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Ralf Küppers et al.
Methods in molecular biology (Clifton, N.J.), 971, 49-63 (2013-01-09)
Normal and malignant B cells carry rearranged immunoglobulin (Ig) variable region genes, which due to their practically limitless diversity represent ideal clonal markers for these cells. We describe here an approach to isolate single cells from frozen tissue sections by
Chao Wang et al.
Journal of medicinal chemistry, 56(6), 2527-2539 (2013-03-06)
The small molecule fusion inhibitors N-(4-carboxy-3-hydroxyphenyl)-2,5-dimethylpyrrole (NB-2) and N-(3-carboxy-4-hydroxyphenyl)-2,5-dimethylpyrrole (A12) target a hydrophobic pocket of HIV-1 gp41 and have moderate anti-HIV-1 activity. In this paper, we report the design, synthesis, and structure-activity relationship of a group of hybrid molecules in
Eva Dvorakova et al.
BMC neurology, 13, 126-126 (2013-09-26)
The accumulation of the misfolded forms of cellular prion protein, i.e. prions (PrPSc), in the brain is one of the crucial characteristics of fatal neurodegenerative disorders, called transmissible spongiform encephalopathies (TSEs). Cellular prion protein is normally linked to the cell
Jessica Varela Villarreal et al.
Journal of microbiological methods, 94(3), 161-169 (2013-07-03)
Molecular techniques, such as polymerase chain reaction (PCR) and quantitative PCR (qPCR), are very sensitive, but may detect total DNA present in a sample, including extracellular DNA (eDNA) and DNA coming from live and dead cells. DNase I is an
Ester Vázquez-Fernández et al.
PloS one, 7(11), e50111-e50111 (2012-11-28)
Elucidation of the structure of PrP(Sc) continues to be one major challenge in prion research. The mechanism of propagation of these infectious agents will not be understood until their structure is solved. Given that high resolution techniques such as NMR
Enzymatic sculpting of nanoscale and microscale surface topographies.
Jen-Huang Huang et al.
Angewandte Chemie (International ed. in English), 51(38), 9619-9623 (2012-08-29)
Young S Oh et al.
American journal of physiology. Cell physiology, 289(3), C576-C581 (2005-04-22)
The polytopic membrane protein presenilin 1 (PS1) is a component of the gamma-secretase complex that is responsible for the intramembranous cleavage of several type I transmembrane proteins, including the beta-amyloid precursor protein (APP). Mutations of PS1, apparently leading to aberrant
Gerhild Zauner et al.
Methods in molecular biology (Clifton, N.J.), 951, 113-127 (2013-01-09)
Liquid chromatography-tandem stage mass spectrometry of glycopeptides is a powerful tool for the site-specific glycosylation analysis of glycoproteins. Using fetuin as a model substance, we describe a protocol for glycopeptide dissection using nonspecific proteolysis by proteinase K. Proteolysis is achieved
Todd W Osmundson et al.
Molecular ecology resources, 13(1), 66-74 (2012-11-06)
The ubiquity, high diversity and often-cryptic manifestations of fungi and oomycetes frequently necessitate molecular tools for detecting and identifying them in the environment. In applications including DNA barcoding, pathogen detection from plant samples, and genotyping for population genetics and epidemiology
Andrea J Wolf et al.
Journal of leukocyte biology, 109(1), 161-172 (2020-09-18)
Malassezia spp. are common eukaryotic yeasts that colonize mammalian skin. Recently, the authors and others have observed that Malassezia globosa and Malassezia restricta can be found in the intestines in the context of certain diseases, including Crohn's disease and pancreatic
Gerhild Zauner et al.
Journal of proteome research, 11(12), 5804-5814 (2012-10-12)
Human fibrinogen is a 340 kDa, soluble plasma glycoprotein composed of paired sets of three subunits (α, β, γ). The protein plays a crucial role in protecting the vascular network against the loss of blood after tissue injury. The beta
Amino acid sequence of proteinase K from the mold Tritirachium album Limber
Jany KD, et al.
Febs Letters, 199(2), 139-144 (2001)
Denaturant-Dependent Conformational Changes in a beta-Trefoil Protein: Global and Residue-Specific Aspects of an Equilibrium Denaturation Process
Latypov RF, et al.
Biochemistry, 48(46), 10934-10947 (2009)
Karen Dobie et al.
Journal of virology, 87(10), 5895-5903 (2013-03-15)
Most current diagnostic tests for transmissible spongiform encephalopathies (TSE) rely on the presence of proteinase K (PK)-resistant PrP(Sc) (PrP-res) in postmortem tissues as an indication of TSE disease. However, a number of studies have highlighted a discrepancy between TSE infectivity
Micaela Pivato et al.
PloS one, 7(12), e50027-e50027 (2012-12-29)
The aggregation of α-synuclein into amyloid fibrils constitutes a key step in the onset of Parkinson's disease. Amyloid fibrils of α-synuclein are the major component of Lewy bodies, histological hallmarks of the disease. Little is known about the mechanism of
Xinping Lin et al.
Yeast (Chichester, England), 29(11), 467-474 (2012-10-16)
Red yeasts hold great promise in the production of microbial lipids and carotenoids. Genetic study of red yeasts has attracted much attention; however, rapid amplification of genes from red yeast samples remains technically challenging. Here a highly efficient method for
Yoshitaka Yamaguchi et al.
PloS one, 7(8), e43540-e43540 (2012-08-29)
Accumulating lines of evidence indicate that the N-terminal domain of prion protein (PrP) is involved in prion susceptibility in mice. In this study, to investigate the role of the octapeptide repeat (OR) region alone in the N-terminal domain for the
Neetu Kumra Taneja et al.
Journal of bacteriology, 195(22), 5102-5111 (2013-09-10)
Bordetella pertussis is the causative agent of pertussis, a highly contagious disease of the human respiratory tract. Despite very high vaccine coverage, pertussis has reemerged as a serious threat in the United States and many developing countries. Thus, it is
Han Sun et al.
Bing du xue bao = Chinese journal of virology, 28(4), 409-413 (2012-09-18)
In order to explore the potential influences of the disulfide bridge on the physical and chemical properties of PrP protein, the expressed recombinant human wild-type PrP protein was purified for using in an established redox process for the reduction and
Sudhir Kumar Shukla et al.
The Journal of antibiotics, 66(2), 55-60 (2012-11-15)
The dominant role of biofilm-associated protein (Bap) in Staphylococcus aureus biofilm development prompted us to investigate Bap as a potential target for proteinase-mediated biofilm dispersion. Biofilm assay in microtitre plates showed that proteinase K hampered the early adhesion of cells
Yi-sheng Chen et al.
Archives of microbiology, 196(3), 193-199 (2014-02-05)
Lactobacillus plantarum 510, previously isolated from a koshu vineyard in Japan, was found to produce a bacteriocin-like inhibitory substance which was purified and characterized. Mass spectrometry analysis showed that the mass of this bacteriocin is 4,296.65 Da. A partial sequence, NH2-
Amudha Nagarathinam et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 33(49), 19284-19294 (2013-12-07)
Pathological, genetic, and biochemical hallmarks of Alzheimer's disease (AD) are linked to amyloid-β (Aβ) peptide aggregation. Especially misfolded Aβ42 peptide is sufficient to promote amyloid plaque formation. However, the cellular compartment facilitating the conversion of monomeric Aβ to aggregated toxic
Fangzhong Yuan et al.
Journal of molecular neuroscience : MN, 51(1), 219-224 (2013-05-30)
Prion diseases characterize a category of fatal neurodegenerative diseases. Although reports have increasingly shown that oxidative stress plays an important role in the progression of prion diseases, little is known about whether oxidative stress is a cause or a consequence
The specificity of proteinase K against oxidized insulin B chain.
E Kraus et al.
Hoppe-Seyler's Zeitschrift fur physiologische Chemie, 357(2), 233-237 (1976-02-01)
Karine Berthelot et al.
PloS one, 7(10), e48065-e48065 (2012-11-08)
REF (Hevb1) and SRPP (Hevb3) are two major components of Hevea brasiliensis latex, well known for their allergenic properties. They are obviously taking part in the biosynthesis of natural rubber, but their exact function is still unclear. They could be
Protein-protected Au clusters as a new class of nanoscale biosensor for label-free fluorescence detection of proteases.
Yucai Wang et al.
Small (Weinheim an der Bergstrasse, Germany), 8(24), 3769-3773 (2012-09-13)
Proteinase K from Tritirachium album Limber.
W Ebeling et al.
European journal of biochemistry, 47(1), 91-97 (1974-08-15)
Martin L Daus et al.
The Journal of biological chemistry, 288(49), 35068-35080 (2013-10-29)
The self-replicative conformation of misfolded prion proteins (PrP) is considered a major determinant for the seeding activity, infectiousness, and strain characteristics of prions in different host species. Prion-associated seeding activity, which converts cellular prion protein (PrP(C)) into Proteinase K-resistant, infectious
Jian Liu et al.
Applied and environmental microbiology, 79(18), 5593-5600 (2013-07-10)
Stenotrophomonas maltophilia is an important global opportunistic pathogen for which limited therapeutics are available because of the emergence of multidrug-resistant strains. A novel bacteriocin, maltocin P28, which is produced by S. maltophilia strain P28, may be the first identified phage
Mary H Young et al.
PloS one, 8(1), e53789-e53789 (2013-01-24)
Mucosal-associated invariant T cells are a unique population of T cells that express a semi-invariant αβ TCR and are restricted by the MHC class I-related molecule MR1. MAIT cells recognize uncharacterized ligand(s) presented by MR1 through the cognate interaction between
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