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Thomas R L Klei et al.
Blood advances, 4(24), 6218-6229 (2020-12-23)
Senescence of erythrocytes is characterized by a series of changes that precede their removal from the circulation, including loss of red cell hydration, membrane shedding, loss of deformability, phosphatidyl serine exposure, reduced membrane sialic acid content, and adhesion molecule activation.
Mikail Temirel et al.
Micromachines, 12(2) (2021-03-07)
Hemp is a sustainable, recyclable, and high-yield annual crop that can be used to produce textiles, plastics, composites, concrete, fibers, biofuels, bionutrients, and paper. The integration of microfluidic paper-based analytical devices (µPADs) with hemp paper can improve the environmental friendliness
Dongxu He et al.
EMBO molecular medicine, 9(11), 1491-1503 (2017-09-14)
The currently available antihypertensive agents have undesirable adverse effects due to systemically altering target activity including receptors, channels, and enzymes. These effects, such as loss of potassium ions induced by diuretics, bronchospasm by beta-blockers, constipation by Ca
Pabitra B Pal et al.
Endocrinology, 158(10), 3661-3675 (2017-09-25)
Despite recent studies that show oxidative stress-generated reactive oxygen species (ROS) regulate NOD-like receptor family pyrin domain containing 3 (NLRP3) inflammasome-mediated innate immune response in various diabetic complications, the mechanism by which ROS activate innate immune response is not well
K Venema et al.
Biochimica et biophysica acta, 1146(1), 87-96 (1993-02-23)
Liposomes of egg PC/PG (8:2, mol/mol) were multilabelled with PBFI, pyranine and oxonol VI, fluorescent probes for, respectively, K+, H+ and membrane potential. Monitoring fluorescence with a multichannel photoncounting spectrofluorometer during K+ filling experiments allowed to measure K+ influx, the
X Chen et al.
Biochemistry, 33(46), 13769-13774 (1994-11-22)
Although the predominance of plasmalogens in electrically-active membranes (e.g., sarcolemma) is well-known, identification of the molecular mechanisms through which the vinyl ether linkage facilitates electrophysiologic function has remained elusive. Herein we demonstrate that the kinetics of both carrier-mediated (i.e., valinomycin)
Lars F Olsen et al.
Archives of biochemistry and biophysics, 681, 108257-108257 (2020-01-10)
We measured temporal oscillations of intracellular K+ concentration in yeast cells exhibiting glycolytic oscillations using fluorescence spectroscopy and microscopy methods. These oscillations showed the same period as those of glycolytic metabolites (NADH, ATP), indicating a strong coupling between them. We
Tzu-Hsuan Chang et al.
PLoS pathogens, 13(7), e1006485-e1006485 (2017-07-04)
Inflammasome is an intracellular protein complex that serves as cytosolic pattern recognition receptor (PRR) to engage with pathogens and to process cytokines of the interleukin-1 (IL-1) family into bioactive molecules. It has been established that interleukin-1β (IL-1β) is important to
Daniel Soetkamp et al.
Basic research in cardiology, 109(5), 433-433 (2014-08-15)
S-nitrosation (SNO) of connexin 43 (Cx43)-formed channels modifies dye uptake in astrocytes and gap junctional communication in endothelial cells. Apart from forming channels at the plasma membrane of several cell types, Cx43 is also located at the inner membrane of
P Jezek et al.
The Journal of biological chemistry, 265(18), 10522-10526 (1990-06-25)
New indicators for fluorescent measurement of Na+ and K+ ions should prove particularly useful for studies of reconstituted carriers of these ions. We show that PBFI, a K(+)-specific probe, provides a convenient and sensitive assay for the study of K+
Ahmed G Abdelhamid et al.
Applied and environmental microbiology, 85(14) (2019-05-19)
Salmonella enterica is increasingly linked to disease outbreaks associated with consumption of low-water-activity (low-aw) foods. Persistence of the pathogen in these foods was attributed to its ability to implement desiccation resistance mechanisms. Published knowledge about methods that disrupt desiccation resistance
MeiYing Yang et al.
Biochimica et biophysica acta, 1858(6), 442-458 (2017-03-28)
We provide evidence for location and function of a small conductance, Ca
Mehdi Rahimi et al.
Luminescence : the journal of biological and chemical luminescence, 34(4), 402-406 (2019-04-30)
Photophysical phenomena associated with carbon nanoparticles in combination with biocompatibility and readily functionalizable properties have attracted significant interest for sensing and imaging applications. A potassium ion optode based on the fluorescence quenching of carbon quantum dots (CQDs) was constructed. The
Kerstin Boengler et al.
British journal of pharmacology, 174(13), 2060-2073 (2017-04-04)
Dysregulation of gap junction-mediated cell coupling contributes to development of arrhythmias and myocardial damage after ischaemia/reperfusion (I/R). Connexin 43 (Cx43) is present at ventricular gap junctions and also in the mitochondria of cardiomyocytes. The dipeptide (2S, 4R)-1-(2-aminoacetyl)-4-benzamidopyrrolidine-2-carboxylic acid (ZP1609) has
Yanying Liu et al.
Journal of Alzheimer's disease : JAD, 73(3), 919-933 (2019-12-31)
Alzheimer's disease (AD) is a devastating neurodegenerative disorder, neuropathologically characterized by hyperphosphorylation of tau and formation of amyloid plaques. Most AD cases are sporadic with no clear cause. Cell models play an important role in understanding the pathogenesis of sporadic
Xianfeng Zhou et al.
Life (Basel, Switzerland), 10(4) (2020-04-17)
Two important ions, K+ and Na+, are unequally distributed across the contemporary phospholipid-based cell membrane because modern cells evolved a series of sophisticated protein channels and pumps to maintain ion gradients. The earliest life-like entities or protocells did not possess
Shu-Hong Zhang et al.
The Journal of biological chemistry, 294(16), 6375-6386 (2019-02-23)
Contactin-associated protein 1 (CASPR1 or CNTNAP1) was recently reported to be expressed in brain microvascular endothelial cells (BMECs), the major component of the blood-brain barrier. To investigate CASPR1's physiological role in BMECs, here we used CASPR1 as a bait in
P Paucek et al.
The Journal of biological chemistry, 267(36), 26062-26069 (1992-12-25)
The transport properties of mitochondria are such that net potassium flux across the inner membrane determines mitochondrial volume. It has been known that K+ uptake is mediated by diffusive leak driven by the high electrical membrane potential maintained by redox-driven
Sun Hee Moon et al.
BMC microbiology, 19(1), 6-6 (2019-01-10)
There is an urgent need to develop potent antimicrobials for the treatment of infections caused by antibiotic-resistant bacterial pathogens. Paenipeptin C' (C8-Pat) is a novel linear lipopeptide recently discovered by our group. The objectives of this study were to determine
Chelsea Marie et al.
Scientific reports, 5, 13613-13613 (2015-09-09)
The parasite Entamoeba histolytica kills human cells resulting in ulceration, inflammation and invasion of the colonic epithelium. We used the cytotoxic properties of ameba to select a genome-wide RNAi library to reveal novel host factors that control susceptibility to amebic
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