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Showing 1-21 of 21 results for "PCR multiwell plates"
Standard Reverse Transcription Protocol (Two-step)
reverse transcription is the analysis of gene expression to measure concentration mrna a gene. there are several challenges such analyses, as differences in halflife between different transcripts, temporal patterns and lack correlation protein.
qPCR Gene Expression Using Probe Detection
The most common application for qPCR is the measurement of a gene transcript or copy number quantity relative to one or more reference genes using probe detection.
Reverse Transcription Protocol (One-step Probe Detection)
Reverse transcription (RT) is the process of converting RNA to cDNA using a reverse transcriptase enzyme and dNTPs.
dNTP Mediated Hot Start PCR Protocol
Hot Start dNTPs are modified with a thermolabile protecting group at the 3’ terminus. The presence of this modification blocks nucleotide incorporation by DNA polymerase until the nucleotide protecting group is removed during a heat activation step.
REDExtract-N-Amp™ Seed PCR Kit Protocol
The REDExtract-N-Amp Seed PCR Kit contains all the reagents needed to rapidly extract and amplify genomic DNA from seeds (soybean, corn, wheat, etc.).
DNA Amplification using Illustra PuReTaq Ready-To-Go PCR Beads
This page shows DNA amplification using illustra PuReTaq ready-to-go PCR beads from Cytiva.
Extract-N-Amp™ FFPE Tissue PCR Protocol
The following procedure is a modification of the Extract-N-Amp™ Tissue PCR protocol.
Extract-N-Amp™ FFPE Tissue PCR Protocol
Protocol for PCR amplification of formalin-fixed paraffin embedded animal tissues using the Extract-N-Amp Tissue PCR kit.
AptaTaq Fast DNA Polymerase Protocol & Troubleshooting
AptaTaq Fast DNA Polymerase Protocol & Troubleshooting
Extract-N-Amp™ Tissue PCR Kit Protocol
The Extract-N-Amp™ Tissue PCR Kit contains all the reagents needed to rapidly extract and amplify genomic DNA from mouse tails and other animal tissues, buccal swabs, hair shafts, and saliva.
REDExtract-N-Amp™ Tissue PCR Kit Protocol
The exrract-N-Amp kit protocol provides a rapid DNA extraction method that leads to a PCR-ready sample in 15 minutes. Optimized PCR ReadyMix yields successful and clean amplification. RED loading dye allows for sample tracking.
SYBR® Green Extract-N-Amp™ Tissue PCR Kit Protocol
The SYBR® Green Extract-N-Amp™ Tissue PCR Kit contains all the reagents needed for rapid extraction, amplification and detection of genomic DNA from mouse tails and other animal tissues, buccal swabs, hair shafts, and saliva.
Standard PCR Protocol
Learn standard PCR protocol steps and review reagent lists or cycling parameters. This method for routine PCR amplification of DNA uses standard Taq DNA polymerase.
Buccal DNA Extraction & WGA Amplification Protocol
This protocol provides a simple and convenient method to isolate, amplify, and purify genomic DNA from buccal swabs. Buccal swabs are a convenient method of acquiring a DNA sample.
Protocol for DNA extraction & WGA Amplification from Soil Samples
Genomic DNA from soil samples can be easily damaged by nucleases and contaminating debris resulting in low DNA yields. As a result, the researcher’s ability to perform downstream analysis may be compromised. After isolating DNA from the soil sample, the...
Biological Sample Collection, Transport, Archiving, and Purification for Nucleic Acid Analysis
A number of options are available for sample collection, transport, archiving, and DNA purification.
Extraction & Amplification of whole blood using WGA-Protocol
Whole blood is a common source of material used to perform genetic analysis. Many times genomic DNA isolated from whole blood samples is of low yield. This protocol is a simple method to isolate DNA from fresh or aged...
Saliva DNA Extraction & WGA Amplification Protocol
Whole Genome Amplification can be performed on DNA extracted in many ways. We offer many products for DNA extraction, including the GenElute™ Blood Genomic DNA Kit, GenElute Mammalian Genomic DNA Miniprep Kit and the GenElute Plant Genomic DNA M iniprep.
Blood Card - Extraction & Amplification WGA Protocol
Blood cards provide the convenience of archiving small volumes of blood. However, many times genomic DNA from these samples is limited, This protocol provides a simple and convenient method to extract genomic DNA from a blood card. Once the DNA...
GenomePlex Whole Genome Amplification Kit Protocol (WGA1)
GenomePlex is a Whole Genome Amplification (WGA) method that allows the researcher to generate a representative, 500-fold amplification of genomic DNA
Plant DNA Extraction & WGA Amplification Protocol
GenomePlex® Whole Genome Amplification has been used to amplify genomic DNA from soybean, corn, tomato, purple coneflower, and ginseng.

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