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BacMagic Systems pIEx/Bac Expression Vectors
pIEx/Bac™-1 DNA 20 µg 71724-3 pIEx/Bac™-1 Ek/LIC Vector Kit 20 rxn 71729-3
pIEx/Bac™-3 DNA 20 µg 71726-3 pIEx/Bac™-3 3C/LIC Vector Kit 20 rxn 71731-3
pIEx/Bac™-4 DNA 20 µg 71727-3 pIEx/Bac™-4
TB163 Ek/LIC Cloning Kits
Promoter Primer pIEx™-1, pIEx™-7, pIEx™-8, pIEx™-10, pIEx/Bac™-1,
pIEx/Bac™-4
69103-3
S•Tag™ 18mer Primer pET-30, pET-32, pET-43.1, pET-44, pIEx™-1, pIEx™-2,
pIEx™-3, pTriEx™-4
See note
Express yourself
Infection, pIEx/Bac-4, CHK1 (81.6 kDa)
2 Transfection, pIEx/Bac-4, CHK1 (81.6 kDa)
3 Infection, pIEx/Bac-5, CHK1 (83.9 kDa)
4 Transfection, pIEx/Bac-5, CHK1 (83.9 kDa)
5 Infection, pIEx/Bac-4, Rluc (64.3
inNovations Newsletter #16
Cat. No.
pIEx™-1 DNA 20 µg 71241-3
pIEx-2 DNA 20 µg 71238-3
pIEx-3 DNA 20 µg 71243-3
pIEx-4 DNA 20 µg 71235-3
pIEx-5 DNA 20 µg 71242-3
pBiEx™-1 DNA 20 µg 71234-3
pBiEx-2
TB356 InsectDirect™ pIEx™ Vectors
InsectDirect™ pIEx™-1 DNA 20 µg 71241-3
InsectDirect pIEx-2 DNA 20 µg 71238-3
InsectDirect pIEx-3 DNA 20 µg 71243-3
InsectDirect pIEx-4 DNA 20 µg 71235-3
InsectDirect pIEx-5 DNA 20 µg 71242
TB348 pIEx™-2 Vector Map
II (2390)
Nco I (1082)
Tth111 II (2327)
pIEx-2
(4563 bp)
pIEx-2 cloning/expression region
Novagen
Cat. No.
pIEx-2 DNA 71238-3
pIEx-2 Ek/LIC Vector 71239-3
Enzyme #
Protocol - 71259
CytoBuster™,
pBiEx™, pIeX™, TriEX™, Reportasol™, the Novagen® logo and Novagen name are trademarks of EMD Chemicals, Inc. in the United States and in certain
other jurisdictions. pIEx and pBiEx Vectors
Poster: Montage BAC Miniprep Kit: New Method for High Throughput BAC DNA Purification; GSAC, 2001
provides DNA of sufficient quantity and purity for direct BAC end sequencing.
Cultivating the BAC clones in a 96 well culture block, we are able to reproducibly isolate 0.5-
1.0 mg of BAC DNA per well
Poster: Increasing throughput and quality of BAC-end Sequencing by Automation...2001
electrophoresis of a sample of 3 BAC DNA samples that were left uncut
(lanes 1-3), digested with NotI (lanes 4-6) or with HindIII (lanes 7-9)
A.
Electropherogram of
BAC end Sequence
0
2
4
6
8
10
12
14
16
Montage BAC 96 Miniprep Kit
neutralized lysate filtration is essential for optimal yield
of BAC DNA.
Variable BAC DNA Highly variable BAC DNA Molar concentrations of BAC DNA
yields inserts size (50–300 Kb) will be equivalent and adequate
Montage Plasmid MiniprepHTS Kit User Guide
neutralized lysate filtration is essential for optimal yield
of BAC DNA.
Variable BAC DNA Highly variable BAC DNA Molar concentrations of BAC DNA
yields inserts size (50–300 Kb) will be equivalent and adequate
Radiance! Multisystem Protein Expression
✕ ✕ Tb/Ek 70051-3 $343
pIEx™-1 Ek/LIC3 ✕ ✕ ✕ ✕ Tb/Ek 71237-3 $343
pIEx-2 Ek/LIC3 ✕ ✕ ✕ ✕ ✕ Tb/Ek 71240-3 $343
pIEx-3 Ek/LIC3 ✕ ✕ ✕ ✕ ✕ ✕ Tb/Ek 71245-3
Product Information Sheet - NA0100
PhasePrep BAC DNA Kit (NA0100) - Technical Bulletin
PhasePrep™ BAC DNA Kit
Catalog Number NA0100
TECHNICAL BULLETIN
Product Description
The PhasePrep BAC DNA Kit offers a scalable and
cost-effective
Transfection: A Science and an Art
transfection
of Sf9 insect cells when using with
pIEx™ and pIEx/Bac™ vectors.
Feature Benefit
Suitable for both stable and transient transfections with
plasmid DNA as well as cotransfection for recombinant
DNA Template Prep and Sequencing Reaction Cleanup
concentrate BAC DNA on the BAC plate
membrane surface. Contaminants are filtered
to waste. Add wash solution and apply
vacuum (4 – 6 min) to wash.
3. Add resuspension solution and collect purified
BAC DNA by
MultiScreen HTS PLASMID 96-Well Plates User Guide
NOTE: Freezing the pellets improves resuspension and
yield of BAC DNA.
3. Allow the pellets to thaw at room temperature for 15 minutes.
4. Resuspend pellets by adding 100 µL of Solution 1 (contain-
Preparation and Sequencing of Fosmid DNA Templates using the Montage BAC96 Miniprep Kit and Montage SEQ96 & SEQ384 Clean-up Kits and Plates
3
13. Pipette retained fosmid DNA from the wells of the BAC plate into the V-bottom plate for
storage. (Recovered volume can be maximized by tilting the BAC plate before collecting
the sample.)
inNovations Newsletter #18
Figure 3. pIEx-6 vector map
pIEx-6
www.novagen.com
Product Size Cat. No.
pET-45b(+) DNA 10 µg 71327-3
pCDF-1b DNA 10 µg 71330-3
pRSF-1b DNA 10 µg 71363-3
pIEx™-6 DNA 20 µg 71333-
Protein Purification and Detection Tools
- easier!
• N pIEx/Bac™ Vectors
One vector, two methods:
plasmid-based or baculovirus
• InsectDirect™ System
Plasmid-based protein expression
in 48 hours
• BacMagic™ DNA
Baculovirus
Montage Plasmid Miniprep96 Kit
All rights reserved.
Purification/
Cleanup of DNA
Template DNA
Plasmid DNA
BAC DNA
PCR DNA
Montage
Solutions for
PCR, Plasmids,
and BACs
Electrophoresis
Gel
Extraction
Montage
Gel
Product Information Sheet - M8818
1100 1 X
Genomic DNA x (2.2 – 5.5 µg
total)
20 – 50 ng
/reaction
PCR grade
water
660 – x
Total volume 1760
Product Information Sheet - M4193
P1107)
1100 1X
Genomic DNA x (2.2-5.5 µg
total)
20-50 ng
PCR grade
water
660-x
Total volume 1760
MultiScreen PCR µ96 Plate
Manifold SAVM 384 01 1
Also Available
MultiScreen Plasmid
96
Plate LSKP 096 24 24
MultiScreen BAC
96
Plate LSKB 096 24 24
MultiScreen SEQ
96
Plate LSKS 096 24 24
MultiScreen SEQ
384
Plate
User Guide-LSKSBW500
has the most dramatic effect on sequencing quality. For optimal results, we recommend that
plasmid, BAC and PCR templates be prepared with Montage kits.
2 Efficacy has been demonstrated at template amounts
Manual: E. cloni® 10G and 10GF’ Electrocompetent Cells
propagation of BAC, cosmid, or plasmid clones. They
give high yield and high quality plasmid DNA due to the endA1 mutation.
E. cloni ® 10G and 10GF´ Electrocompetent
High fidelity gene amplification
(lanes 1 – 3) or after
digestion with Not I (lanes 4 – 6) or Hind III (lanes
7 – 9). Lane M is a Hind III digest of Lambda
phage DNA. The BAC DNA purified with the
Montage® Plasmid
Whole Genome Amplification Product Listing
Figure 2. DNA from normal and tumorigenic human kidney cells were
amplified using the GenomePlex Single Cell WGA Kit. Amplified material
was hybridized to metaphase BAC arrays to determine
Bulletin - HUMANVS
promoter/enhancer regions that control
transcription.
2. Walking from both directions into unknown BAC terminal sequencing, for a greater
distance than traditional sequencing allows.
3. Allowing the
inNovations Newsletter #15
vectors
(22). Additionally, cloning DNA sequences
in the single-copy state is advantageous for
reducing rearrangements, deletions and
other mutations as demonstrated with BAC
(Bacterial Artificial Chromosome
Bulletin - RATVS
of the targeted PCR amplicon. Hot start can be achieved by using
JumpStart REDAccuTaq DNA polymerase.
4
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