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Tomer Shpilka et al.
The EMBO journal, 34(16), 2117-2131 (2015-07-15)
Autophagy is a major catabolic process responsible for the delivery of proteins and organelles to the lysosome/vacuole for degradation. Malfunction of this pathway has been implicated in numerous pathological conditions. Different organelles have been found to contribute to the formation
Hang Qi et al.
Biochimica et biophysica acta, 1810(7), 704-712 (2011-05-14)
Phloxine B (PhB; 2',4',5',7'-tetrabromo-4,5,6,7-tetrachloro-fluorescein), an artificial xanthene colorant, has been used as a red coloring agent in drugs and cosmetics as well as foods in some countries. However, little effort has been devoted to the study of this colorant as
Jae Su Kim et al.
Pest management science, 66(12), 1337-1343 (2010-08-19)
Insecticidal activities of five photoactive dyes against greenhouse whitefly (GWF), Trialeurodes vaporariorum West., in tomatoes were investigated to improve the control efficacy of an entomopathogenic fungal product, Isaria fumosorosea SFP-198 wettable powder (WP). Azorubine, Eosin B, Erythrosine, Brilliant Green and
Ellyn E Ritchie et al.
Chemosphere, 90(7), 2129-2135 (2012-12-06)
Soil eco-toxicity testing was conducted in support of Canada's Chemical Management Plan (CMP) to fill data gaps for organic chemicals known to primarily partition to soil, and of which the persistence and inherent toxicity are uncertain. Two compounds representative of
Xiaoli Song et al.
Luminescence : the journal of biological and chemical luminescence, 28(1), 16-22 (2012-02-23)
We report for the first time that the sensitivity of the luminol-hypochlorite chemiluminescence (CL) reaction was enhanced approximately 10 times by the addition of phloxine B. The maximum wavelength of CL emission shifted from 431 to 595 nm in the
Adrian Weisz et al.
Journal of chromatography. A, 1113(1-2), 186-190 (2006-02-24)
The present work describes (a) the identification and characterization of an impurity, 2,4,5,7-tetrabromo-6-hydroxy-9-(2,3,4,5-tetrachlorophenyl)-3H-xanthen-3-one (BCPX), in the color additives D&C Red Nos. 27 and 28 (phloxine B) and (b) the determination of the extent and level of BCPX contamination in certified
Guobo Guan et al.
PLoS biology, 17(3), e2006966-e2006966 (2019-03-14)
While sexual reproduction is pervasive in eukaryotic cells, the strategies employed by fungal species to achieve and complete sexual cycles is highly diverse and complex. Many fungi, including Saccharomyces cerevisiae and Schizosaccharomyces pombe, are homothallic (able to mate with their
Advances in measuring lifespan in the yeast Saccharomyces cerevisiae
Nadege Minios
Proceedings of the National Academy of Sciences of the USA (2004)
Ander M de Lecea et al.
Rapid communications in mass spectrometry : RCM, 25(13), 1853-1861 (2011-06-04)
Stable isotope measurements are an important tool for ecosystem trophic linkage studies. Ideally, fresh samples should be used for isotopic analysis, but in many cases organisms must be preserved and analysed later. In some cases dyes must be used to
Hang Qi et al.
Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association, 50(6), 1841-1847 (2012-03-24)
We investigated the molecular mechanisms underlying phloxine B (PhB)-induced photocytotoxicity in human T lymphocytic leukemia Jurkat cells. In addition to apoptosis-related biochemical events, photo-irradiated PhB generated intracellular reactive oxygen species (ROS), induced phosphorylation of c-Jun-N-terminal kinase (JNK) in an oxidative
Paul M van Midwoud et al.
Analytical chemistry, 83(1), 84-91 (2010-12-07)
A novel approach for on-line monitoring of drug metabolism in continuously perifused, precision-cut liver slices (PCLS) in a microfluidic system has been developed using high-performance liquid chromatography with UV detection (HPLC-UV). In this approach, PCLS are incubated in a microfluidic
Masashi Yukawa et al.
G3 (Bethesda, Md.), 9(1), 269-280 (2018-11-23)
The Kinesin-5 motor Cut7 in Schizosaccharomyces pombe plays essential roles in spindle pole separation, leading to the assembly of bipolar spindle. In many organisms, simultaneous inactivation of Kinesin-14s neutralizes Kinesin-5 deficiency. To uncover the molecular network that counteracts Kinesin-5, we
Pierre Carpentier et al.
Neurotoxicology, 33(4), 789-795 (2011-12-14)
The Woelcke method is classically used for myelin staining. Degenerating neurons can be revealed histologically by hemalun and phloxin (H&P) where they appear "eosinophilic". In the first 24 h following soman-induced status epilepticus, we observed that the Woelcke method also
Phloxine B, a versatile bacterial stain
Reuven Rrasooly
Pathogens and Disease (2007)
Reuven Rasooly
FEMS immunology and medical microbiology, 49(2), 261-265 (2007-03-03)
The data presented suggest that Phloxine B, a color additive for food, drugs, and cosmetics has a potential use as a nontoxic, faster (<2 min), inexpensive (350 tests for <1 cent material) and simpler to use alternative to Gram staining.
Andreas Kortekamp
Mycological research, 109(Pt 5), 640-648 (2005-07-16)
Although Plasmopara viticola causes grape downy mildew in most, if not all, wine producing countries, many basic biological or chemical aspects are still unknown and thus the histopathological changes during development of this oomycete pathogen were studied. The fluorochromes Aniline
J R Walton
Neurotoxicology, 30(6), 1059-1069 (2009-07-14)
A recent longitudinal study described an inducible rodent model for age-related cognitive deterioration. This model was produced by chronically feeding rats aluminum, from age 12 months onwards, in measured amounts equivalent to total aluminum levels ingested by Americans from their
Avraham Rasooly et al.
Antimicrobial agents and chemotherapy, 46(11), 3650-3653 (2002-10-18)
Fluorescein dyes in which the benzoic acid moiety has been tetrachlorinated (50 to 100 micro g/ml) inhibit in vitro Staphylococcus aureus growth (MIC, 25 micro g/ml). Specifically, under standard room illumination, phloxine B at a concentration of 100 micro g/ml
David G Bole et al.
Neurochemical research, 30(3), 363-369 (2005-07-16)
Synaptic vesicular accumulation of glutamate is a vital initial step in glutamate transmission. We have previously shown that Rose Bengal, a polyhalogenated fluorescein analog, is a potent inhibitor of glutamate uptake into synaptic vesicles. Here, we report the structural features
Stephen D Willis et al.
Molecular biology of the cell, 31(10), 1015-1031 (2020-03-12)
Environmental stress elicits well-orchestrated programs that either restore cellular homeostasis or induce cell death depending on the insult. Nutrient starvation triggers the autophagic pathway that requires the induction of several Autophagy (ATG) genes. Cyclin C-cyclin-dependent kinase (Cdk8) is a component
Maged Fouad et al.
Electrophoresis, 29(11), 2280-2287 (2008-05-01)
We describe a new and selective analytical method for the separation and quantitation of plant glucosinolates. The new method, which utilizes microchip CE (micro-CE) with fluorescence detection, circumvents the multistep procedures characteristic of conventional methods. Glucosinolates form charge transfer complexes
Olatz Albaina et al.
Journal of medical microbiology, 64(Pt 2), 147-156 (2014-12-07)
Candida dubliniensis is a pathogenic yeast of the genus Candida closely related to Candida albicans. The phenotypic similarity of these two species often leads to misidentification of C. dubliniensis isolates in clinical samples. DNA-based methods continue to be the most
Takeshi Noda
Methods in enzymology, 451, 27-32 (2009-02-03)
In the yeast Saccharomyces cerevisiae, autophagy contributes to the sustaining of cell viability under starvation conditions, possibly through the supply of amino acids that is generated as a result of the degradation of cytosolic materials. Therefore, cellular viability is one
Hong Wang et al.
Biomedical and environmental sciences : BES, 21(5), 438-441 (2009-01-13)
To determine the visible light-induced photodegradation kinetics of two xanthene photosensitizers, phloxine B and uranine, in solution and on the surface of silica TLC plates, and to examine the phototoxicity of residues of degradation, which could provide valuable safety data
Norihiro Mutoh et al.
Yeast (Chichester, England), 22(2), 91-97 (2005-01-13)
A Schizosaccharomyces pombe mutant deficient in Cu,Zn-superoxide dismutase (sod1 mutant) was hypersensitive to phloxine B, which is used as a food-colouring agent and also to distinguish diploid strains of Sz. pombe from haploid strains, under illumination with light. The pro-oxidant
Reuven Rasooly
FEMS immunology and medical microbiology, 45(2), 239-244 (2005-06-14)
Phloxine B (D&C red no. 28) is a color additive for food, drugs, and cosmetics. It has been previously shown to have anti-Staphylococcus aureus activities. In this work, the effect of Phloxine B on various gram-negative bacteria and other gram-positive
Patricia Melin et al.
The Journal of membrane biology, 208(3), 203-212 (2006-04-11)
The cystic fibrosis transmembrane conductance regulator (CFTR) protein contains a canonical ATP-binding cassette (ABC) signature motif, LSGGQ, in nucleotide binding domain 1 (NBD1) and a degenerate LSHGH in NBD2. Here, we studied the contribution of the conserved residues G551 and
Using Vital Dyes to Trace Uptake of dsRNA by Green Peach Aphid Allows Effective Assessment of Target Gene Knockdown
International Journal of Molecular Sciences (2017)
Grant T McQuate et al.
Journal of economic entomology, 98(4), 1170-1178 (2005-09-15)
Spinosad and phloxine B are two more environmentally friendly alternative toxicants to malathion for use in bait sprays for tephritid fruit fly suppression or eradication programs. Laboratory tests were conducted to assess the relative toxicity of these two toxicants for
Yuichiro Maeda et al.
Autophagy, 11(8), 1247-1258 (2015-06-11)
Lipid droplets (LDs) are the conserved organelles for the deposit of neutral lipids, and function as reservoirs of membrane and energy sources. To date, functional links between autophagy and LD dynamics have not been fully elucidated. Here, we report that
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