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p7892

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Simona Lobasso et al.
Photochemistry and photobiology, 88(3), 690-700 (2012-01-18)
We have isolated and characterized the light-driven proton pump Bop I from the ultrathin square archaeon Haloquadratum walsbyi, the most abundant component of the dense microbial community inhabiting hypersaline environments. The disruption of cells by hypo-osmotic shock yielded Bop I
O Ploux et al.
The Biochemical journal, 283 ( Pt 2), 327-331 (1992-04-15)
The 8-amino-7-oxopelargonate synthase [6-carboxyhexanoyl-CoA:L-alanine carboxyhexanoyltransferase (decarboxylating); EC 2.3.1.47] from Bacillus sphaericus involved in biotin biosynthesis was purified from an Escherichia coli overproducing strain. The purification afforded an electrophoretically homogeneous enzyme with a specific activity of 0.67 unit/mg. The purified enzyme
Stabilization of <I>Nocardia</I> EH1 epoxide hydrolase by immobilization.
Kroutil, W., et al.
Biotechnology Letters, 20(4), 373-377 (1998)
Cui, W., et al.
Enzyme and Microbial Technology, 24(3-4), 200-208 (1999)
S K Shenoy et al.
The Journal of biological chemistry, 272(28), 17867-17872 (1997-07-11)
The cDNA encoding the smallest membrane-anchoring subunit (QPs3) of bovine heart mitochondrial succinate-ubiquinone reductase was cloned and sequenced. This cDNA is 1330 base pairs long with an open reading frame of 474 base pairs that encodes the 103 amino acid
Han-Seung Joo et al.
Journal of microbiology and biotechnology, 22(1), 58-68 (2012-02-03)
An investigation was conducted on the enhancement of production and purification of an oxidant and SDS-stable alkaline protease (BHAP) secreted by an alkalophilic Bacillus horikoshii, which was screened from the body fluid of a unique Korean polychaeta (Periserrula leucophryna) living
Teresa Martínez-Cortés et al.
Plant physiology and biochemistry : PPB, 52, 130-139 (2012-02-07)
Two cationic peroxidases from Selaginella martensii Spring. (SmaPrx2 and SmaPrx3) were purified using a three-step protocol which includes ammonium sulfate precipitation, adsorption chromatography on phenyl sepharose and cationic exchange chromatography on SP sepharose. The molecular mass for SmaPrx2 and SmaPrx3
W Kroutil et al.
Journal of biotechnology, 61(2), 143-150 (1998-07-09)
A highly enantioselective, soluble epoxide from Nocardia sp. EH1 was purified to homogeneity via a four-step procedure: (i) hydrophobic interaction chromatography on Phenyl Sepharose CL-4B, (ii) anion exchange chromatography on SOURCE 30Q, followed by (iii) a second hydrophobic interaction chromatography
Sheref S Mansy et al.
Nature, 454(7200), 122-125 (2008-06-06)
Contemporary phospholipid-based cell membranes are formidable barriers to the uptake of polar and charged molecules ranging from metal ions to complex nutrients. Modern cells therefore require sophisticated protein channels and pumps to mediate the exchange of molecules with their environment.
Purification and properties of hepatic glutamine synthetases from the ureotelic gulf toadfish, <I>Opsanus beta</I>.
Walsh, P.J., et al.
Comp. Biochem. Physiol., B: Comp. Biochem., 115(4), 523-532 (1996)
Sheref S Mansy et al.
Proceedings of the National Academy of Sciences of the United States of America, 105(36), 13351-13355 (2008-09-05)
The earliest cells may have consisted of a self-replicating genetic polymer encapsulated within a self-replicating membrane vesicle. Here, we show that vesicles composed of simple single-chain amphiphiles such as fatty acids, fatty alcohols, and fatty-acid glycerol esters are extremely thermostable
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