Showing 1-30 of 173 results for "P6757"
Brian T Nasipak et al.
Nature communications, 6, 7441-7441 (2015-06-18)
Calcium signalling is important for differentiation-dependent gene expression, but is also involved in other cellular functions. Therefore, mechanisms must exist to distinguish calcium signalling relevant to differentiation. Calcineurin is a calcium-regulated phosphatase that is required for myogenic gene expression and...
Seneha Santoshi et al.
Journal of computer-aided molecular design, 29(3), 249-270 (2014-12-08)
We have strategically designed a series of noscapine derivatives by inserting biaryl pharmacophore (a major structural constituent of many of the microtubule-targeting natural anticancer compounds) onto the scaffold structure of noscapine. Molecular interaction of these derivatives with α,β-tubulin heterodimer was...
Magnus Monné et al.
Biochimica et biophysica acta, 1847(10), 1220-1230 (2015-07-05)
The Arabidopsis thaliana genome contains 58 membrane proteins belonging to the mitochondrial carrier family. Three members of this family, here named AtAPC1, AtAPC2, and AtAPC3, exhibit high structural similarities to the human mitochondrial ATP-Mg(2+)/phosphate carriers. Under normal physiological conditions the...
Richard Lu et al.
PLoS pathogens, 11(10), e1005200-e1005200 (2015-10-17)
The intracellular bacterial pathogen Shigella infects and spreads through the human intestinal epithelium. Effector proteins delivered by Shigella into cells promote infection by modulating diverse host functions. We demonstrate that the effector protein OspB interacts directly with the scaffolding protein...
Rangarajan Padmanabhan et al.
Journal of cell science, 128(9), 1773-1786 (2015-03-22)
The epithelial-to-mesenchymal transition (EMT) is important for the formation of migratory neural crest cells during development and is co-opted in human diseases such as cancer metastasis. Chick premigratory cranial neural crest cells lose intercellular contacts, mediated in part by Cadherin-6B...
Takayuki Sekimoto et al.
Molecular and cellular biology, 35(4), 699-715 (2014-12-10)
DNA rereplication is a major form of aberrant replication that causes genomic instabilities, such as gene amplification. However, little is known about which DNA polymerases are involved in the process. Here, we report that low-fidelity Y-family polymerases (Y-Pols), Pol η...
Aurélien Chollet et al.
Journal of structural biology, 190(3), 328-337 (2015-04-22)
InhA is an enoyl-ACP reductase of Mycobacterium tuberculosis implicated in the biosynthesis of mycolic acids, essential constituents of the mycobacterial cell wall. To date, this enzyme is considered as a promising target for the discovery of novel antitubercular drugs. In...
Ranran Wang et al.
Cancer research, 74(12), 3332-3343 (2014-04-17)
BRD4 is implicated in the pathogenesis of a number of different cancers. It is also the target of translocation t(15;19) that accounts for the highly aggressive NUT midline carcinoma (NMC). We discovered that t(15;19) NMC cells display the ability to...
K Yamamoto et al.
Histochemistry, 77(3), 339-351 (1983-01-01)
Effects of NaOH-PIPES buffer used as a vehicle for aldehyde fixative on alkaline phosphatase (ALPase) activity demonstrated cyto- and biochemically were compared with those of routinely used cacodylate buffer. The reaction products showing ALPase activity demonstrated ultracytochemically were confined to...
J Hatae et al.
Biological & pharmaceutical bulletin, 17(3), 437-439 (1994-03-01)
1,4-Piperazinediethanesulfonic acid (PIPES), a zwitterion buffer, was titrated with KOH to measure its pH and osmolality. Both pH and osmolality changes showed three phases, and two transition points coincided with pH 5.1 and 10.2. The second phase is important in...
Qi Miao et al.
Nature communications, 5, 4088-4088 (2014-06-10)
Cell migration is an integral part of re-epithelialization during skin wound healing, a complex process involving molecular controls that are still largely unknown. Here we identify a novel role for Tcf3, an essential transcription factor regulating embryonic and adult skin...
R L Schultz et al.
Journal of neurocytology, 15(4), 461-469 (1986-08-01)
When used for vascular perfusion of brain, 0.1 M PIPES-buffered 3% glutaraldehyde resulted in the formation of expanded, vesicle-filled cell processes limited by multiple membrane layers. These structures, termed multivesicular myelin figures and interpreted as artefacts, were most common in...
V D Prokopieva et al.
Alcohol and alcoholism (Oxford, Oxfordshire), 35(1), 44-48 (2000-02-24)
The effects of carnosine and related compounds on erythrocytes from alcoholics were studied. In their presence, erythrocytes showed an increased ability to resist haemolysis and showed a more normal morphology, with carnosine and N-acetyl-carnosine being the most effective compounds. These...
Peter J Chung et al.
Proceedings of the National Academy of Sciences of the United States of America, 112(47), E6416-E6425 (2015-11-07)
Microtubules (MTs) are hollow cytoskeletal filaments assembled from αβ-tubulin heterodimers. Tau, an unstructured protein found in neuronal axons, binds to MTs and regulates their dynamics. Aberrant Tau behavior is associated with neurodegenerative dementias, including Alzheimer's. Here, we report on a...
Shuo Jiang et al.
Developmental cell, 49(5), 711-730 (2019-04-30)
The correct localization of Hedgehog effectors to the tip of primary cilia is critical for proper signal transduction. The conserved non-motile kinesin Kif7 defines a "cilium-tip compartment" by localizing to the distal ends of axonemal microtubules. How Kif7 recognizes microtubule ends...
V Kaushal et al.
Cell death and differentiation, 22(10), 1676-1686 (2015-03-07)
Neuronal active Caspase-6 (Casp6) is associated with Alzheimer disease (AD), cognitive impairment, and axonal degeneration. Caspase-1 (Casp1) can activate Casp6 but the expression and functionality of Casp1-activating inflammasomes has not been well-defined in human neurons. Here, we show that primary...
Derrick L Lewis et al.
Extremophiles : life under extreme conditions, 19(2), 269-281 (2014-12-05)
A mutant ('lab strain') of the hyperthermophilic archaeon Pyrococcus furiosus DSM3638 exhibited an extended exponential phase and atypical cell aggregation behavior. Genomic DNA from the mutant culture was sequenced and compared to wild-type (WT) DSM3638, revealing 145 genes with one...
G Pethö
Acta physiologica Hungarica, 83(2), 113-119 (1995-01-01)
Compounds with extended conjugated electron-system are under constant study in view of their numerous biological functions. Among them natural and synthetic porphyrins are of particular interest because of their activity in photodynamic treatment of cancer and virus infection. Recently, many...
Olivera Magdenoska et al.
Analytical biochemistry, 487, 17-26 (2015-07-05)
For quantification of intracellular metabolites, mass spectrometry combined with liquid chromatography, capillary electrophoresis, or gas chromatography is currently the method of choice, especially when combined with stable isotope-labeled internal standards (SIL-ISs). Due to the difficulties in finding a biological matrix...
Ivo A Hendriks et al.
Nature communications, 6, 7289-7289 (2015-06-16)
SUMOylation is a reversible post-translational modification (PTM) regulating all nuclear processes. Identification of SUMOylation sites by mass spectrometry (MS) has been hampered by bulky tryptic fragments, which thus far necessitated the use of mutated SUMO. Here we present a SUMO-specific...
G W Mainwood et al.
Advances in experimental medicine and biology, 248, 611-619 (1989-01-01)
In this study we measured changes in intracellular ATP and pH together with lactate production in isolated ischemic human atrial tissue. The measurements were made using 31P and 1H NMR. ATP preservation is improved as temperature is reduced from 20...
Haejin Lee et al.
PloS one, 9(8), e104092-e104092 (2014-08-12)
Cell transplantation has been suggested as an alternative therapy for temporal lobe epilepsy (TLE) because this can suppress spontaneous recurrent seizures in animal models. To evaluate the therapeutic potential of human neural stem/progenitor cells (huNSPCs) for treating TLE, we transplanted...
Mariano Bisbal et al.
Journal of neurochemistry, 146(5), 570-584 (2018-07-05)
Rotenone, a broad-spectrum insecticide, piscicide and pesticide, produces a complete and selective suppression of axonogenesis in cultured hippocampal neurons. This effect is associated with an inhibition of actin dynamics through activation of Ras homology member A (RhoA) activity. However, the...
Lei Yu et al.
Cancer & metabolism, 7, 13-13 (2020-01-02)
Pancreatic ductal adenocarcinoma (PDAC) is an aggressive cancer with limited treatment options. Pyruvate kinase, especially the M2 isoform (PKM2), is highly expressed in PDAC cells, but its role in pancreatic cancer remains controversial. To investigate the role of pyruvate kinase...
Marleen M Janus et al.
Biofouling, 31(6), 503-510 (2015-07-28)
Commensal oral biofilms, defined by the absence of pathology-related phenotypes, are ubiquitously present. In contrast to pathological biofilms commensal biofilms are rarely studied. Here, the effect of the initial inoculum and subsequent growth conditions on in vitro oral biofilms was...
Jorge Oliver-De La Cruz et al.
PloS one, 9(6), e99253-e99253 (2014-06-06)
A number of neurodegenerative diseases progress with a loss of myelin, which makes them candidate diseases for the development of cell-replacement therapies based on mobilisation or isolation of the endogenous neural/glial progenitor cells, in vitro expansion, and further implantation. Cells...
Sergey V Rudenko
Blood cells, molecules & diseases, 42(3), 252-261 (2009-03-03)
The dynamics of red cell shape changes following transfer into sucrose media having a low chloride content was studied. Based on a large number of measurements, six types of morphological response (MR), differing both in the degree of shape changes...
Puneet Singh et al.
Obesity (Silver Spring, Md.), 23(11), 2286-2293 (2015-09-27)
To identify glucocorticoid receptor (GR)-associated chromatin sequences and target genes in primary human abdominal subcutaneous fat. GR chromatin immunoprecipitation (ChIP)-sequencing (seq) methodology in subcutaneous human adipocytes treated ex vivo with dexamethasone (dex) was optimized to identify genome-wide dex-dependent GR-binding regions...
A N Koppes et al.
Journal of neural engineering, 11(4), 046002-046002 (2014-06-04)
Both electrical stimuli (endogenous and exogenous) and topographical cues are instructive to axonal extension. This report, for the first time, investigated the relative dominance of directional topographical guidance cues and directional electrical cues to enhance and/or direct primary neurite extension....
Véronique Martel-Frachet et al.
Oncotarget, 6(16), 14669-14686 (2015-06-04)
We previously identified 1-(2,4-dimethoxyphenyl)-3-(1-methylindolyl) propenone (IPP51), a new chalcone derivative that is capable of inducing prometaphase arrest and subsequent apoptosis of bladder cancer cells. Here, we demonstrate that IPP51 selectively inhibits proliferation of tumor-derived cells versus normal non-tumor cells. IPP51...

Social Media

LinkedIn icon
Twitter icon
Facebook Icon
Instagram Icon


Research. Development. Production.

We are a leading supplier to the global Life Science industry with solutions and services for research, biotechnology development and production, and pharmaceutical drug therapy development and production.

© 2021 Merck KGaA, Darmstadt, Germany and/or its affiliates. All Rights Reserved.

Reproduction of any materials from the site is strictly forbidden without permission.