Showing 1-30 of 160 results for "P9279"
Z Huang et al.
Biochemistry, 35(12), 3712-3721 (1996-03-26)
Ser-228 has been shown to be essential for the catalytic activity of the human cytosolic phospholipase A2 (cPLA2). However, its involvement in catalysis has not yet been demonstrated. Using site-directed mutagenesis, active-site directed irreversible inhibitors, and the novel fluorogenic substrate...
Chunyan Tan et al.
Arteriosclerosis, thrombosis, and vascular biology, 34(4), 768-778 (2014-02-01)
We previously demonstrated that nicotinamide adenine dinucleotide phosphate oxidase 4 (Nox4) mediates increased monocyte priming and chemotaxis under conditions of diabetic metabolic stress, and emerging data indicate that group VIA phospholipase A2 (iPLA2β) also participates in regulating monocyte chemotaxis. Here...
Ravi S Mishra et al.
The Journal of experimental medicine, 205(2), 347-359 (2008-01-23)
Monocyte chemoattractant protein-1 (MCP-1) directs migration of blood monocytes to inflamed tissues. Despite the central role of chemotaxis in immune responses, the regulation of chemotaxis by signal transduction pathways and their in vivo significance remain to be thoroughly deciphered. In...
Dusko Ilic et al.
Biochimica et biophysica acta, 1841(3), 416-421 (2013-11-26)
The mammalian epidermis provides both an interface and a protective barrier between the organism and its environment. Lipid, processed into water-impermeable bilayers between the outermost layers of the epidermal cells, forms the major barrier that prevents water from exiting the...
Perttu Haimi et al.
The Journal of biological chemistry, 285(1), 751-760 (2009-11-06)
To better understand the principles underlying the substrate specificity of A-type phospholipases (PLAs), a high throughput mass spectrometric assay was employed to study the effect of acyl chain length and unsaturation of phospholipids on their rate of hydrolysis by three...
Structure and properties of a secretable phospholipase A2 from human platelets.
R M Kramer et al.
Advances in experimental medicine and biology, 275, 35-53 (1990-01-01)
Yoshitaka Taketomi et al.
Nature immunology, 14(6), 554-563 (2013-04-30)
Microenvironment-based alterations in phenotypes of mast cells influence the susceptibility to anaphylaxis, yet the mechanisms underlying proper maturation of mast cells toward an anaphylaxis-sensitive phenotype are incompletely understood. Here we report that PLA2G3, a mammalian homolog of anaphylactic bee venom...
T Minami et al.
Biochimica et biophysica acta, 1170(2), 125-130 (1993-10-13)
We purified a phospholipase A2 (PLA2) from human ileal mucosa to homogeneity. Its NH2-terminal amino acid sequence, amino acid composition, molecular weight, and elution behavior on reverse phase high-performance liquid chromatography were identical to those of human group II PLA2...
L L Lin et al.
Cell, 72(2), 269-278 (1993-01-29)
Treatment of cells with agents that stimulate the release of arachidonic acid causes increased serine phosphorylation and activation of cytosolic phospholipase A2 (cPLA2). Here we report that cPLA2 is a substrate for mitogen-activated protein (MAP) kinase. Moreover, phosphorylation by MAP...
PLA2G3 promotes mast cell maturation and function.
Philipp Starkl et al.
Nature immunology, 14(6), 527-529 (2013-05-21)
Distribution of reaction products in phospholipase A2 hydrolysis.
Wacklin HP
Biochimica et Biophysica Acta, 1768(5), 1036-1049 (2007)
Cedric H De Smet et al.
Biochimica et biophysica acta, 1831(6), 1167-1176 (2013-03-19)
In the yeast Saccharomyces cerevisiae, the molecular species profile of the major membrane glycerophospholipid phosphatidylcholine (PC) is determined by the molecular species-selectivity of the biosynthesis routes and by acyl chain remodeling. Overexpression of the glycerol-3-phosphate acyltransferase Sct1p was recently shown...
J Chen et al.
The Journal of biological chemistry, 269(4), 2365-2368 (1994-01-28)
Extensive biochemical studies of phospholipase A2s (PLA2s) over the last two decades indicate that there are likely to be several distinct PLA2 genes in mammals. Here we report the cloning of a 1-kilobase pair cDNA encoding a novel human low...
Biochemical and functional characterization of a new recombinant phospholipase A2 inhibitor from Crotalus durissus collilineatus snake serum.
Gimenes, et al.
International Journal of Biological Macromolecules, 164, 1545-1553 (2021)
Ming Chen et al.
Scientific reports, 7(1), 15931-15931 (2017-11-23)
Hepatitis C virus (HCV), dengue virus (DENV) and Japanese encephalitis virus (JEV) belong to the family Flaviviridae. Their viral particles have the envelope composed of viral proteins and a lipid bilayer acquired from budding through the endoplasmic reticulum (ER). The...
Irina Nazarenko et al.
Journal of cell science, 120(Pt 8), 1393-1404 (2007-03-22)
HRSL3 (also known as H-REV107-1) belongs to a class II tumor suppressor gene family and is downregulated in several human tumors including ovarian carcinomas. To unravel the mechanism of HRSL3 tumor suppressor action, we performed a yeast two-hybrid screen and...
Xiaobai Ren et al.
FEBS letters, 584(19), 4222-4226 (2010-09-15)
H-REV107 is a Ca(2+)-independent phospholipase A(1/2), and it is also a pro-apoptosis protein belonging to the novel class II tumor suppressor family, H-REV107-like family. Here we report the solution structure of the N-terminal catalytic domain of human H-REV107, which has...
K Kitadokoro et al.
Journal of biochemistry, 123(4), 619-623 (1998-05-21)
Phospholipase A2 is a key enzyme in a number of physiologically important cellular processes including inflammation and transmembrane signaling. Human secretory phospholipase A2-IIA is present at high concentrations in synovial fluid of patients with rheumatoid arthritis and in the plasma...
S Siegrist et al.
Oncogene, 20(37), 5155-5163 (2001-08-30)
By systematic analysis of a human testis library, we have isolated the hH-Rev107-3 cDNA, identical to hH-Rev107-1 cDNA, which was previously described as a class II tumor suppressor gene. In this study, two transcripts (1 and 0.8 kb) were detected...
Hafid Ait-Oufella et al.
Arteriosclerosis, thrombosis, and vascular biology, 33(3), 466-473 (2013-01-26)
Several secreted phospholipases A2 (sPLA2s), including group IIA, III, V, and X, have been linked to the development of atherosclerosis, which led to the clinical testing of A-002 (varespladib), a broad sPLA2 inhibitor for the treatment of coronary artery disease....
N Suzuki et al.
The Journal of biological chemistry, 275(8), 5785-5793 (2000-02-22)
Mammalian secretory phospholipase A(2)s (sPLA(2)s) form a family of structurally related enzymes that are involved in a variety of physiological and pathological processes via the release of arachidonic acid from membrane phospholipids or the binding to specific membrane receptors. Here...
Hilary F Clark et al.
Genome research, 13(10), 2265-2270 (2003-09-17)
A large-scale effort, termed the Secreted Protein Discovery Initiative (SPDI), was undertaken to identify novel secreted and transmembrane proteins. In the first of several approaches, a biological signal sequence trap in yeast cells was utilized to identify cDNA clones encoding...
Marcin Golczak et al.
The Journal of biological chemistry, 287(28), 23790-23807 (2012-05-19)
Lecithin:retinol acyltransferase-like proteins, also referred to as HRAS-like tumor suppressors, comprise a vertebrate subfamily of papain-like or NlpC/P60 thiol proteases that function as phospholipid-metabolizing enzymes. HRAS-like tumor suppressor 3, a representative member of this group, plays a key role in...
Reply: limits of Mendelian randomization analyses in selection of secretory phospholipase A2-IIA as a valid therapeutic target for prevention of cardiovascular disease.
Michael V Holmes et al.
Journal of the American College of Cardiology, 63(9), 943-943 (2013-12-10)
Chandra D Naveen et al.
Interdisciplinary sciences, computational life sciences, 4(4), 268-272 (2013-01-29)
The anti inflammatory potential of the human and microbial biotransformed derivatives of berberine were determined by molecular docking. It was revealed that almost all derivatives formed as a result of biotransformation showed increase in phospholipase A(2) binding affinity compared to...
Thomas R Burkard et al.
BMC systems biology, 5, 17-17 (2011-01-29)
On the basis of large proteomics datasets measured from seven human cell lines we consider their intersection as an approximation of the human central proteome, which is the set of proteins ubiquitously expressed in all human cells. Composition and properties...
Shan-Shan Qin et al.
Biochemistry, 52(8), 1477-1489 (2013-01-25)
Human secreted phospholipase A2s have been shown to promote inflammation in mammals by catalyzing the first step of the arachidonic acid pathway by breaking down phospholipids, producing fatty acids, including arachidonic acid. They bind to the membrane water interface to...
J D Clark et al.
Cell, 65(6), 1043-1051 (1991-06-14)
We report the cloning and expression of a cDNA encoding a high molecular weight (85.2 kd) cytosolic phospholipase A2 (cPLA2) that has no detectable sequence homology with the secreted forms of PLA2. We show that cPLA2 selectively cleaves arachidonic acid...
Ladeana W Hillier et al.
Nature, 434(7034), 724-731 (2005-04-09)
Human chromosome 2 is unique to the human lineage in being the product of a head-to-head fusion of two intermediate-sized ancestral chromosomes. Chromosome 4 has received attention primarily related to the search for the Huntington's disease gene, but also for...
Stephen J Nicholls et al.
JAMA, 311(3), 252-262 (2013-11-20)
Secretory phospholipase A2 (sPLA2) generates bioactive phospholipid products implicated in atherosclerosis. The sPLA2 inhibitor varespladib has favorable effects on lipid and inflammatory markers; however, its effect on cardiovascular outcomes is unknown. To determine the effects of sPLA2 inhibition with varespladib...

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