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MultiScreen HTS Vacuum Manifold
MultiScreenHTS vacuum manifold is
ideal for multiwell filter plate applica-
tions in both manual and automated
laboratory environments. The manifold
supports a wide variety of MultiScreen
platforms, including
User Guide
receiver plate and place the assembly in the base of
the vacuum manifold. The collar fits over the assembly.
See the MultiScreen®HTS Vacuum Manifold User Guide
for details.
NOTE: Optimal vacuum levels
MultiScreen HTS 384 Hydrophilic PVDF Filter Plates
Compatible with existing MultiScreen™HTS vacuum
manifold and its DirectStack™ technology
MultiScreen®HTS
384 Hydrophilic
PVDF Filter Plates
384-well plates incorporate optimized hydrophilic
MultiScreen® and Millicell® Filter Plates for Assay Development
collection plate and reading in a Tecan Spectrafluor™
Plus plate reader.
39
Vacuum Filtration
MultiScreen®HTS Vacuum Manifold
Description Qty/Pk Cat. No.
MultiScreen®HTS vacuum manifold
MultiScreenHTS-PH Phosphocellulose Filter Plates
PC0027EN00: Protein Kinase Assays
on MultiScreen®HTS-PH 384-well Filter
Plates, AN1017EN00: Kinase Assays
Performed Entirely in the
MultiScreenHTS-PH 384-well Filter
Plate, or AN1018EN00: Use of
MultiScreenHTS-PH
MultiScreen® HTS Vacuum Manifold User Guide
15MultiScreen®HTS Vacuum Manifold
Determining Plate Type
The MultiScreen®HTS Vacuum Manifold works well with MultiScreen® filter
plates and a variety of collection plates. This table describes
MultiScreen HTS Glass Fiber Filter Plates
MultiScn HTS 384_PF1150EN00.qxd
MultiScreen®HTS
Glass Fiber Filter Plates
384- and 96-well plates incorporate
optimized glass fiber for assays including
receptor-ligand binding
E Plate designs
User Guide-MVHVN4525|MVFCN1225|MSVMHTSHV|MVCPN0025
Use the MultiScreen® High
Volume Filter Plate
The MultiScreen® High Volume Filter Plate is designed
for use with a MultiScreen®HTS Vacuum Manifold.
Assembly
Refer to the MultiScreen®HTS Vacuum Manifold
User Guide-MSVMHTS0D
MSVMHTS0D 1
MultiScreen®HTS Vacuum Manifold MSVMHTS00 1
MultiScreen® Deep Well Solvinert™ Plate, 0.45 µm, philic, non-sterile MDRLN0410 10
MultiScreen® Deep Well Solvinert™ Plate, 0.45 µm, phobic
Montage Plasmid MiniprepHTS Kit User Guide
clearing plate into the MultiScreen
HTS
PLASMID plate. Discard the MultiScreen
HTS
CLEARING plate.
8. Place the MultiScreen
HTS
PLASMID plate on top of the manifold collar. Apply
vacuum at
MultiScreen HTS 384-Well Plates
liquid into filter plates
■ Vacuum manifold (Millipore cat. no. MSVM HTS 00) or
centrifuge with a swinging bucket rotor and multiwell
plate carrier
■ Solid bottom 384-well assay plates (if collecting
Novel radioligand binding assays with GPCR membranes prefrozen on MultiScreen HTS glass fiber filter plates (Ready-To-Assay plates)
Millipores’s MultiScreen®HTS Filter Plate technology
1. The deep wells of the MultiScreenHTS filter plates permit binding reactions to be
performed in the filter plate
2. Vacuum manifold is used for
MultiScreen HTS PLASMID 96-Well Plates User Guide
from the plate.
Materials Required
In order to use the MultiScreenHTS PLASMID plates you will need:
■ MultiScreenHTS vacuum manifold (Cat. No. MSVM HTS 00)
■ MultiScreenHTS CLEARING plates (Cat. No.
Poster: Automation of Receptor-Ligand Binding Assays using the MultiScreen HTS Filter Plate
a filter plate
RadioLigand Comp. Ligand Receptor
V-Bottom
w/ Stocks:
Tip
Disposal
Chute
Vacuum
Manifold
In-Plate Receptor/Ligand Binding in
MultiScreen® Filter Binding Plates
3.)
Aqueous Solubility Testing with the MultiScreen HTS - PCF Filter Plate
with the
MultiScreen®HTS – PCF Filter Plate
Application note
table 1. comparison of the shake-flask & Multiscreen
solubility tests at pH 7.4
solubility drug shake-Flask Multiscreen
Tamoxifen
Adrenergic receptor family membrane preparation array with Millipore's MultiScreen HTS + Hi Flow 96-well glass fiber filter plates
filter plates and vacuum manifold are designed to be
used with standard automation equipment
Add 0.1% PEI to MultiScreen-FC plate
Filter PEI through plate with vacuum manifold
Wash plate with
MultiScreenHTS Filter Plates: New plate design for full automation compatibility
Solubility filter plate. The 48 adjacent wells received an equivalent volume of PBS. The filter plate
was subsequently placed on a MultiScreen vacuum manifold containing a 96 well collection plate and the samples
Kinase Assays Performed Entirely in the MultiScreen® HTS–PH 384-well Phosphocellulose Filter Plate
Phosphoric acid. Out-of-plate
reactions were transferred from the reaction plate to the prewet filter plate. All filter
plates were washed 5 times under vacuum on a vacuum manifold (Millipore cat#
MSVMHTS00
Automating Radioisotopic Protein Kinase Assays in Phosphocellulose Filter Plates
#14501016). An
entire in-plate assay involving transfer from four 96-well compound plates into a MZPH
filter plate set on a MultiScreenHTS Vacuum Manifold (Millipore #MSVM HTS 00),
excluding incubation
MultiScreen®Filter Plates Poster
with the
MultiScreen®HTS Vacuum Manifold
Plate-on-plate stacking eliminates
gaps between flow directors and
receiver wells in applications that
require filtrate collection.
1. Manifold Collar
MultiScreen Harvest Plates
the addition of MultiScreen
Harvest Plates, Millipore now pro-
vides a complete line of 96-well filter
plates for high throughput screening
(HTS) applications. Fill all your HTS
filter plate needs in one
User Guide - MAHVN4510/MAHVN4550/MSHVN4510/MSHVN4550
MultiScreen®HTS-HV plates are automation-compatible for use with a variety of robotics systems and can
be barcoded on all four sides . These plates should be used with a MultiScreen®HTS Vacuum Manifold
MultiScreen Ultracel®-10 filter plates: Purification of Serum Peptides for Biomarkers Research
reflector mode.
• Millipore, Multiscreen Vacuum Manifold #MAVM0960R
Reagents
• Human serum (Sigma) from clotted male whole blood donor, lot 22K 0965
• Other human serum obtained in-house from healthy
User Guide-MAHVN4510|MAHVN4550|MSHVN4510|MSHVN4550
.
MultiScreen®HTS-HV plates are automation-compatible for use with a variety of robotics systems and
can be barcoded on all four sides. These plates should be used with a MultiScreen®HTS Vacuum Manifold
Use of MultiScreenHTS 384 Filter Plates on the Wallac MicroBeta Trilux Plate Reader to Achieve Optimal Radiometric Assay Results
.doc
Applications Note
Title: Use of MultiScreen®HTS 384 Filter Plates on the Wallac MicroBeta®Trilux
Plate Reader to Achieve Optimal Radiometric Assay Results
Brochure- Sterile Filteration Systems
-sterile-filters
15
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Multiwell Plates
MultiScreen®HTS Filter Plates
Automation-compatible MultiScreen® filter plates that contain a microporous membrane are ideal for clarifying
Sterile Filtration You Trust
-sterile-filters
15
Related Products:
Multiwell Plates
MultiScreen®HTS Filter Plates
Automation-compatible MultiScreen® filter plates that contain a microporous membrane are ideal for clarifying
Multi-SPE Extraction Plates
MSPE NUR 01
Accessories
MultiScreen™HTS Vacuum Manifold 1 MSVM HTS 00
Deep Well Collar 1 MSVM HTS 0D
Deep Well Receiver Plate 50 MDCP N2M 50
Chemical Duty Pump 1
115 volts, 60 Hz WP61 115 60
Brochure-SLTEST000
-sterile-filters
15
Related Products:
Multiwell Plates
MultiScreen®HTS Filter Plates
Automation-compatible MultiScreen® filter plates that contain a microporous membrane are ideal for clarifying
MultiScreen Caco-2 --Drug Transport Assembly in a 96-well system:
pass-
ages 33-35 were used to seed one
MultiScreen Caco-2 plate (96-well
filter plate from cat. MACAC02S5
or MACAC02S2) and four 24-well
plates for 21-day cell growth in four
independent
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