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Poly-lysine | Poly-D-Lysine | Poly-L-Lysine
Poly-Lysine enhances electrostatic interaction between negatively-charged ions of the cell membrane and positively-charged surface ions of attachment factors on the culture surface. When adsorbed to the culture surface, it increases the number of positively-charged sites available for cell binding.
Poly-L-Lysine Cell Attachment Protocol
Polyamino acids facilitate the attachment of cells and proteins to solid surfaces in biological applications. In cell cultures normal attachment, growth, and development of many cell types are dependent on attachment factors and extracellular matrix components.
Laminin Coating Protocol
Laminin coating protocol, products and FAQs at sigmaaldrich.com
Attachment Factors
Attachment factors mimicking the extracellular matrix (ECM) provide important cues to cultured cells that stimulate physiological growth and behavior. A wide array of biological and synthetic polymers can be selected to enhance cell culture conditions.
Cell Growth & Maintenance
A synopsis of the conditions, media, nutrients, cultureware, and environments required for successful propagation of mammalian and yeast cells. Nutrient reagents, sterile technique and filtration, and requirements for 2D, 3D, and suspension cultures are described.
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Take advantage of your EVERYDAY UC pricing and receive FREE FREIGHT*on top selling Cell Culture Reagents. Reference promo code UMV when placing your order through June 25, 2021.
Extracellular Matrix Proteins and Tools for Cell Culture Optimization
Extracellular matrix proteins such as laminin, collagen, and fibronectin can be used as cell attachment substrates in cell culture.
Lentiviral Production Using X-tremeGENE HP Transfection Reagent
Lentiviruses represent a powerful tool in research applications to transduce a wide range of cell types.
Complex APIs
Our API contract manufacturing services can support all stages of your project. Partner with an industry leader offering a global footprint and decades of experience for your drug development and manufacturing, leveraging our dedicated team and broad product portfolio with...
Tailoring Collagen-Based Matrices for Regenerative Medicine and Tissue Engineering
Collagen molecules play a critical role in tissue architecture and strength, and in cell-matrix interactions as insoluble ligands to regulate the diverse phenotypic activities of cells.
Tissue Diagnostics Universal Reagents
Discover our comprehensive range of reliable products used for histological microscopic analysis including reagents and labware for sample preparation, fixation, staining, mounting and diagnostics.
Histology Stains
Discover the complete line of routine and special histological stains, control slides and other staining supplies for general histology and histopathology.
Hematology Stains
Reliably differentiate and diagnose blood and bone marrow samples with your preferred protocol by selecting from our full range of high-quality hematological staining solutions.
ELISA Protocols
This article offers 4 popular ELISA protocols: Sandwich ELISA protocol, Phosphorylation Assay Procedure, EIA Assay Procedure, & Cell-based Assay Procedure.
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Save up to 90% and receive FREE FREIGHT* on top selling Life Science Reagents.
Derivation of Functional Oligodendrocyte Progenitor Cells (OPCs) from Human Neural Stem Cell Lines
Derivation and characterization of functional human neural stem cell derived oligodendrocyte progenitor cells (OPCs) that efficiently myelinate primary neurons in culture.
Nanoparticle-Based Drug and Gene Delivery for Tumor Targeting
Professor Yoshiki Katayama (Kyushu University, Japan) discusses recent advances in drug delivery systems and strategies that exploit the EPR effect, with a special focus on stimuli-responsive systems based on novel materials.
Recent Advances on Nanoparticle-Based Imaging Contrast Agents for in Vivo Stem Cell Tracking
Professor Yu Cheng and co-workers look at the recent advances of nanoparticle-based imaging contrast agents for in vivo stem cell tracking. They provide an in-depth look at popular in vivo imaging techniques, magnetic resonance imaging, fluorescence imaging, ultrasound imaging, and...
Protein- and Peptide- Polymer Conjugates by RAFT Polymerization
The modification of biomacromolecules, such as peptides and proteins, through the attachment of synthetic polymers has led to a new family of highly advanced biomaterials with enhanced properties.
Chemistry in (Bio)Materials Science
"Click" Chemistry in (Bio)Materials Science
Smart Oligonucleotide Delivery by Multifunctional Block Copolymers
Kanjiro Miyata (The University of Tokyo, Japan) provides insights on the rational design of polymeric materials for “smart” oligonucleotide delivery.
Mammalian Cell Culture
Applications, principles, and methods for in vitro mammalian cell culture including links to articles, protocols, and videos for proper aseptic technique, passaging and subculturing, and culture media considerations.
CellASIC® ONIX live cell analysis platform for neural stem cell microenvironment control
This article describes CellASIC® ONIX live cell analysis platform for neural stem cell microenvironment control
Polyethylene Glycol Building Blocks for PEGylation
Sigma-Aldrich.com presents an article regarding Polyethylene Glycol Building Blocks for PEGylation.
Western Blotting: Why Are Observed and Calculated Molecular Weights Different?
A discussion of factors affecting observed vs. calculated molecular weight in gel electrophoresis and western blotting.
Fouling Resistant Biomimetic Poly(Ethylene Glycol) Based Grafted Polymer Coatings
Fouling Resistant Biomimetic Poly(Ethylene Glycol) Based Grafted Polymer Coatings
Synthesis and Biomedical Applications of Polyamino Acids
Synthesis and Biomedical Applications of Polyamino Acids
Applications of Glucose Polymers in the Synthesis of Diagnostic and Drug Delivery Nanoparticles
Dmitri Simberg (University of Colorado Anschutz Medical Campus, USA) reviews the used of dextran and cyclodextrin for the synthesis of nanoparticles used in drug delivery applications.
Hematology
Learn about the clinical study of blood, blood-forming organs, and blood diseases including the treatment, prevention, and stains and dyes used in hematology testing.
Immunohistochemistry
Immunohistochemistry (IHC) is a common immunostaining technique that is used by researchers to identify specific antigens of interest in healthy and diseased tissues.
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