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Product Information Sheet - M7403
MCDB 153 MEDIUM (M7403) - Product Information Sheet
MCDB 153 MEDIUM
With L-Glutamine and 28 mM HEPES,
Without Sodium Bicarbonate
Product Number M7403
Storage Temperature 2-8°C
Product Description
Data Sheet - KMK2
proliferation of keratinocytes.
Keratinocyte Basal Medium is a modified version of
MCDB 151 and MCDB 104 which contains essential and
non-essential amino acids, vitamins, trace minerals,
organic compounds
Data Sheet - MMK2
fully support
plating and proliferation of melanocytes.
Melanocyte Basal Medium is a modified version of
MCDB 151 and MCDB 104 which contains essential and
non-essential amino acids, vitamins, trace minerals
Data Sheet - FMK2
fully support plating and proliferation of
human dermal fibroblasts.
Fibroblast Basal Medium is a modified version of MCDB
105 which contains essential and non-essential amino
acids, vitamins, trace minerals
Cell Culture Media Filtration - Filter Selection and Sizing
10 psi, except
Viresolve® Barrier filter which was run at 30 psi.
Filter
Amin for 1000 L basal medium
(m2)
Amin for 200 L feed
(m2)
Millipore Express® SHC
(0.5/0.2 µm)
TB055 pET System Manual
from the pLysS plasmid, basal expression levels are elevated in cultures grown to
stationary phase. If the gene is toxic, the addition of 0.5–1% glucose to both liquid medium and agar plates may be necessary
inNovations Newsletter #16
solubility screening
Table 1. RoboPop solubility screening protocol
1. Culture E. coli cells in liquid medium under conditions for target protein production.
2. Add 0.1 culture volume premixed PopCulture
Protein Purification and Detection Tools
used for extraction of proteins from liquid cul-
tures of E. coli without harvesting the cells. Addition of 0.1 culture volume
of PopCulture directly to cells in medium, grown at any scale, efficiently
Hallmarks of Aging
other day medium change and skip weekends!
Mon
Passage
Medium
Change
Medium
Change
4 days between passages 5 days between passages 5 days between passages
Medium
Change
Medium
Change