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Sara Bautista-Expósito et al.
Food chemistry, 248, 262-271 (2018-01-14)
Lentil fermentation has a promising potential as a strategy for development of multifunctional ingredients targeting metabolic syndrome (MetS). Response surface methodology was applied to optimize lentil fermentation and study its effects on generation of peptides, soluble phenolics and bioactivities. Fermentation
Qiongju Cao et al.
Food chemistry, 333, 127528-127528 (2020-07-19)
Endogenous lipase and lipoxygenase play important roles in accelerating lipid oxidation. Polyphenols are a series of commonly used chemicals for preserving fish and seafood products, due to their positive inhibitory effects on lipid oxidation. However, the mechanism involved is still
Filipa Gil et al.
Microbiology (Reading, England), 154(Pt 5), 1364-1371 (2008-05-03)
dsDNA bacteriophages use the dual system endolysin-holin to achieve lysis of their bacterial host. In addition to these two essential genes, some bacteriophages encode additional proteins within their lysis module. In this report, we describe the activity of a protein
Changhyun Roh et al.
International journal of molecular sciences, 13(2), 1710-1719 (2012-03-13)
Obesity is a global health problem. It is also known to be a risk factor for the development of metabolic disorders, type 2 diabetes, systemic hypertension, cardiovascular disease, dyslipidemia, and atherosclerosis. In this study, we screened crude extracts from 400
Wenqian Li et al.
Protein expression and purification, 152, 64-70 (2018-04-24)
This paper first describes characterization of two thermostable esterases (ThLip1 and ThLip2) from the thermophilic bacterium Thermoanaerobacterium thermosaccharolyticum DSM 571. The recombinant esterase ThLip1 was active at 80 °C, pH 6.5 and maintained approx. 85% of original activity after 2 h incubation
Xiawei Jiang et al.
Archives of microbiology, 194(3), 207-214 (2011-08-24)
A deep-sea sediment metagenomic library was constructed and screened for lipolytic enzymes by activity-based approach. Nine novel lipolytic enzymes were identified, and the amino acid sequences shared 56% to 84% identity to other lipolytic enzymes in the database. Phylogenetic analysis
Adel Abouhmad et al.
International journal of molecular sciences, 21(9) (2020-05-06)
Mycobacteriophages possess different sets of lytic enzymes for disruption of the complex cell envelope of the mycobacteria host cells and release of the viral progeny. Lysin B (LysB) enzymes are mycolylarabinogalactan esterases that cleave the ester bond between the arabinogalactan
Sonja Heumann et al.
Biotechnology and bioengineering, 102(4), 1003-1011 (2008-10-23)
An alkali stable polyamidase was isolated from a new strain of Nocardia farcinica. The enzyme consists of four subunits with a total molecular weight of 190 kDa. The polyamidase cleaved amide and ester bonds of water insoluble model substrates like
Haliru Musa et al.
3 Biotech, 9(8), 314-314 (2019-08-14)
Initially, a new moderate halophilic strain was locally isolated from seawater. The partial 16S rRNA sequence analysis positioned the organism in Marinobacter genus and was named 'Marinobacter litoralis SW-45'. This study further demonstrates successful utilization of the halophilic M. litoralis
Myung Hwan Lee et al.
Applied microbiology and biotechnology, 88(5), 1125-1134 (2010-08-05)
Soil metagenome constitutes a reservoir for discovering novel enzymes from the unculturable microbial diversity. From three plant rhizosphere metagenomic libraries comprising a total of 142,900 members of recombinant plasmids, we obtained 14 recombinant fosmids that exhibited lipolytic activity. A selected
Sonia Moreno-Perez et al.
Journal of agricultural and food chemistry, 65(1), 117-122 (2016-12-16)
Different immobilized derivatives of two lipases were tested as catalysts of the synthesis of ethyl esters of omega-3 fatty acids during the ethanolysis of sardine oil in solvent-free systems at 25 °C. Lipases from Thermomyces lanuginosus (TLL) and Lecitase Ultra
Renia Fotiadou et al.
Nanomaterials (Basel, Switzerland), 9(6) (2019-05-31)
In the present study, hybrid nanoflowers (HNFs) based on copper (II) or manganese (II) ions were prepared by a simple method and used as nanosupports for the development of effective nanobiocatalysts through the immobilization of lipase B from Pseudozymaantarctica. The
L Costa et al.
Applied spectroscopy, 62(8), 932-935 (2008-08-16)
The kinetics of the hydrolysis reaction of p-nitrophenyl butyrate catalyzed by a single-site mutant of Fusarium solani pisi cutinase, supported on zeolite NaY by adsorption, was followed using ultraviolet-visible (UV/Vis) in situ diode array spectrophotometry. Spectral band-fitting techniques allowed the
Pan Wang et al.
BMC microbiology, 20(1), 262-262 (2020-08-26)
Glycosylphosphatidylinositol (GPI)-anchored glycoproteins have diverse intrinsic functions in yeasts, and they also have different uses in vitro. In this study, the functions of potential GPI proteins in Pichia pastoris were explored by gene knockout approaches. Through an extensive knockout of
Daniel Grajales et al.
New biotechnology, 47, 25-30 (2018-02-14)
Protein immobilization is a key enabling technology for flow biocatalysis. For this purpose, many different immobilization protocols and characterization techniques have been developed in recent decades. However, examples where proteins are directly immobilized on ready-to-use reactors are scarce, likely due
Meiling Chen et al.
Biotechnology letters, 42(1), 171-180 (2019-11-21)
To identify novel lipases with stability and long-chain fatty acids preference by phylogenetic evolution analysis methods from database. Thermo-stable Candida antarctica Lipase-A (CALA) was set as a template for gene mining by PSI-BLAST. Based on phylogenetic analysis, three candidate lipases
Sabrina Ait Braham et al.
Molecules (Basel, Switzerland), 26(4) (2021-03-07)
This paper aims to investigate the effects of some salts (NaCl, (NH4)2SO4 and Na2SO4) at pH 5.0, 7.0 and 9.0 on the stability of 13 different immobilized enzymes: five lipases, three proteases, two glycosidases, and one laccase, penicillin G acylase
Haibo Xu et al.
Food chemistry, 188, 439-445 (2015-06-05)
An acidic cutinase (TtcutB) from Thielavia terrestris CAU709 was purified to apparent homogeneity with 983 Um g(-1) specific activity. The molecular mass of the enzyme was estimated to be 27.3 and 27.9 kDa by SDS-PAGE and gel filtration, respectively. A
Alejandro H Orrego et al.
International journal of molecular sciences, 19(2) (2018-02-15)
Immobilized enzymes have a very large region that is not in contact with the support surface and this region could be the target of new stabilization strategies. The chemical amination of these regions plus further cross-linking with aldehyde-dextran polymers is
Jiao Tang et al.
Applied microbiology and biotechnology, 103(7), 3037-3048 (2019-02-15)
The thermophilic fungus Thielavia terrestris when cultured on cellulose produces a cocktail of thermal hydrolases with potential application in saccharification of lignocellulosic biomass and other biotechnological areas. Glucuronoyl esterases are considered to play a unique role as accessory enzymes in
Deise Juliana Kolling et al.
Applied biochemistry and biotechnology, 163(2), 304-312 (2010-07-24)
A recombinant esterase from Lactobacillus plantarum was immobilized on hydrophobic support polypropylene Accurel MP1000 by adsorption. Adsorption efficiency was 83%, and the immobilized protein was 12.4 mg/g of support. Esterase activity was determined using p-nitrophenyl butyrate as substrate, and highest
Nattapon Supkamonseni et al.
Indian journal of experimental biology, 52(10), 965-971 (2014-10-28)
In vitro study revealed that pancreatic lipase inhibitory activity of C. asiatica extract was significantly higher than rutin but lower than orlistat, an anti-obesity drug. alpha-Amylase inhibitory activities of C. asiatica extract and rutin were significantly lower than acarbose, an
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Joanne L Porter et al.
ACS chemical biology, 10(2), 611-621 (2014-11-25)
The ease with which enzymes can be adapted from their native roles and engineered to function specifically for industrial or commercial applications is crucial to enabling enzyme technology to advance beyond its current state. Directed evolution is a powerful tool
Eric T Williams et al.
Drug metabolism letters, 5(4), 280-289 (2011-10-26)
Rufinamide was evaluated in vitro to determine which enzyme(s) are responsible for rufinamide hydrolysis and whether valproate, one of its metabolites (valproyl-CoA), and/or the rufinamide hydrolysis product (CGP 47292) could inhibit hydrolysis. Rufinamide hydrolysis was mediated primarily by human carboxylesterase
Hao Zhou et al.
Biotechnology letters, 34(6), 1107-1113 (2012-03-01)
A C-C hydrolase gene (bphD(LA-4)) from strain Dyella ginsengisoli LA-4 was cloned and expressed in Escherichia coli BL21 (DE3). BphD(LA-4) together with another hydrolase MfphA(LA-4), which derived from the same strain, possessed esterase activities. p-Nitrophenyl butyrate was the best substrate
Li-Chun Wang et al.
Journal of food and drug analysis, 26(3), 973-984 (2018-07-07)
Obesity is associated with higher risks of developing diabetes and cardiovascular disease. Green tea, rich in polyphenolic compounds such as epigallocatechin gallate (EGCG) and epigallocatechin (EGC), has been shown to display anti-obesity effects. Houttuynia cordata leaves have also been shown
Chan Hee Lee et al.
Frontiers in bioengineering and biotechnology, 8, 553591-553591 (2020-11-10)
Biomineralized uniform and well-organized calcium carbonate microspheres were synthesized for enzyme immobilization, and the immobilized enzyme was successfully stabilized. The physicochemical parameters of calcium carbonate were studied using scanning electron microscopy with energy-dispersive X-ray spectroscopy, particle size analysis, X-ray diffraction
Justin J Miller et al.
eLife, 10 (2021-07-20)
Carboxy ester prodrugs are widely employed to increase oral absorption and potency of phosphonate antibiotics. Prodrugging can mask problematic chemical features that prevent cellular uptake and may enable tissue-specific compound delivery. However, many carboxy ester promoieties are rapidly hydrolyzed by
Jiaming Yang et al.
Frontiers in bioengineering and biotechnology, 8, 544058-544058 (2020-11-17)
A cell surface displayed system in Pichia pastoris GS115 was developed by using GCW61, a glycosylphosphatidylinositol-modified cell wall protein from P. pastoris, as the anchor protein. Thermomyces lanuginosus lipase (TLL) was successfully displayed on the P. pastoris cell wall by
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