind with different targets. Typically, they are selected from a large number of random DNA sequence libraries. The main strategy to obtain aptamers is systematic evolution of ligands by exponential enrichment
Luiz Fernando Feres et al.
Animal reproduction science, 193, 165-170 (2018-04-22)
The aim of the present study was to evaluate the likelihood of pregnancy of in vitro-produced (IVP) embryos from batches with distinct relative efficiencies. Data were retrospectively analyzed from 605 transvaginal ultrasonic-guided follicle aspiration sessions (OPU) followed by in vitro
Irena Kukavica-Ibrulj et al.
Journal of bacteriology, 190(8), 2804-2813 (2007-12-18)
Pseudomonas aeruginosa chronic lung infections are the major cause of morbidity and mortality in cystic fibrosis (CF) patients. The P. aeruginosa strains PAO1 and PA14 were compared with the Liverpool epidemic strain LESB58 to assess in vivo growth, infection kinetics
Constantine A Simintiras et al.
Proceedings of the National Academy of Sciences of the United States of America, 118(15) (2021-04-21)
Suboptimal uterine fluid (UF) composition can lead to pregnancy loss and likely contributes to offspring susceptibility to chronic adult-onset disorders. However, our understanding of the biochemical composition and mechanisms underpinning UF formation and regulation remain elusive, particularly in humans. To
Bingyan J Wang et al.
Stem cells translational medicine, 9(5), 620-635 (2020-01-01)
Cardiac interstitial cells (CICs) perform essential roles in myocardial biology through preservation of homeostasis as well as response to injury or stress. Studies of murine CIC biology reveal remarkable plasticity in terms of transcriptional reprogramming and ploidy state with important
TIE: A Method to Electroporate Long DNA Templates into Preimplantation Embryos for CRISPR-Cas9 Gene Editing
Bagheri H, et al.
The CRISPR journal, 1(3), 223-229 (2018)
Leen Vandaele et al.
Veterinary research, 42, 14-14 (2011-02-15)
Bluetongue virus serotype 8 (BTV-8), which caused an epidemic in ruminants in central Western Europe in 2006 and 2007, seems to differ from other bluetongue serotypes in that it can spread transplacentally and has been associated with an increased incidence
Astrid Deryckere et al.
eLife, 10 (2021-08-25)
Cephalopods have evolved nervous systems that parallel the complexity of mammalian brains in terms of neuronal numbers and richness in behavioral output. How the cephalopod brain develops has only been described at the morphological level, and it remains unclear where
Yingnan Sun et al.
Lab on a chip, 15(11), 2429-2436 (2015-04-24)
In recent years, inkjet printing, as a new method to fabricate microdroplet microarrays, has been increasingly applied in the field of biochemical diagnostics. To further improve the general applicability of the inkjet printing technology in fabricating biochemical chips, in this
Kazuki Tainaka et al.
Cell reports, 24(8), 2196-2210 (2018-08-23)
We describe a strategy for developing hydrophilic chemical cocktails for tissue delipidation, decoloring, refractive index (RI) matching, and decalcification, based on comprehensive chemical profiling. More than 1,600 chemicals were screened by a high-throughput evaluation system for each chemical process. The
Lutz Menzel et al.
Cell reports, 37(4), 109878-109878 (2021-10-28)
Blood endothelial cells display remarkable plasticity depending on the demands of a malignant microenvironment. While studies in solid tumors focus on their role in metabolic adaptations, formation of high endothelial venules (HEVs) in lymph nodes extends their role to the
Carla Danussi et al.
Molecular and cellular biology, 28(12), 4026-4039 (2008-04-16)
Lymphatic-vasculature function critically depends on extracellular matrix (ECM) and on its connections with lymphatic endothelial cells (LECs). However, the composition and the architecture of ECM have not been fully taken into consideration in studying the biology and the pathology of
Yulia Gryaznova et al.
The EMBO journal, 40(7), e106797-e106797 (2021-03-02)
Partitioning of the genome in meiosis occurs through two highly specialized cell divisions, named meiosis I and meiosis II. Step-wise cohesin removal is required for chromosome segregation in meiosis I, and sister chromatid segregation in meiosis II. In meiosis I
Yang Liu et al.
Lab on a chip, 20(17), 3144-3157 (2020-07-18)
Fast spreading of antimicrobial resistance is now considered a major global health threat. New technologies are required, enabling rapid diagnostics of bacterial infection combined with fast antimicrobial susceptibility testing (AST) for evaluating the efficiency and dosage of antimicrobial compounds in
Takaya Totsuka et al.
Scientific reports, 10(1), 6556-6556 (2020-04-18)
To study the functional differences between maternal and paternal genomes in mammalian development, embryos with only one parental genome are often used. Androgenetic embryos are produced by the removal of maternal chromosomes before or after fertilization by techniques that require
Gungun Lin et al.
Microfluidics and nanofluidics, 19(2), 457-464 (2015-09-18)
We apply the technique of supervised discriminant analysis (SDA) for in-flow detection in droplet-based magnetofluidics. Based on the SDA, we successfully discriminate
Guang-Yu Bai et al.
Oncotarget, 8(41), 69863-69873 (2017-10-21)
Somatic cell nuclear transfer is an important technique for life science research, but its efficiency is still extremely low, and most genes that are important during early development, such as X chromosome-linked genes, are not appropriately expressed during this process.
Eugene Y Kim et al.
Clinical immunology (Orlando, Fla.), 127(1), 98-106 (2008-02-16)
The pathogenesis of T cell-mediated diseases like rheumatoid arthritis (RA) has typically been explained in the context of the Th1-Th2 paradigm: the initiation/propagation by pro-inflammatory cytokines, and downregulation by Th2 cytokines. However, in our study based on the adjuvant-induced arthritis
Emma M Roberts et al.
The Journal of endocrinology, 202(3), 453-462 (2009-07-07)
The apelinergic system, comprised of apelin and its G protein-coupled receptor (APJ; APLNR as given in MGI Database), is expressed within key regions of the central nervous system associated with arginine vasopressin (AVP) synthesis and release as well as in
Long Yang et al.
Journal of neural engineering, 17(1), 016036-016036 (2019-11-16)
Microfabricated multielectrode arrays are widely used for high throughput recording of extracellular neural activity, which is transforming our understanding of brain function in health and disease. Currently there is a plethora of electrode-based tools being developed at higher education and
Insulin and branched-chain amino acid depletion during mouse preimplantation embryo culture programmes body weight gain and raised blood pressure during early postnatal life
Velazquez M
Biochimica et Biophysica Acta (BBA)-Molecular Basis of Disease, 1864(2), 590-600 (2018)
Tiffany Leidy-Davis et al.
Scientific reports, 8(1), 15028-15028 (2018-10-12)
Here, we describe an expansion of the typical DNA size limitations associated with CRISPR knock-in technology, more specifically, the physical extent to which mouse genomic DNA can be replaced with donor (in this case, human) DNA at an orthologous locus
Mathieu Métivier et al.
Current biology : CB, 31(4), 684-695 (2020-12-02)
Proper assembly of mitotic spindles requires microtubule nucleation not only at the centrosomes but also around chromatin. In this study, we found that the Drosophila tubulin-specific chaperone dTBCE is required for the enrichment of tubulin in the nuclear space after
Daiki Tanaka et al.
Molecules (Basel, Switzerland), 26(12) (2021-07-03)
In recent years, research on the application of microdroplets in the fields of biotechnology and chemistry has made remarkable progress, but the technology for the stable generation of single-micrometer-scale microdroplets has not yet been established. In this paper, we developed
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