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Casein Blocking Buffer for Southern or Western Blotting
Unoccupied sites on the solid support must be blocked to detect an immobilized antigen. This protocol describes how to use our Casein Blocking Buffer in Southern and Western blotting applications, including options for fluorescein and DIG detection systems.
Gelatin Blocking Buffer for Direct Detection Southern or Western Blotting
In order to specifically detect an antigen or target molecule immobilized on a solid support, unoccupied binding sites on the support must be blocked against binding by probe and detection molecules.
Antibody Purification using Protein A, Protein G, or Protein L Agarose
Antibody Purification using Protein A, Protein G, or Protein L Agarose protocol is designed as a quick purification method for antibodies from mammalian sera, ascites, and cell culture supernatants. It should be noted that if the starting material is serum
Harvesting Cells from Corning® CellSTACK® Culture Chambers
This technical article covers the protocol for how to properly harvest cells from Corning® CellSTACK® culture chambers.
Preparation of FSL-Tyr1 Construct Protocol
FSL-Tyr1 is comprised predominantly of an N-formyltyrosine monomer, which can be radiolabeled with 125I
ELISA Procedures
ELISA protocols providing detailed instructions on performing indirect ELISA and Capture ELISA . Learn more about recommended products and techniques for performing both sandwich ELISA and indirect ELISA experiments.
Calcium Phosphate Transfection Kit Protocol
Calcium phosphate transfection is a common method for the introduction of DNA into eukaryotic cells. This protocol can be optimized for use with a wide variety of cell types.
HDM-PAMPA with the MultiScreen Permeability Filter Plate
HDM-PAMPA with the MultiScreen Permeability Filter Plate
Sera-Mag™ SpeedBeads Magnetic Protein A/G-Particles Protocol
Sera-Mag SpeedBeads Protein A/G Magnetic Particles provide a fast and convenient method for both manual and automated magnetic isolation of proteins using affinity binding. The particles can be used for isolating antibodies from serum, cell culture supernatant or ascites, and
Stripping and Reprobing Western Blotting Membranes
This page shows and discusses three protocols for stripping and reprobing a western blot membrane.
Immunofluorescence Labeling of Cells
Cell staining can be divided into four steps: cell preparation, fixation, application of antibody, and evaluation.
Two-step Purification of Mouse Monoclonal IgG1 Using HiTrap® Protein G HP for the Capture Step
This page shows how to perform a two-step purification of mouse monoclonal IgG1 using HiTrap® Protein G HP from Cytiva for the capture step.
Toluidine Blue Staining Protocol for Millicell® Culture Inserts
This is a Toluidine Blue Staining protocol listing materials and methods.
Cultrex® 3-D Culture Matrix™ Rat Collagen I Protocol
3-D Culture Matrix™ Collagen I may be used as a gel on which to grow cells or a media additive alone or in concert with other basement membrane components to study cellular growth and differentiation in three dimensions in vitro.
Key Steps in Flow Cytometry Protocols
Learn key steps in flow cytometry protocols to make your next flow cytometry experiment run with ease.
Biodegradable Nanoparticles
Preparation for biodegradable nanoparticles and their use in transfection protocols .
Cell Capture Imaging Reagent
Immunocytochemistry (ICC) Cell Capture Imaging Reagent and protocol for eliminating wash steps, and is highly effective in applications involving rare cells, low cell count samples, and limited access samples.
Column Packing and Preparation Protocol for Affinity Chromatography
This page shows how to perform column packing and preparation for affinity chromatography when using Tricorn™ or XK columns available from Cytiva.
HL-1 Cells
We’ve got everything else you’ll need to get started in your research of your HL-1 cells. From culture plates and cryovials to the proprietary Claycomb Media, look no further for a complete offering of products and services to support your
Human Skeletal Muscle Cells (HSkMC) Culture Protocol
Technical information for working with human skeletal muscle cells including thawing, subculturing and cryopreservation
30-minute Immunodetection Protocol Using the SNAP i.d.® 2.0 System
The SNAP i.d. 2.0 Protein Detection System is the second generation of the SNAP i.d. method for detecting immunoreactive proteins on Western blots.
Analysis of Aflatoxin B1, B2, G1, and G2 in Corn Meal, Wheat Meal, and Raw Peanut Paste Matrices
Supel™ Tox AflaZea SPE cartridges provide exceptional cleanup of corn meal, wheat meal, and raw peanut paste, yielding high analyte recoveries and low %RSD for aflatoxin analysis.
3-Step ColorWheel® Flow Cytometry Reagent Preparation Protocol
Experience simplicity in your flow cytometry workflow with the 3-Step ColorWheel® Flow Cytometry Reagent Preparation protocol. With less than 5 minutes of hands-on time, see how simple it is to create your own optimal flow cytometry reagents with ColorWheel® flow
Column Packing and Preparation for Affinity Chromatography with Specific Groups of Biomolecules
Column packing and preparation for Affinity Chromatography with specific groups of biomolecules.
Column Packing and Preparation for Affinity Chromatography with Specific Groups of Biomolecules
Column packing and preparation for Affinity Chromatography with specific groups of biomolecules.
Comet Assay
The Comet Assay, also called single cell gel electrophoresis (SCGE), is a sensitive and rapid technique for quantifying and analyzing DNA damage in individual cells.
How to use Scepter™ 2.0 Cell Counter
The Scepter™ cell counter uses the Coulter principle of impedance-based particle detection to reliably and accurately count every cell in your sample.
Column Packing and Preparation
This page describes efficient column packing and preparation for affinity chromatography of tagged proteins using Cytiva products.
Column packing and preparation for Ion Exchange Chromatography & Chromatafocusing
This page shows how to perform column packing and preparation for ion exchange chromatography and chromatafocusing when using Tricorn or XK columns available from Cytiva.
Detergent Removal with Amicon® Pro Centrifugal Filters
Detergents are frequently used to solubilize proteins and nucleic acids during purification, but the presence of detergents may interfere with downstream analyses.
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