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Potassium phosphate monobasic

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Enzymatic Assay of Ficin (EC 3.4.22.3)
This procedure may be used for all Ficin products.
Enzymatic Assay of Ficin (EC 3.4.22.3)
This procedure may be used for all Ficin products.
Spectrophotometric Determination of Reinheitszahl (RZ) for Peroxidase (EC 1.11.1.7)
Reinheitszahl (RZ) is the ratio of absorbance due to hemin (A403, Soret region) to absorbance due to protein (A275).
Enzymatic Assay of Malic Dehydrogenase (EC 1.1.1.37)
This is a protocol to be used for all Malic Dehydrogenase products.
Enzymatic Assay of Lyticase
This procedure may be used for the determination of Lyticase activity using Baker’s yeast as the substrate.
Enzymatic Assay of Lysozyme (EC 3.2.1.17)
This enzymatic rate determination may be used for Lysozyme products. It is not to be used to assay recombinant or insoluble Lysozyme on agarose.
Enzymatic Assay of Catalase (EC 1.11.1.6)
This procedure may be used for all Catalase products.
Enzymatic Assay of Peroxidase (EC 1.11.1.7)
This procedure is for the determination of Peroxidase enzymatic activity using Pyrogallol as the substrate.
Enzymatic Assay of Alcohol Oxidase (EC 1.1.3.13)
This procedure may be used for all Alcohol Oxidase products. The continuous spectrophotometric rate determination (A405, Light path = 1 cm) is based on the following reactions.
Enzymatic Assay of Peroxidase (EC 1.11.1.7) 2,2'-Azino-bis(3-Ethylbenzthiazoline-6-Sulfonic Acid) as a Substrate
To standardize a procedure for the assay of Peroxidase using 2,2'-Azino-bis(3-Ethylbenzthiazoline-6-Sulfonic Acid) as a substrate.
Enzymatic Assay of Laccase (EC 1.10.3.2)
Laccase (EC 1.10.3.2) is a cuproenzyme that oxidizes various types of phenols and similar aromatic compounds aromatic amines with the reduction of molecular oxygen to water, therefore, is used as a biocatalyst.
USP Dissolution Testing Method (HPLC) for Folic Acid Tablets Using a Monolith Column and UV detection
Folic acid or folate is classified as a B vitamin (B9). Folic acid is synthetically produced, and used in fortified foods and supplements. Folate is converted by humans to dihydrofolate (dihydrofolic acid), tetrahydrofolate (tetrahydrofolic acid), and other derivatives, which have various biological
Enzymatic Assay of 3α-Hydroxysteroid Dehydrogenase with Androsterone (EC 1.1.1.50)
Enzymatic Assay of 3α-Hydroxysteroid Dehydrogenase with Androsterone (EC 1.1.1.50)
Phycology Media in Plant Tissue Culture
Phycology media can be prepared from concentrated solutions or from powdered salt mixtures. The concentrated solutions are complete, including vitamins, and should be stored frozen.
Enzymatic Assay of α-Amylase (EC 3.2.1.1)
Follow our procedure for the determination of alpha-Amylase activity. This enzymatic assay of a-Amylase guides you through the entire process and necessary calculations.
Enzymatic Assay of Proteinase K (EC 3.4.21.64)
Proteinase K (EC 3.4.21.64) activity can be measured spectrophotometrically using hemoglobin as the substrate. Proteinase K hydrolyzes hemoglobin denatured with urea, and liberates Folin-postive amino acids and peptides. One unit will hydrolyze hemoglobin to produce color equivalent to 1.0 μmol
Enzymatic Assay of Plasmin with D-Val-Leu-Lys-p-Nitroanilide Dihydrochloride
Enzymatic Assay of Plasmin with D-Val-Leu-Lys-p-Nitroanilide Dihydrochloride
RNAi In Situ
In Situ Hybridization of Whole-Mount Mouse Embryos with RNA Probes: Hybridization, Washes, and Histochemistry. This is a protocol describing how to perform in situ hybridization on whole mouse embryos. Here we describe the hybridization procedure, and the localization of the
Enzymatic Assay of Fumarase
Enzymatic Assay of Fumarase
Enzymatic Assay of Lipase Type XIII from Pseudomonas sp. Using a Coupled Enzyme System of Glycerol Kinase and Glycerophosphate Oxidase (EC 3.1.1.3)
Enzymatic assay of lipase type XIII from Pseudomonas sp. using a coupled enzyme system of glycerol kinase and glycerophosphate oxidase (EC 3.1.1.3)
HPLC Analysis of Ascorbic Acid on Ascentis® RP-Amide
Separation of (L)-Dehydroascorbic acid; L-Ascorbic acid 20 μg/mL
USP HPLC Analysis of Ampicillin Sodium on Ascentis® Express C18
USP HPLC Analysis of Ampicillin Sodium on Ascentis® Express C18
HPLC Analysis of Aswagandha Extract on Discovery® C18
Separation of Withanoside IV; Withanoside V; Withaferin A; 12-Deoxywithastramonolide; Withanolide A; Withanolide B
Enzymatic Assay of β-Glucuronidase (EC 3.2.1.31) from E. coli
Objective: To standardize a procedure for the enzymatic assay of β-Glucuronidase.
Dexamethasone HPLC Assay and Impurity Profiling Methods Following United States Pharmacopeia Monograph Guidelines Using a Titan C18 Column and UV Detection
A simple, precise and sensitive Reverse-Phase High Pressure Liquid Chromatography gradient method was adapted for traceability, homogeneity and total chromatographic analysis of Dexamethasone. The given experimental conditions follow the USP43-NF38 monograph method for Dexamethasone Assay and Organic Impurity Profiling. Dexamethasone
Enzymatic Assay of Phytase (EC 3.1.3.8)
Derived from procedure SSPHYT02. Includes template updates to current SOP specifications and incorporation of notes into the procedure.
EPA Method 547: HPLC Analysis of Glyphosate and Glyphosate Degradation Product (Aminomethyl)phosphonic Acid (AMPA)
EPA Method 547 outlines the analysis of glyphosate in drinking water by direct aqueous injection HPLC, post column derivatization, and fluorescence detection
Aminoglycoside Analysis in Pork Muscle using Molecularly Imprinted Polymer Cleanup and LC/MS/MS Detection
Extraction and quantitative analysis of aminoglycosides in porcine tissue, using molecular imprinted polymer solid phase extraction followed by LC-MS/MS.
Buffer Reference Center
Choose and prepare a biological buffer based on your pH requirements as well as the pKa, a measure of acid strength that accounts for pH, concentration, and temperature.
Enzymatic Assay of D-Alanine Racemase (EC 5.1.1.1)
This procedure applies to all products that have a specification for the enzymatic assay of D Alanine Racemase.
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