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Swetha Reddy et al.
Bio-protocol, 7(9), e2267-e2267 (2017-05-05)
Listeria monocytogenes is an important Gram-positive foodborne pathogen that is a particular problem in ready-to-eat food. It has an ability to survive in harsh conditions like refrigeration temperatures and high salt concentrations and is known to cross intestinal, placental and
Loreen R Stromberg et al.
Mikrochimica acta, 186(8), 533-533 (2019-07-17)
A multi-step approach is described for the fabrication of multi-layer graphene-based electrodes without the need for ink binders or post-print annealing. Graphite and nanoplatelet graphene were chemically exfoliated using a modified Hummers' method and the dried material was thermally expanded.
Jinzhi Li et al.
Cell reports, 32(11), 108138-108138 (2020-09-17)
The patterns of neuronal connectivity underlying multisensory integration, a fundamental property of many brains, remain poorly characterized. The Drosophila melanogaster mushroom body-an associative center-is an ideal system to investigate how different sensory channels converge in higher order brain centers. The
Sara López-Martínez et al.
Frontiers in bioengineering and biotechnology, 9, 639688-639688 (2021-03-23)
Decellularization techniques support the creation of biocompatible extracellular matrix hydrogels, providing tissue-specific environments for both in vitro cell culture and in vivo tissue regeneration. We obtained endometrium derived from porcine decellularized uteri to create endometrial extracellular matrix (EndoECM) hydrogels. After
Mark E Warchol et al.
Developmental neurobiology, 69(2-3), 191-202 (2009-01-09)
The paired-domain transcription factor Pax2 is involved in many facets of inner ear development, but relatively little is known about the expression or function of Pax2 in the mature ear. In this study, we have used immunohistochemical methods to characterize
Xuan Ju et al.
Frontiers in psychiatry, 12, 599399-599399 (2021-08-03)
Background: Rapid eye movement sleep deprivation (REMSD) and fluoxetine affect depression, yet the detailed molecular mechanisms were not clear. Methods: Rat depression chronic unpredictable stress was constructed, and the body weight of rats was measured. The efficacy of REMSD and
Margit Mutso et al.
Bio-protocol, 9(12), e3282-e3282 (2019-06-20)
Virus-generated PAMP RNAs are key factors that activate host immune response. The PAMP RNAs are therefore usually closely related with viral disease pathogenesis. Quantitative real time PCR is a conventional method to assess RNA. However, it cannot be used for
Aleksandra Glogowska et al.
Neoplasia (New York, N.Y.), 10(10), 1120-1130 (2008-09-25)
The intracellular domains of the membrane-anchoring regions of some precursors of epidermal growth factor (EGF) family members have intrinsic biologic activities. We have determined the role of the human proEGF cytoplasmic domain (proEGFcyt) as part of the proEGF transmembrane-anchored region
Ryan Marshall et al.
STAR protocols, 1(1), 100003-100003 (2020-10-29)
We present a protocol to rapidly test DNA binding and cleavage activity by CRISPR nucleases using cell-free transcription-translation (TXTL). Nuclease activity is assessed by adding DNA encoding a nuclease, a guide RNA, and a targeted reporter to a TXTL reaction
Abir O El Sadik et al.
PloS one, 10(9), e0137544-e0137544 (2015-09-25)
Wound healing involves the integration of complex biological processes. Several studies examined numerous approaches to enhance wound healing and to minimize its related morbidity. Both chitosan and mesenchymal stem cells (MSCs) were used in treating skin wounds. The aim of
Shiwei Xie et al.
International journal of biological sciences, 17(9), 2367-2379 (2021-07-10)
Recombinant human bone morphogenetic proteins (rhBMPs) can stimulate bone formation and growth in the treatment of spinal fusions and nonunions. However, it is still unclear whether rhBMPs function in the prevention of intervertebral disc degeneration (IDD). Here, we discovered that
Mustafa S Atta et al.
Oxidative medicine and cellular longevity, 2018, 7845681-7845681 (2018-12-05)
Diabetic cardiomyopathy is a diabetic complication due to oxidative stress injuries. This study examined the protecting influence of thymoquinone (TQ) on diabetes-caused cardiac complications. The intracellular means by which TQ works against diabetes-caused cardiac myopathy in rats is not completely
Minghua Yang et al.
Autophagy, 11(2), 214-224 (2015-01-22)
Both apoptosis ("self-killing") and autophagy ("self-eating") are evolutionarily conserved processes, and their crosstalk influences anticancer drug sensitivity and cell death. However, the underlying mechanism remains unclear. Here, we demonstrated that HMGB1 (high mobility group box 1), normally a nuclear protein
Chih-Hung Lin et al.
Biomedicines, 9(9) (2021-09-29)
Acute kidney injury (AKI) is caused by hypoxia-reoxygenation (H/R), which is a kidney injury produced by a variety of causes, resulting in the remaining portion of the kidney function being unable to maintain the balance for performing the tasks of
Claudio Vinegoni et al.
Nature protocols, 12(7), 1472-1497 (2017-07-08)
The ability to directly image and quantify drug-target engagement and drug distribution with subcellular resolution in live cells and whole organisms is a prerequisite to establishing accurate models of the kinetics and dynamics of drug action. Such methods would thus
Emily L Clark et al.
PLoS genetics, 13(9), e1006997-e1006997 (2017-09-16)
Sheep are a key source of meat, milk and fibre for the global livestock sector, and an important biomedical model. Global analysis of gene expression across multiple tissues has aided genome annotation and supported functional annotation of mammalian genes. We
Ying Pan et al.
Biosensors & bioelectronics, 26(2), 649-654 (2010-07-30)
Urine is the most abundant and easily accessible of all body fluids and provides an ideal route for non-invasive diagnosis of human diseases, particularly of the urinary tract. Electrochemical biosensors are well suited for urinary diagnostics due to their excellent
Chong-Chan Kim et al.
ACS nano, 14(9), 11743-11752 (2020-09-01)
A number of implantable biomedical devices have been developed, and wireless power transfer (WPT) systems are emerging as a way to provide power to these devices without requiring a hardwired connection. Most of the WPT has been based on conventional
Weikai Zhang et al.
International journal of nanomedicine, 6, 535-546 (2011-04-07)
Gene therapy is a potentially effective therapeutic modality for treating sensorineural hearing loss. Nonviral gene delivery vectors are expected to become extremely safe and convenient, and nanoparticles are the most promising types of vectors. However, infrequent nuclear localization in the
Aaron M New et al.
PLoS biology, 12(1), e1001764-e1001764 (2014-01-24)
Organisms respond to environmental changes by adapting the expression of key genes. However, such transcriptional reprogramming requires time and energy, and may also leave the organism ill-adapted when the original environment returns. Here, we study the dynamics of transcriptional reprogramming
Anna Kozłowska et al.
Psychopharmacology, 236(10), 2937-2958 (2019-02-10)
Attention-deficit/hyperactivity disorder (ADHD) is one of the most common neurobehavioural disorders with morphological and functional brain abnormalities. However, there is a growing body of evidence that abnormalities in the immune and endocrine systems may also account for the ADHD pathogenesis.
E Maria Donner et al.
Mutagenesis, 25(1), 49-55 (2009-11-04)
Groups of male B6C3F1 mice were exposed by inhalation to 0, 25, 50, 100 or 200 p.p.m. ethylene oxide (EO) for up to 48 weeks (6 hours/day, 5 days/week). Animals were sacrificed at 6, 12, 24 and 48 weeks after
Dafne Bongiorno et al.
MicrobiologyOpen, 10(2), e1178-e1178 (2021-05-11)
The ability of S. aureus to infect bone and osteoblasts is correlated with its incredible virulence armamentarium that can mediate the invasion/internalization process, cytotoxicity, membrane damage, and intracellular persistence. We comparatively analyzed the interaction, persistence, and modulation of expression of
Lina María Martínez-López et al.
PloS one, 16(8), e0255012-e0255012 (2021-08-20)
Chronic enteropathies are a common problem in dogs, but many aspects of the pathogenesis remain unknown, making the therapeutic approach challenging in some cases. Environmental factors are intimately related to the development and perpetuation of gastrointestinal disease and the gut
Chunyan Wang et al.
Nature communications, 7, 10319-10319 (2016-01-20)
DNA hybridization thermodynamics is critical for accurate design of oligonucleotides for biotechnology and nanotechnology applications, but parameters currently in use are inaccurately extrapolated based on limited quantitative understanding of thermal behaviours. Here, we present a method to measure the ΔG°
Seetharam Deepa et al.
Theriogenology, 161, 161-175 (2020-12-18)
Wnt signaling is conserved among all species and plays a significant role in various cellular processes including reproduction. The present study identified significant involvement of wnt4a, wnt5b, and wnt8a signaling in the testicular growth of common carp,Cyprinus carpio. Predominant expression
Konrad Franke et al.
Translational oncology, 2(4), 310-320 (2009-12-04)
Nodal spread is the single most important prognostic factor of survival in gastric cancer patients. In this study, genes that were upregulated in the lymph node metastases of gastric cancer were identified and may serve as putative novel therapeutic target.
Maturation, Fertilization and Implantation of Cattle Ova Cultured in Different Media
Saif AH, et al.
The eurasia proceedings of science, technology, engineering & mathematics., 3(9), 181-188 (2018)
Alan J Stone et al.
Magnetic resonance in medicine, 86(5), 2512-2527 (2021-07-17)
To characterize microstructural contributions to the magnetic susceptibility of carotid arteries. Arterial vessels were scanned using high-resolution quantitative susceptibility mapping (QSM) at 7 Tesla. Models of vessel degradation were generated using ex vivo porcine carotid arteries that were subjected to
Edit Hrubi et al.
Human cell, 31(2), 139-148 (2018-02-15)
Bone morphogenetic protein-2 (BMP-2), is a potential factor to enhance osseointegration of dental implants. However, the appropriate cellular system to investigate the osteogenic effect of BMP-2 in vitro in a standardized manner still needs to be defined. The aim of
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