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Purification of beta-lactamases on QAE-sephadex.
G W Ross et al.
Biochimica et biophysica acta, 309(2), 430-439 (1973-06-06)
L Metwally et al.
Eastern Mediterranean health journal = La revue de sante de la Mediterranee orientale = al-Majallah al-sihhiyah li-sharq al-mutawassit, 19(11), 947-952 (2014-03-29)
The emergence and rapid spread of antibiotic-resistant Klebsiella pneumoniae isolates harbouring the blaKPC gene that encodes for carbapenemase production have complicated the management of patient infections. This study in a tertiary care hospital in Egypt used real-time PCR assay to
Teerawit Tangkoskul et al.
The Southeast Asian journal of tropical medicine and public health, 43(6), 1461-1469 (2013-02-19)
We performed extended-spectrum beta-lactamase (ESBL) phenotypic testing and molecular characterization of three ESBL genes (TEM, SHV and CTX-M) and susceptibility testing by Clinical Laboratory Standards Institute (CLSI) disk diffusion method against three cephalosporins (ceftriaxone, ceftazidime, cefepime) and a cephamycin (cefoxitin)
Despina Kotsanas et al.
The Medical journal of Australia, 198(5), 267-269 (2013-03-19)
Clinical utility of carbapenem antibiotics is under threat because of the emergence of acquired metallo-β-lactamase (MBL) genes. We describe an outbreak in an intensive care unit (ICU) possibly associated with contaminated sinks. Four clusters of gram-negative bacteria harbouring the MBL
V S M Saegeman et al.
Acta clinica Belgica, 69(1), 30-32 (2014-03-19)
Prevalence of carbapenemase-producing Enterobacteriaceae (CPE) is increasing both in hospitals and in the community. In this point prevalence study, rectal CPE colonization was investigated among 150 nursing home residents. No CPE were detected. Epidemiological data directly linked with CPE colonization
Grace Yim et al.
Canadian journal of microbiology, 59(2), 110-116 (2013-03-07)
Microbial populations in wastewater treatment plants (WWTPs) are increasingly being recognized as environmental reservoirs of antibiotic resistance genes. PCR amplicons for plasmid-mediated quinolone resistance determinants qnrA, qnrB, and qnrS were recorded in samples from a WWTP in Vancouver, British Columbia.
H Pîrvănescu et al.
Chirurgia (Bucharest, Romania : 1990), 109(1), 73-79 (2014-02-15)
Wound infections remain a public health problem, despite the progress made on improving surgical techniques and antibiotic prophylaxis application. Misuse of antibiotics to prevent bacterial infections leads to increased bacterial resistance and their dissemination. The study refers to 470 samples
Amit Kumar et al.
Pakistan journal of biological sciences : PJBS, 17(1), 109-113 (2014-05-03)
E. coli, often highly pathogenic in neonatal and immuno-compromised patients, are usually supposed susceptible to a variety of chemotherapeutic agents, however with the time and also due to the extensive use of chemotherapeutic agents irrespective of their susceptibility, have evolved
Chanapai Chaiyakulsil et al.
Journal of the Medical Association of Thailand = Chotmaihet thangphaet, 95 Suppl 12, S138-S141 (2013-03-22)
Extended-spectrum beta-lactamase (ESBL) producing organisms cause wide spectrum of diseases including urinary tract infection, cholangitis, intra-abdominal abscess or pneumonia but rarely meningitis. The present report a successful nonsurgical, medical treatment in a child with Escherichia coli ESBL meningitis with acute
Lena P Setchanova et al.
Annals of clinical microbiology and antimicrobials, 12, 6-6 (2013-03-28)
Pneumococcal and Haemophilus influenzae type b (Hib) vaccines were introduced in our national immunisation program in April 2010. The aims of this retrospective, laboratory-based study were to determine the serotypes and antibiotic resistance of Streptococcus pneumoniae and H. influenzae isolates
Siriluck Anunnatsiri et al.
The Southeast Asian journal of tropical medicine and public health, 43(5), 1169-1177 (2013-02-26)
Escherichia coli producing extended spectrum beta-lactamase (ESBL) has emerged as a worldwide, public health problem. The aims of this study were to determine the incidence of ESBL-producing E. coli septicemia and evaluate the factors associated with the infection and the
Jun Hirose et al.
The Journal of experimental medicine, 211(1), 153-163 (2013-12-25)
Signal transducer and activator of transcription 5 (Stat5) is essential for cytokine-regulated processes such as proliferation, differentiation, and survival in hematopoietic cells. To investigate the role of Stat5 in osteoclasts, we generated mice with an osteoclast-specific conditional deletion of Stat5
Refath Farzana et al.
The Southeast Asian journal of tropical medicine and public health, 44(1), 96-103 (2013-05-21)
From a total of 320 bacterial samples from wound swab and urine 169 (53%) gram-negative bacteria were isolated, of which 42 (25%) extended-spectrum beta-lactamase (ESBL) producers were detected by double-disk synergy test. ESBL producers were significantly more resistant against amoxiclav
Paolo Gaibani et al.
The new microbiologica, 37(1), 87-90 (2014-02-18)
We describe an outbreak of a carbapenemase-producing Klebsiella pneumoniae sequence type 258 (ST258) clone in an Italian hospital during two months in 2010. The rapid detection and management of the eleven patients colonized and infected with KPC-producing K.pneumoniae curbed the
Structural biology scales down.
Robert F Service
Science (New York, N.Y.), 343(6175), 1072-1073 (2014-03-08)
Ina Willemsen et al.
Journal of medical microbiology, 63(Pt 4), 540-543 (2014-01-30)
We investigated the performance of a real-time PCR for the detection of extended spectrum β-lactamase genes in Enterobacteriaceae (Check-MDR ESBL PCR). Results from micro-arrays were considered as the gold standard. An analysis on 489 isolates resulted in a sensitivity of
Seung Bok Hong et al.
Journal of medical microbiology, 62(Pt 6), 836-844 (2013-03-23)
The co-existence of carbapenemase, 16S rRNA methylase and mutated quinolone resistance-determining regions (QRDRs) can cause serious difficulty in treating infections with multidrug-resistant Acinetobacter baumannii. In this study, we aimed to determine the mechanisms of imipenem, amikacin and ciprofloxacin resistance in
Hasan Nazik et al.
The Southeast Asian journal of tropical medicine and public health, 43(5), 1178-1185 (2013-02-26)
Bacterial isolates producing Class D OXA-48 carbapenemase may be missed in routine laboratory testing, allowing them to spread undetected. The purpose of the present study was to detect bla(OXA-48) among ESBL-producing Klebsiella pneumoniae and Escherichia coli isolates collected from a
Characterization of the first isolate of Klebsiella pneumoniae carrying New Delhi metallo-β-lactamase and other extended spectrum β-lactamase genes from Malaysia.
Norazah Ahmad et al.
Journal of medical microbiology, 62(Pt 5), 804-806 (2013-03-02)
Carbapenem-resistant Enterobacteriaceae: what has happened, and what is being done.
Patrick R Murray
MLO: medical laboratory observer, 45(5), 26-26 (2013-06-15)
Samuel Wagner et al.
Veterinary microbiology, 169(3-4), 171-178 (2014-02-04)
Multidrug-resistant Escherichia coli is an emerging clinical challenge in domestic species. Treatment options in many cases are limited. This study characterized MDR E. coli isolates from urinary tract infections in dogs, collected between 2002 and 2011. Isolates were evaluated in
MMWR. Morbidity and mortality weekly report, 62(6), 108-108 (2013-02-15)
On August 16, 2012, the Colorado Department of Public Health and Environment was notified of two patients at an acute-care hospital in Denver with carbapenem-resistant Enterobacteriaceae (CRE), specifically Klebsiella pneumoniae (CRKP), isolated from respiratory specimens during July-August. Both isolates produced
Ian Morrissey et al.
Journal of medical microbiology, 63(Pt 4), 556-561 (2014-01-31)
A subset of Escherichia coli, Klebsiella oxytoca, Klebsiella pneumoniae and Proteus mirabilis isolates collected for the Study for Monitoring Antimicrobial Resistance Trends that were positive for the Clinical and Laboratory Standards Institute (CLSI) extended-spectrum β-lactamase (ESBL) phenotypic confirmatory test (n = 3245)
Dianny Martínez et al.
Revista do Instituto de Medicina Tropical de Sao Paulo, 56(1), 67-69 (2014-02-21)
Clinical strains of Enterobacter were isolated from Cumana's Central Hospital in Venezuela, and classified as E. cloacae (21), E. aerogenes (7), E. intermedium (1), E. sakazakii (1) and three unclassified. The strains showed high levels of resistance, especially to SXT
Josefina Pastrana-Carrasco et al.
Revista de investigacion clinica; organo del Hospital de Enfermedades de la Nutricion, 64(6 Pt 1), 535-540 (2013-03-22)
To determine the frequency of the gene qacEdelta1 and characterize the resistance to biocides of extended-spectrum beta-lactamases producing enterobacteriaceae (ESBL-PE) obtained from clinical isolates causing nosocomial infections. In total 59 ESBL-PE causing nosocomial infections were included: Klebsiella pneumoniae (35) and
T D Hennessey et al.
The Biochemical journal, 109(3), 469-473 (1968-09-01)
1. A beta-lactamase has been purified from a strain of Enterobacter cloacae. 2. This enzyme is about eighty times as active against cephaloridine as against benzylpenicillin or ampicillin. 3. The enzyme has a net positive charge at pH8.0 and a
Jiri Klimes et al.
Journal of zoo and wildlife medicine : official publication of the American Association of Zoo Veterinarians, 44(1), 173-175 (2013-03-20)
Only a few reports exist on the occurrence of resistant bacteria in zoo animals. Therefore, an isolation of multiresistant Escherichia coli from the lungs of a captive South American tapir (Tapirus terrestris) lead to its characterization and further investigation of
Joseph E Rubin et al.
Veterinary microbiology, 170(1-2), 10-18 (2014-03-01)
Organisms in the family Enterobactericeae including, Escherichia coli, commonly cause community and hospital-associated infections in both humans and companion animals. The increasing prevalence of infections with organisms producing broad spectrum β-lactamses such as the ESBLs (particularly the CTX-M type), AmpC
Mahboube Bagheri et al.
International journal of food microbiology, 177, 16-20 (2014-03-05)
Two hundred and four Escherichia coli strains were isolated from external and visceral cavity surfaces of 102 slaughtered broiler carcasses. The isolates were screened to determine the phylogenetic background and presence of Shiga toxins (stx1, stx2), intimin (eae) and beta-lactamase
Mayuri Trivedi et al.
The Journal of the Association of Physicians of India, 60, 28-30 (2013-02-15)
In India where the prevalence of extended spectrum beta lactamase (ESBL) producing organisms among gram negative organisms is 60-70% and Ertapenem was unavailable at the beginning of this study, exclusive use of Group 2 Carbapenems (Imipenem and Meropenem) for treatment
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