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Andreas Bosshart et al.
Chembiochem : a European journal of chemical biology, 16(4), 592-601 (2015-02-07)
Functional promiscuity of enzymes can often be harnessed as the starting point for the directed evolution of novel biocatalysts. Here we describe the divergent morphing of an engineered thermostable variant (Var8) of a promiscuous D-tagatose epimerase (DTE) into two efficient
Anna Lluveras-Tenorio et al.
Chemistry Central journal, 6(1), 115-115 (2012-10-12)
Saccharide materials have been used for centuries as binding media, to paint, write and illuminate manuscripts and to apply metallic leaf decorations. Although the technical literature often reports on the use of plant gums as binders, actually several other saccharide
Akram Hossain et al.
Biochemical and biophysical research communications, 425(4), 717-723 (2012-08-11)
Rare sugar D-psicose has cropped up as a non-toxic and effective compound to protect and preserve pancreatic β-islets in the growing type 2 diabetes mellitus (T2DM) rats through the regulation of glucose and fat metabolism. The present study was undertaken
S H Baek et al.
Journal of food science, 75(2), H49-H53 (2010-05-25)
D-psicose has been implicated in glycemic control in recent animal and human studies. In this study, the effects of D-psicose on glycemic responses, insulin release, and lipid profiles were compared with those of D-glucose and D-fructose in a genetic diabetes
Jin-Geun Choi et al.
Applied and environmental microbiology, 77(20), 7316-7320 (2011-08-30)
The S213C, I33L, and I33L S213C variants of D-psicose 3-epimerase from Agrobacterium tumefaciens, which were obtained by random and site-directed mutagenesis, displayed increases of 2.5, 5, and 7.5°C in the temperature for maximal enzyme activity, increases of 3.3-, 7.2-, and
Longtao Zhang et al.
Biotechnology letters, 31(6), 857-862 (2009-02-12)
A non-characterized gene, previously proposed as the D-tagatose-3-epimerase gene from Rhodobacter sphaeroides, was cloned and expressed in Escherichia coli. Its molecular mass was estimated to be 64 kDa with two identical subunits. The enzyme specificity was highest with D-fructose and
Sara E Ali et al.
Molecules (Basel, Switzerland), 24(5) (2019-03-15)
Sugarcane (Saccharum officinarum L.) is an important perennial grass in the Poaceae family cultivated worldwide due to its economical and medicinal value. In this study, a combined approach using mass spectrometry (MS) and nuclear magnetic resonance (NMR) spectroscopy was employed
Soo-Jin Yeom et al.
Applied microbiology and biotechnology, 89(6), 1859-1866 (2010-12-07)
Ribose-5-phosphate isomerase from Clostridium thermocellum converted D-psicose to D-allose, which may be useful as a pharmaceutical compound, with no by-product. The 12 active-site residues, which were obtained by molecular modeling on the basis of the solved three-dimensional structure of the
Sri Rama Krishna Surapureddi et al.
Food chemistry, 281, 36-40 (2019-01-20)
d-Psicose/allulose is a rare sugar and it has high potential benefits for pharmaceutical and food industry. The existed analytical methods have its own limitations to quantify fructose and d-psicose mixtures. Hence there is a need for the development of an
Hye-Jung Kim et al.
Biotechnology letters, 32(1), 113-118 (2009-09-04)
Using site-directed mutagenesis, we investigated the roles of Ile66 and Ala107 of D: -psicose 3-epimerase from Agrobacterium tumefaciens in binding O6 of its true substrate, D: -fructose. When Ile66 was substituted with alanine, glycine, cysteine, leucine, phenylalanine, tryptophan, tyrosine or
Yueming Zhu et al.
Biotechnology letters, 34(10), 1901-1906 (2012-07-05)
The D-psicose 3-epimerase (DPE) gene from Ruminococcus sp. was cloned and overexpressed in Escherichia coli. The recombinant protein was purified and characterized. It was optimally active at pH 7.5-8.0 and 60 °C. Activity was not dependent on the presence of
Zijie Li et al.
Bioorganic & medicinal chemistry letters, 21(23), 7081-7084 (2011-10-25)
It was previously reported that DHAP-dependent aldolase RhaD selectively chooses L-glyceraldehyde from racemic glyceraldehyde to produce l-fructose exclusively. Contrastingly, we discovered that D-glyceraldehyde is also tolerated as an acceptor and the stereoselectivity of the enzyme is lost in the corresponding
Masashi Sato et al.
Biological & pharmaceutical bulletin, 32(5), 950-952 (2009-05-08)
D-Psicose inhibits the growth of L1 stage Caenorhabditis elegans. Sugars, involved in the pentose phosphate pathway, were examined for their ability to reverse the inhibition. Among these sugars, D-ribose specifically exerted reversing activity in a competitive manner. The ingested sugars
Masakazu Harada et al.
Parasitology research, 110(4), 1565-1567 (2011-10-01)
The effects of D-allose and D-psicose on Tritrichomonas foetus were examined. They were cultured in F-bouillon medium including glucose, but had never increased when glucose was substituted to those sugars. When cultured in a medium including a dose of ED(50)
Chia-Jui Lin et al.
Journal of agricultural and food chemistry, 58(19), 10431-10436 (2010-09-09)
L-rhamnose isomerase (EC, L-RhI) catalyzes the reversible aldose-ketose isomerization between L-rhamnose and L-rhamnulose. In this study, the L-Rhi gene encoding L-Rhi was PCR-cloned from Thermoanaerobacterium saccharolyticum NTOU1 and then expressed in Escherichia coli. A high yield of the active
Ingrid S Surono et al.
Nutrition & metabolism, 17, 77-77 (2020-09-25)
The gut microbiota has been shown to be involved in the development and severity of type 2 diabetes. The aim of the present study was to test the effect of 4-week functional food ingredient feeding, alone or in combination, on
Akihito Kano et al.
Journal of plant physiology, 168(15), 1852-1857 (2011-05-24)
We examined rice responses to a rare sugar, d-psicose. Rice growth was inhibited by d-psicose but not by common sugars. Microarray analysis revealed that d-psicose treatment caused an upregulation of many defense-related genes in rice, and dose-dependent upregulation of these
Atsushi Ueda et al.
Carbohydrate research, 345(12), 1722-1729 (2010-06-29)
Disaccharides composed of a beta-D-psicofuranosyl unit were prepared by the glycosylation reaction of monosaccharide acceptors including three 2,3,4,6-tetra-O-protected hexopyranoses with a D-psicofuranosyl benzyl phthalate derivative (4). A beta-D-psicofuranosidic bond was formed by the TMSOTf-promoted reaction with high selectivity. Removal of
Carine Vergne-Vaxelaire et al.
Applied microbiology and biotechnology, 102(13), 5569-5583 (2018-05-08)
Most of the "repressor, open reading frame, kinase" (ROK) proteins already characterized so far, and exhibiting a kinase activity, take restrictedly D-glucose as substrate. By exploring the sequenced bacterial diversity, 61 ATP-dependent kinases belonging to the ROK family have been
Van Duc Long Nguyen et al.
Journal of separation science, 32(11), 1987-1995 (2009-05-30)
Simulated moving bed (SMB) processes have been widely used in the sugar industries with ion-exchange resin as a stationary phase. D-psicose, a rare monosaccharide known as a valuable pharmaceutical substrate, was synthesized by the enzymatic conversion from D-fructose. The SMB
Samir R Dedania et al.
Enzyme and microbial technology, 140, 109605-109605 (2020-09-12)
d-Psicose (d-ribo-2-hexulose or d-allulose) is the Carbon-3 epimer of d-fructose sugar and considered as an unnatural (rare) sugar found in low amount in nature. It has about 70% of the relative sweetness but 0.3% of the energy of sucrose, which
Bioconversion of D-fructose to D-allose by novel isomerases
Bai, W., et al.
Sheng Wu Gong Cheng Xue Bao (Chinese journal of biotechnology), 28, 28-28 (2012)
Patricia Bonin et al.
Environmental science and pollution research international, 22(20), 15347-15359 (2015-01-07)
The impact of petroleum contamination and of burrowing macrofauna on abundances of Marinobacter and denitrifiers was tested in marine sediment mesocoms after 3 months incubation. Quantification of this genus by qPCR with a new primer set showed that the main
Wanmeng Mu et al.
Applied microbiology and biotechnology, 94(6), 1461-1467 (2012-05-10)
D-Psicose is a hexoketose monosaccharide sweetener, which is a C-3 epimer of D-fructose and is rarely found in nature. It has 70 % relative sweetness but 0.3 % energy of sucrose, and is suggested as an ideal sucrose substitute for
Wenli Zhang et al.
Journal of agricultural and food chemistry, 66(22), 5593-5601 (2018-05-16)
d-Allulose is a low-calorie sweetener and has broad applications in the food, cosmetics, and pharmaceutical industries. Recently, most studies focus on d-allulose production from d-fructose by d-allulose 3-epimerase (DAEase). However, the major blocker of industrial production of d-allulose is the
Amelie Rabot et al.
Plant & cell physiology, 53(6), 1068-1082 (2012-04-17)
Bud burst is a decisive process in plant architecture that requires light in Rosa sp. This light effect was correlated with stimulation of sugar transport and metabolism in favor of bud outgrowth. We investigated whether sugars could act as signaling
Tetsuo Iida et al.
Metabolism: clinical and experimental, 59(2), 206-214 (2009-09-22)
Experiments with rats have produced data on the metabolism and energy value of d-psicose; however, no such data have been obtained in humans. The authors assessed the availability of d-psicose absorbed in the small intestine by measuring carbohydrate energy expenditure
Zhou Chen et al.
Bioorganic & medicinal chemistry, 28(10), 115464-115464 (2020-04-07)
A synthetic platform for the cascade synthesis of rare sugars using Escherichia coli whole cells was established. In the cascade, the donor substrate dihydroxyacetone phosphate (DHAP) was generated from glycerol by glycerol kinase (GK) and glycerol phosphate oxidase (GPO). The
Jingxuan Zhu et al.
Frontiers in chemistry, 6, 437-437 (2018-10-16)
The scarcity, richness, and other important physiological functions of D-psicose make it crucial to increase the yield of D-psicose. The production of D-psicose can be accomplished by D-psicose 3-epimerase (DPEase) from Clostridium bolteae (CbDPEase) catalyzing the substrate D-fructose. Although the
Wanmeng Mu et al.
Biotechnology letters, 35(9), 1481-1486 (2013-05-11)
The gene coding for D-psicose 3-epimerase (DPEase) from Clostridium sp. BNL1100 was cloned and expressed in Escherichia coli. The recombinant enzyme was purified by Ni-affinity chromatography. It was a metal-dependent enzyme and required Co(2+) as optimum cofactor. It displayed catalytic
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